12-Lipoxygenase Antibody (2D10) - Azide and BSA Free

ELISA: 12-Lipoxygenase Antibody (2D10) [H00000239-M01] - Detection limit for recombinant GST tagged ALOX12 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA
• Clone: 2D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

12-Lipoxygenase Antibody (2D10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ALOX12 (NP_000688, 564 a.a. ~ 663 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPPTTKEDVTMATVMGSLPDVRQACLQMAISWHLSRRQPDMVPLGHHKEKYFSGPKPKAVLNQFRTDLEKLEKEITARNEQLDWPYEYLKPSCIENSVTI
• Specificity: ALOX12 - arachidonate 12-lipoxygenase (2D10)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALOX12
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for 12-Lipoxygenase Antibody (2D10) - Azide and BSA Free

• 12(S)-lipoxygenase
• 12-Lipoxygenase
• 12LO
• 12-LOX
• 12S-lipoxygenase
• 12S-LOX
• 12S-LOXarachidonate 12-lipoxygenase, 12S-type
• ALOX12
• arachidonate 12-lipoxygenase
• EC 1.13.11
• EC 1.13.11.-
• EC 1.13.11.31
• EC 1.13.11.33
• EC 3.3.2.-
• LOG12
• platelet 12-LOX
• platelet-type 12-lipoxygenase
• Platelet-type lipoxygenase 12

Background

Oxygenase and 14,15-leukotriene A4 synthase activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 12-Lipoxygenase Antibody (H00000239-M01)(2)

Publications using H00000239-M01

• Gregus AM, Dumlao DS, Wei SC et al. Systematic analysis of rat 12/15-lipoxygenase enzymes reveals critical role for spinal eLOX3 hepoxilin synthase activity in inflammatory hyperalgesia. FASEB J 2013-02-04 [PMID: 23382512]
• Gabrielle Fredman, Yongsheng Li, Jesmond Dalli, Nan Chiang, Charles N. Serhan Self-Limited versus Delayed Resolution of Acute Inflammation: Temporal Regulation of Pro-Resolving Mediators and MicroRNA Scientific Reports 2012-09-06 [PMID: 22957142]

Bioinformatics

• Gene Symbol: ALOX12
• Entrez:
  • 239(Human)
• Uniprot:
  • NP_000688(Human)