4-Hydroxynonenal Antibody (198960) [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE). PVDF membrane was probed with 1 µg/mL of Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for 4-Hydroxynonenal at adducts of histidine residues (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE), loaded at 0.2 mg/mL. Specific bands were detected for 4‑Hydroxynonenal at adducts of histidine residues kDa (as indicated) using 5 µg/mL of Mouse Anti-4‑Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

4‑Hydroxynonenal was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-4‑Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

4‑Hydroxynonenal was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-4‑Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Diapocynin inhibits the formation of 3-nitrotyrosine (3-NT) and 4-hydroxynonenal (4-HNE) in the substantia nigra (SN) of MPTP-treated mice. (A) Representative Western blots illustrating the expression of 3-NT in SN. (B) Bar graph showing mean Western blot 3-NT/ beta -actin ratios ± SEM in SN of 6 mice per group. (C) Double labeling of tyrosine hydroxylase (TH) and 3-NT in SN region of ventral midbrain. (D) Representative Western blots illustrating the expression of 4-HNE in SN. (E) Bar graph showing mean Western blot 4-HNE/ beta -actin ratios ± SEM in SN of 6 mice per group. (F) Double labeling of TH and 4-HNE in SN region of ventral midbrain. Images were captured at 60× magnification. The SN zone is outlined in white dots. Inset pictures demonstrated colocalization of TH and 3-NT/4-HNE. ***P <0.001 vs the control group; **P <0.01 vs the control group; *P <0.05 vs the MPTP group; #P <0.001 vs the MPTP group. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23092448), licensed under a CC-BY license. Not internally tested by R&D Systems.

Diapocynin inhibits the formation of 3-nitrotyrosine (3-NT) and 4-hydroxynonenal (4-HNE) in the substantia nigra (SN) of MPTP-treated mice. (A) Representative Western blots illustrating the expression of 3-NT in SN. (B) Bar graph showing mean Western blot 3-NT/ beta -actin ratios ± SEM in SN of 6 mice per group. (C) Double labeling of tyrosine hydroxylase (TH) and 3-NT in SN region of ventral midbrain. (D) Representative Western blots illustrating the expression of 4-HNE in SN. (E) Bar graph showing mean Western blot 4-HNE/ beta -actin ratios ± SEM in SN of 6 mice per group. (F) Double labeling of TH and 4-HNE in SN region of ventral midbrain. Images were captured at 60× magnification. The SN zone is outlined in white dots. Inset pictures demonstrated colocalization of TH and 3-NT/4-HNE. ***P <0.001 vs the control group; **P <0.01 vs the control group; *P <0.05 vs the MPTP group; #P <0.001 vs the MPTP group. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23092448), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: All-Multi
• Applications: WB, Simple Western, IHC
• Clone: 198960
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

4-Hydroxynonenal Antibody (198960) [Unconjugated] Summary

• Immunogen: KLH-coupled 4-Hydroxynonenal
• Specificity: Detects 4-Hydroxynonenal adducts of histidine residues. No cross-reactivity with nitrotyrosine, formaldehyde, glutaraldehyde, or 4‑hydroxy hexenaldehyde adducts is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.1-25 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 4-Hydroxynonenal Antibody (198960) [Unconjugated]

• (2E)-4-Hydroxy-2-nonenal
• 2-Nonenal, 4-hydroxy-, (2E,4R)-
• 4 HNE
• 4Hydroxynonenal
• 4-Hydroxynonenal
• 4-hydroxy-trans-2-nonenal
• HNE

Background

4-hydroxy-2-nonenal (4-hydroxynonenal, 4-HNE) is a highly reactive aldehyde generated by the exposure of polyunsaturated fatty acids to peroxides and reactive oxygen species (ROS). It non-enzymatically forms stable protein adducts with histidine, lysine, and cysteine side chains that have been used as biomarkers for oxidative damage in cells. Conditions where 4-HNE immunoreactivity has been observed include include inflammation, neurodegenerative diseases, and ischemic damage to the heart and brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 4-Hydroxynonenal Antibody (MAB3249)(111)

