CD34 is a cell-surface glycoprotein type 1 transmembrane protein that belongs to the sialomucin family. CD34 comprises of an intracellular cytoplasmic domain with consensus sites for serine, threonine, tyrosine and active protein kinase C (PKC). Because it is differentially glycosylated within different cell types, it has a range of apparent molecular weight sizes. It is a marker for pluripotent hematopoietic stem or progenitor cells and has been extensively used to isolate and characterize these progenitor cells. CD34 is also expressed on vascular endothelium, bone marrow stroma, embryonic fibroblasts, and neurons. As CD34-positive populations expand and differentiate into their destined lymphohematopoietic lineages, they lose their hallmark CD34 expression.
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Immunocytochemistry/Immunofluorescence: CD34 Antibody
A number of malignancies (certain sarcomas and fibromas, preB-ALL, peripheral nerve sheath tumors, and papillary thyroid carcinoma) appear to also exhibit CD34-positive expression. Endothelial cell (EC) isolation studies employed flow cytometry and a CD34 antibody from Dong’s lab demonstrate a rapid and reproducible strategy for isolating murine ECs that is free of contamination1. Zheng et al relied upon immunohistochemical with a CD34 antibody to validate their system of rapidly and reproducibly generating hair follicles from dissociated cells2. Their studies confirm that early developmental stages within an epithelial-mesenchymal system require an underlying epithelial structured platform. Sironi’s group at the Mario Negri Institute used the CD34 antibody, coupled with whole genome transcriptional profiling, to monitor their isolation and characterization of a promising endothelial cell line as a source of lymphatic endothelium3. Flow cytometry and immunohistochemistry with the CD34 antibody allowed Kalabis’ lab to identify a key stem cell population within the esophagus4. They characterized the self-renewing capacity of this particular cell cohort using a clonogenic assay and 3D organotypic culture model. Additionally, the CD34 antibody allowed Cambridge researchers to examine the role of endothelial hypoxia inducible transcription factors HIF1 and HIF2 in metastasis regulation and malignancy5.
Novus Biologicals offers CD34 reagents for your research needs including:
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