14-3-3 sigma/Stratifin Antibody (SD2070)

Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody (SD2070) [NBP2-67771] - Analysis of paraffin-embedded human tonsil tissue using anti-14-3-3 sigma antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody (SD2070) [NBP2-67771] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-14-3-3 sigma antibody. Counter stained with hematoxylin.

Product Discontinued

14-3-3 sigma/Stratifin Antibody (SD2070) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human 14-3-3 sigma/Stratifin 50-99 / 248. (SwissProt: P31947 Human; Entrez Gene: 313017 Rat)
• Localization: Cytoplasm, Nucleus, Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SFN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for 14-3-3 sigma/Stratifin Antibody (SD2070)

• 14-3-3 protein sigma
• 1433 sigma
• 14-3-3 sigma
• Epithelial cell marker protein 1
• HME1
• SFN
• stratifin
• YWHAS

Background

14-3-3 sigma was identified as an epithelial cell marker. It is part of the 14-3-3 family of proteins in which seven isoforms have been identified: beta, zeta, gamma, eta, epsilon, tau, and sigma. 14-3-3 proteins function as adaptors that bind with a number of partners to mediate various signaling pathways. 14-3-3 sigma is also called stratifin or HME1 and appears to function as a tumor suppressor whose expression can be downregulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis. Alternate names for 14-3-3 sigma include epithelial marker protein 1, SFN and YWHAS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SFN