Publications using MAB3249

• Harrer, A;Ghatpande, N;Grimaldini, T;Fietz, D;Kumar, V;Pleuger, C;Fijak, M;Föppl, DT;Rynio, LP;Schuppe, HC;Pilatz, A;Bartkuhn, M;Procida-Kowalski, T;Guttmann-Raviv, N;Bhushan, S;Meyron-Holtz, EG;Meinhardt, A; Iron regulatory protein 1 deficient mice exhibit hypospermatogenesis The Journal of biological chemistry 2024-12-10 [PMID: 39667502] (Western Blot, Mouse)
• Choudhury, TZ;Greskovich, SC;Girard, HB;Rao, AS;Budhathoki, Y;Cameron, EM;Conroy, S;Li, D;Zhao, MT;Garg, V; Impact of genetic factors on antioxidant rescue of maternal diabetes-associated congenital heart disease JCI insight 2024-10-22 [PMID: 39437002] (Immunohistochemistry, Mouse)
• Zhao, Z;Chen, M;Sun, K;Gu, X; CD8+ T cell associated scoring model helps prognostic diagnosis and immunotherapy selection in patients with colon adenocarcinoma Heliyon 2024-10-15 [PMID: 39386801] (Immunohistochemistry, Human, Mouse)
• Joubert, MBV;Ingaramo, PI;Collins, P;D'Alessandro, ME; Astaxanthin improves lipotoxicity, lipid peroxidation and oxidative stress in kidney of sucrose-rich diet-fed rats The Journal of nutritional biochemistry 2024-10-05 [PMID: 39374743] (Immunohistochemistry, Rat)
• Yang, X;Bhowmick, K;Rao, S;Xiang, X;Ohshiro, K;Amdur, RL;Hassan, MI;Mohammad, T;Crandall, K;Cifani, P;Shetty, K;Lyons, SK;Merrill, JR;Vegesna, AK;John, S;Latham, PS;Crawford, JM;Mishra, B;Dasarathy, S;Wang, XW;Yu, H;Wang, Z;Huang, H;Krainer, AR;Mishra, L; Aldehydes alter TGF-? signaling and induce obesity and cancer Cell reports 2024-08-31 [PMID: 39217614] (Immunohistochemistry, Western Blot, Human)
• Kargi-Gemici, E;?engelen, A;Aksüt, Y;Akyol, O;?engiz-Erhan, S;Bay, M;Önay-Uçar, E;Selcan, A;Demirgan, S; Cerium oxide nanoparticles (nanoceria) pretreatment attenuates cell death in the hippocampus and cognitive dysfunction due to repeated isoflurane anesthesia in newborn rats Neurotoxicology 2024-08-29 [PMID: 39216603] (Western Blot, Rat)
• Cui, Y;Li, Y;Xu, Y;Liu, X;Kang, X;Zhu, J;Long, S;Han, Y;Xue, C;Sun, Z;Du, Y;Hu, J;Pan, L;Zhou, F;Xu, X;Li, X; SLC7A11 protects luminal A breast cancer cells against ferroptosis induced by CDK4/6 inhibitors Redox biology 2024-08-10 [PMID: 39153252] (Immunohistochemistry, Xenograft)
• Wu, L;Lin, H;Li, S;Huang, Y;Sun, Y;Shu, S;Luo, T;Liang, T;Lai, W;Rao, J;Hu, Z;Peng, H; Macrophage iron dyshomeostasis promotes aging-related renal fibrosis Aging cell 2024-07-17 [PMID: 39016438] (Immunohistochemistry, Mouse)
• Wang, P;Ouyang, J;Zhou, K;Hu, D;Zhang, S;Zhang, A;Yang, Y; Olesoxime protects against cisplatin-induced acute kidney injury by attenuating mitochondrial dysfunction Biomedical journal 2024-04-19 [PMID: 38643825] (Immunohistochemistry, Mouse)
• Agius, T;Emsley, R;Lyon, A;MacArthur, MR;Kiesworo, K;Faivre, A;Stavart, L;Lambelet, M;Legouis, D;de Seigneux, S;Golshayan, D;Lazeyras, F;Yeh, H;Markmann, JF;Uygun, K;Ocampo, A;Mitchell, SJ;Allagnat, F;Déglise, S;Longchamp, A; Short-term hypercaloric carbohydrate loading increases surgical stress resilience by inducing FGF21 Nature communications 2024-02-05 [PMID: 38316771] (Immunohistochemistry, Mouse)
