OLLAS Epitope Tag System

The OLLAS (E.coli OmpF Linker and mouse Langerin fusion Sequence) tag is a new, highly sensitive epitope tag. OLLAS tags are detectable using the OLLAS monoclonal antibody at approximately 100-fold lower levels than similar commercially available tags, including Sigma's FLAG™ epitope tag system. The OLLAS tag has also been demonstrated to provide superior functionality whether used as an N-terminal, C-terminal, or internal tag on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.  The OLLAS antibody has recently been used in 2 publications:

1. Beckett K, Monier S, Palmer L et al Drosophila S2 Cells Secrete Wingless on Exosome-Like Vesicles but the Wingless Gradient Forms Independently of Exosomes Traffic 2012 Oct 5 [PMID: 23035643] (Drosophila)
2. Jerlstrom-Hultqvist J, Einarsson E, Svärd SG Stable transfection of the diplomonad parasite Spironucleus salmonicida Eukaryot Cell 2012 Sep 14 [PMID: 22983987] (ICC/IF)

Western blot: pCMV-SD OLLAS [NBP1-06713] - comparison of binding sensitivity of Novus Biologicals' monoclonal
antibodies to OLLAS (NBP1-06713), DYKDDDDK (NBP1-06712) and the FLAG-M2 monoclonal antibody from Sigma-Aldrich.
FLAG™ and ANTI-FLAG™ are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Antibodies

• OLLAS Epitope Tag Antibody (NBP1-06713)
• OLLAS Epitope Tag Antibody [Agarose Immobilized] (NBP1-30083)

Vectors

• N OLLAS Vector (NBA1-07083) - N-OLLAS vector is for the intracellular expression of N-terminally OLLAS-tagged protein.
  
• ND OLLAS/DYKDDDDK Vector (NBA1-07084) - ND-OLLAS vector for the intracellular expression of N-terminally FLAG(DYKDDDDK) and OLLAS dual tandem tagged protein.
  
• SD OLLAS/DYKDDDDK Vector (NBA1-07085) - SD-OLLAS vector for the expression of secretory protein with N-terminally FLAG(DYKDDDDK) and OLLAS dual tandem tagged.

Kits

• N OLLAS Epitope Tag Kit (NBK1-18296) (contains OLLAS primary antibody and N OLLAS Vector)
  
• ND OLLAS/DYKDDDDK Epitope Tag Kit (NBK1-19033) (contains OLLAS primary antibody and ND OLLAS/DYKDDDDK vector)
  
• SD OLLAS/DYKDDDDK Epitope Tag Kit (NBK1-19035) (contains OLLAS primary antibody and SD OLLAS/DYKDDDDK vector)

Original References

1. Park SH, Cheong C, Idoyaga J, et al. Generation and application of new rat monoclonal antibodies against synthetic FLAG and OLLAS tags for improved immunodetection. J Immunol Methods. 2008 Feb 29;331(1-2):27-38. [PMID: 18054954]
2. Idoyaga J, Cheong C, Suda K,  et al. Cutting edge: langerin/CD207 receptor on dendritic cells mediates efficient antigen presentation on MHC I and II products in vivo. J Immunol. 2008 Mar 15;180(6):3647-50. [PMID: 18322168]