Home » Cell & Tissue Staining Kits

Cell & Tissue Staining Kits

IHC Tissue Microarrays

Cell & Tissue Staining Kits

Cell & Tissue Staining Kits provide all the supporting reagents required for immunohistochemistry staining of paraffin-embedded or cryostat tissue sections. Expression of target antigen in up to 50 samples can be visualized based on enzymatic conversion of chromogenic substrates 3,3’ Diaminobenzidine (DAB) or 3-amino-9-ethylcarbazole (AEC) into a colored precipitate. The use of high sensitivity horseradish peroxidase (HRP), in combination with signal amplification through the labeled streptavidin-biotin (LSAB) detection method, increase signal strength and reduce non-specific background. Briefly, the labeled streptavidin-biotin (LSAB) method uses a biotin conjugated secondary antibody to link the primary antibody to a streptavidin-peroxidase complex. The ability of each secondary antibody to form multiple complexes amplifies signal.

Key Benefits

  • Ready to use
  • High Sensitivity Streptavidin (HSS) increases detection sensitivity
  • Suitable for tissue sections & cytospin preparations
  • Pre-diluted reagents minimize hands-on time
  • Optimizes mouse, rabbit, rat, goat, or sheep IgG primary antibody staining

 

Kit Components
Blocking reagents To minimize non-specific Peroxidase, Avidin, and Biotin signals
Secondary antibodies Biotinylated and pre-diluted to minimize hands-on time
HSS-conjugated HRP Chemically optimized Streptavidin displays little or no non-specific binding to phospholipids, nucleic acids, and carbohydrate binding proteins
Chromogen Choose DAB (brown) or AEC (red)

View a complete list of available kits
 
in human lymph node

PD-1 in Human Lymph Node. PD-1 staining using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog# CTS008).

 Her2 in breast cancer tissue

Her2 in Breast Cancer Tissue. Optimized using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (Catalog # CTS008)