• Bae, CR;Kim, Y;Kwon, YG; CU06-1004 alleviates oxidative stress and inflammation on folic acid-induced acute kidney injury in mice Journal of pharmacological sciences 2024-02-01 [PMID: 38246731] (Immunohistochemistry, Mouse)
• Pan, X;Huang, P;Ali, SS;Renslo, B;Hutchinson, TE;Erwin, N;Greenberg, Z;Ding, Z;Li, Y;Warnecke, A;Fernandez, NE;Staecker, H;He, M; CRISPR-Cas9 Engineered Extracellular Vesicles for the Treatment of Dominant Progressive Hearing Loss bioRxiv : the preprint server for biology 2023-12-15 [PMID: 38168224] (Immunohistochemistry, Mouse)
• Lee, KY;Yang, CC;Shueng, PW;Wu, SM;Chen, CH;Chao, YC;Chang, YC;Han, CL;Chuang, HC;Lee, CC;Lin, CW; Downregulation of TAZ elicits a mitochondrial redox imbalance and ferroptosis in lung epithelial cells exposed to diesel exhaust particles Ecotoxicology and environmental safety 2023-10-11 [PMID: 37832483] (IHC, Rats, Human)
• Fanfeng Chen, Jiayu Zhan, Mi Liu, Abdullah Al Mamun, Shanshan Huang, Yibing Tao et al. FGF2 Alleviates Microvascular Ischemia-Reperfusion Injury by KLF2-mediated Ferroptosis Inhibition and Antioxidant Responses International Journal of Biological Sciences 8/21/2023 [PMID: 37705747]
• Tognoloni, A;Bartolini, D;Pepe, M;Di Meo, A;Porcellato, I;Guidoni, K;Galli, F;Chiaradia, E; Platelets Rich Plasma Increases Antioxidant Defenses of Tenocytes via Nrf2 Signal Pathway International journal of molecular sciences 2023-08-27 [PMID: 37686103] (Western Blot, Equine)
• Jean Wilson, E;Sirpu Natesh, N;Ghadermazi, P;Pothuraju, R;Shanmugam, M;Prajapati, DR;Pandey, S;Kaifi, JT;Dodam, JR;Bryan, J;Lorson, CL;Watrelot, AA;Foster, JM;Mansel, TJ;Joshua Chan, SH;Batra, SK;Subbiah, J;Rachagani, S; Red cabbage juice-mediated gut microbiota modulation improves intestinal epithelial homeostasis and ameliorates colitis bioRxiv : the preprint server for biology 2023-08-24 [PMID: 37662255] (IHC, Mouse)
• Wang, C;Xie, L;Xing, Y;Liu, M;Yang, J;Gao, N;Cai, Y; Iron-overload-induced ferroptosis in mouse cerebral toxoplasmosis promotes brain injury and could be inhibited by Deferiprone PLoS neglected tropical diseases 2023-08-31 [PMID: 37651502] (Western Blot, Mouse)
• Choi, J;Choi, H;Chung, J; Icariin Supplementation Suppresses the Markers of Ferroptosis and Attenuates the Progression of Nonalcoholic Steatohepatitis in Mice Fed a Methionine Choline-Deficient Diet International journal of molecular sciences 2023-08-07 [PMID: 37569885] (Western Blot, Mouse)
• Deng, Z;Wang, Y;Liu, J;Zhang, H;Zhou, L;Zhao, H;Han, Y;Yan, S;Dong, Z;Wang, Y;Dai, Y;Deng, F; WBP2 restrains the lysosomal degradation of GPX4 to inhibit ferroptosis in cisplatin-induced acute kidney injury Redox biology 2023-07-26 [PMID: 37516014] (ICC, Mouse)
• Enrico Radaelli, Charles-Antoine Assenmacher, Jillian Verrelle, Esha Banerjee, Florence Manero, Salim Khiati et al. Mitochondrial defects leading to arrested spermatogenesis and ferroptosis in the PARL deficient mouse model of Leigh Syndrome eLife 2023-07-28 [PMID: 37505079] (Western Blot, Mouse, Transgenic Mouse)
