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Primary Conjugated Antibodies: Find Your Antibody and Conjugate

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Conjugated Primary Antibodies

A conjugated antibody (also known as a tagged or labeled antibody) is a monoclonal or polyclonal antibody that is coupled to a probe allowing for direct detection of a target antigen. Novus Biologicals offers primary antibodies for detection of over 7,400 targets directly conjugated to more than 25 fluorescent or enzymatic labels, including a wide spectrum of Alexa Fluor® dyes, PE and FITC conjugated antibodies. Novus conjugated primary antibodies will help you efficiently generate clean, reproducible results in a variety of applications such as Flow Cytometry, Immunocytochemistry (ICC), Immunohistochemistry (IHC), and ELISA.



Human tonsil tissue was stained with Janelia Fluor® 549 conjugated Anti-Alpha Smooth Muscle Actin (SMA) (red) and counterstained with DAPI (blue).

Immunohistochemistry-Frozen. Human tonsil tissue was stained with Janelia Fluor® 549 conjugated Anti-Alpha Smooth Muscle Actin (SMA) (Catalog # NBP2-34522JF549) (red) and counterstained with DAPI (blue). Image from verified customer review.


Pseudo color flow cytometry plot showing mouse splenocytes stained with lymphocyte markers B220/CD45R and CD3.

Flow Cytometry-Mouse. Pseudo color flow cytometry plot showing mouse splenocytes stained with lymphocyte markers B220/CD45R and CD3. Surface stain was performed with an antibody cocktail of Allophycocyanin (APC)-conjugated Anti-Mouse CD3 (Catalog # FAB4841A) and either (A) Alexa Fluor® 405 conjugated Anti-Mouse B220/CD45R (Catalog # FAB1217V) or (B) Alexa Fluor® 405-conjugated Rat IgG2a Isotype Control (Catalog # IC006V).



Popular Conjugates for Primary Antibodies

Excitation Laser (nm) Conjugate Excitation Max (nm) Emission Max (nm) Emission Color
    Biotin    
    HRP    
UV (350)         Alexa Fluor® 350 346 442     
UV (350)         DyLight 350 353 432     
Violet (405)      DyLight 405 400 420     
Violet (405)         Alexa Fluor® 405 401 421     
Violet (405)      mFluorTM Violet 450 406 445 New!
Violet (405)      mFluorTM Violet 500 410 501 New!
Violet (405)      mFluorTM Violet 610 421 613 New!
Blue (488)         Alexa Fluor® 488 490 525     
Blue (488)         DyLight 488 493 518     
Blue (488)         FITC 498 519     
Blue (488)    Y-G (561)    PE 498, 565 578     
Blue (488)    Y-G (561)    PE/Atto594 498,566 632     
Blue (488)         PerCP 482 678     
Blue (488)    Y-G (561)    PE/Cy5.5 498, 566 700     
Blue (488)    Y-G (561)    PE/Cy7 489, 566 782     
Y-G (561)         Alexa Fluor® 532 532 554     
Y-G (561)         Janelia Fluor® 549 549 571     
Y-G (561)         DyLight 550 562 576     
Y-G (561)         Alexa Fluor® 594 590 617     
Y-G (561)         DyLight 594 593 618     
Red (633,640)         APC 650 660     
Red (633,640)         Janelia Fluor® 646 646 664     
Red (633,640)         DyLight 650 652 672     
Red (633,640)         Alexa Fluor® 647 653 669     
Red (633,640)         DyLight 680 692 712     
Red (633,640)         Alexa Fluor® 700 696 720     
Red (633,640)         Alexa Fluor® 750 749 775     
Red (633,640)         APC/Cy7 652 790     

Key: 'Y-G'= Yellow-Greeen

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Conjugated Antibody Support

Novus Spectra Viewer
Visualize the spectral profile of the fluorescent probes, either individually or in combination to identify optimal fluorophore conjugates for a multicolor imaging or flow cytometry experiment.

Novus Panel Builder
Streamline the process of building a multiparametric flow cytometry panel. Select a cytometer configuration from the list of available institutions or customize the configuration from default cytometer settings to choose compatible fluorochromes.

Flow Cytometry Handbook
Our step-by-step guide provides information on the basics of flow cytometry and troubleshooting tips.

Custom Antibody Conjugation Services
Don’t see a conjugated option to a target of interest? Novus provides custom conjugation services for many of our available primary antibodies to meet individual researcher’s needs.

Conjugated Antibodies for Research Areas

 

T98G glioblastoma cells stained with Alexa Fluor® 647-conjugated Anti-LAMP-2/CD107b (red), Cathepsin D (green), and counterstained with DAPI (blue)

Immunocytochemistry/Immunofluorescence. T98G glioblastoma cells were stained with Alexa Fluor® 647-conjugated Anti-LAMP-2/CD107b (Catalog # NBP2-22217AF647) (red), Cathepsin D (green), and counterstained with DAPI (blue). Image from a verified customer review.

Pseudo color flow cytometry plot showing peripheral blood monocytes co-stained with CD14 and CD163

Flow Cytometry-Human. Pseudo color flow cytometry plot showing peripheral blood monocytes co-stained with CD14 and CD163. Surface stain was performed on human PBMCs with Alexa Fluor® 488 conjugated Anti-CD14 (Catalog # NB100-77758AF488) and either (left) Allophycocyanin (APC) conjugated Anti-CD163 (Catalog # NB110-40686APC) or (right) Allophycocyanin (APC) conjugated Human IgG1 isotype control.


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