• Wen H, Chen J, Zhao B et al. Repression of ferroptotic cell death by mitochondrial calcium signaling Research square 2023-07-10 [PMID: 37502961]
• Shi J, Wang QH, Wei X et al. Histone acetyltransferase P300 deficiency promotes ferroptosis of vascular smooth muscle cells by activating the HIF-1?/HMOX1 axis Molecular medicine (Cambridge, Mass.) 2023-07-06 [PMID: 37415103]
• Mankowski R, Wohlgemuth S, Bresciani G et al. Intraoperative Hemi-Diaphragm Electrical Stimulation Demonstrates Attenuated Mitochondrial Function without Change in Oxidative Stress in Cardiothoracic Surgery Patients Antioxidants 2023-04-27 [PMID: 37237876] (Simple Western)
• Jianglin Zheng, Qing Zhang, Zhen Zhao, Yue Qiu, Yujie Zhou, Zhipeng Wu et al. Epigenetically silenced lncRNA SNAI3-AS1 promotes ferroptosis in glioma via perturbing the m6A-dependent recognition of Nrf2 mRNA mediated by SND1 Journal of Experimental & Clinical Cancer Research 2023-05-19 [PMID: 37202791]
• Yang T, Li Q, Fadoul G et al. Aldo-Keto Reductase 1C15 Characterization and Protection in Ischemic Brain Injury Antioxidants (Basel, Switzerland) 2023-04-11 [PMID: 37107284]
• C Chen, Y Yang, Y Guo, J He, Z Chen, S Qiu, Y Zhang, H Ding, J Pan, Y Pan CYP1B1 inhibits ferroptosis and induces anti-PD-1 resistance by degrading ACSL4 in colorectal cancer Cell Death & Disease, 2023-04-14;14(4):271. 2023-04-14 [PMID: 37059712] (IHC, Mouse)
• YJ Kahm, IG Kim, RK Kim RanBP1: A Potential Therapeutic Target for Cancer Stem Cells in Lung Cancer and Glioma International Journal of Molecular Sciences, 2023-04-06;24(7):. 2023-04-06 [PMID: 37047826] (IHC, Western Blot, Mouse)
• Y Zha, Y Yang, Y Zhou, B Ye, H Li, J Liang Dietary Evodiamine Inhibits Atherosclerosis-Associated Changes in Vascular Smooth Muscle Cells International Journal of Molecular Sciences, 2023-04-03;24(7):. 4/3/2023 [PMID: 37047626]
• Yi-Wei Du, Xiao-Kang Li, Ting-Ting Wang, Lu Zhou, Hui-Rong Li, Lan Feng et al. Cyanidin-3-glucoside inhibits ferroptosis in renal tubular cells after ischemia/reperfusion injury via the AMPK pathway Molecular Medicine 2023-04-03 [PMID: 37013504]
• Xing Du, Xiaolong Ma, Ying Tan, Fangyu Shao, Chun Li, Yang Zhao et al. B cell-derived anti-beta 2 glycoprotein I antibody mediates hyperhomocysteinemia-aggravated hypertensive glomerular lesions by triggering ferroptosis Signal Transduction and Targeted Therapy 2023-03-13 [PMID: 36907919]
• Qingyu Zhang, Ning Li, Limei Deng, Xingmei Jiang, Yuming Zhang, Leo Tsz On Lee et al. ACSL1-induced ferroptosis and platinum resistance in ovarian cancer by increasing FSP1 N-myristylation and stability Cell Death Discovery 2023-03-08 [PMID: 36882396]
• YJ Chiu, CH Lin, CY Lin, PN Yang, YS Lo, YC Chen, CM Chen, YR Wu, CF Yao, KH Chang, GJ Lee-Chen Investigating Therapeutic Effects of Indole Derivatives Targeting Inflammation and Oxidative Stress in Neurotoxin-Induced Cell and Mouse Models of Parkinson&#039;s Disease International Journal of Molecular Sciences, 2023-01-30;24(3):. 2023-01-30 [PMID: 36768965] (IHC, Mouse)
• YJ Chiu, CH Lin, CY Lin, PN Yang, YS Lo, YC Chen, CM Chen, YR Wu, CF Yao, KH Chang, GJ Lee-Chen Investigating Therapeutic Effects of Indole Derivatives Targeting Inflammation and Oxidative Stress in Neurotoxin-Induced Cell and Mouse Models of Parkinson&#039;s Disease International Journal of Molecular Sciences, 2023;24(3):. 2023 [PMID: 36768965] (IHC, Mouse)
• Du C, Xu H, Cao C et al. Neutral amino acid transporter SLC38A2 protects renal medulla from hyperosmolarity-induced ferroptosis eLife 2023-02-01 [PMID: 36722887]
• Yu Sun, Hadrien Demagny, Adrien Faure, Francesca Pontanari, Antoine Jalil, Nadia Bresciani et al. Asparagine protects pericentral hepatocytes during acute liver injury Journal of Clinical Investigation 2023-04-03 [PMID: 36719750]
• Yu-Hsuan Chen, Jinn-Yuan Hsu, Ching-Tung Chu, Yao-Wen Chang, Jia-Rong Fan, Muh-Hwa Yang et al. Loss of cell–cell adhesion triggers cell migration through Rac1-dependent ROS generation Life Science Alliance 2/1/2023 [PMID: 36446524]
• Yu-Hsuan Chen, Jinn-Yuan Hsu, Ching-Tung Chu, Yao-Wen Chang, Jia-Rong Fan, Muh-Hwa Yang et al. Loss of cell–cell adhesion triggers cell migration through Rac1-dependent ROS generation Life Science Alliance 2023-02-01 [PMID: 36446524] (Immunohistochemistry, Human)
• Lars Saemann, Fabio Hoorn, Adrian-Iustin Georgevici, Sabine Pohl, Sevil Korkmaz-Icöz, Gábor Veres et al. Cytokine Adsorber Use during DCD Heart Perfusion Counteracts Coronary Microvascular Dysfunction Antioxidants (Basel) 2022-11-17 [PMID: 36421466]
• Ban K, Nam G, Kim D et al. Prevention of LPS-Induced Acute Kidney Injury in Mice by Bavachin and Its Potential Mechanisms Antioxidants 10/24/2022 [PMID: 36358467]
• Ali Mahdi, Tigist Wodaje, Oskar Kövamees, John Tengbom, Allan Zhao, Tong Jiao et al. The red blood cell as a mediator of endothelial dysfunction in patients with familial hypercholesterolemia and dyslipidemia Journal of Internal Medicine 2023-02-01 [PMID: 36324273]
• SCW Sze, L Zhang, S Zhang, K Lin, TB Ng, ML Ng, KF Lee, JKW Lam, Z Zhang, KKL Yung Aberrant Transferrin and Ferritin Upregulation Elicits Iron Accumulation and Oxidative Inflammaging Causing Ferroptosis and Undermines Estradiol Biosynthesis in Aging Rat Ovaries by Upregulating NF-kappab-Activated Inducible Nitric Oxide Synthase: First Demonstration of an Intricate Mechanism International Journal of Molecular Sciences, 2022-10-21;23(20):. 2022-10-21 [PMID: 36293552] (Western Blot, Rat)
• Ling-Yu Yang, Yong-Ren Chen, Jing-Er Lee, Kuo-Wei Chen, Hui-Tzung Luh, Yi-Tzu Chen et al. Dental Pulp Stem Cell-Derived Conditioned Medium Alleviates Subarachnoid Hemorrhage-Induced Microcirculation Impairment by Promoting M2 Microglia Polarization and Reducing Astrocyte Swelling Translational Stroke Research 2023-10-01 [PMID: 36181630] (Immunohistochemistry, Western Blot, Immunocytochemistry, Rat)
• Nicole P. Kasica, Xueyan Zhou, Hannah M. Jester, Caroline E. Holland, Alexey G. Ryazanov, Tom E. Forshaw et al. Homozygous knockout of eEF2K alleviates cognitive deficits in APP/PS1 Alzheimer’s disease model mice independent of brain amyloid beta pathology Frontiers in Aging Neuroscience 2022-09-09 [PMID: 36158543]
• P Siriviriya, J Sriko, K Somanawat, M Chayanupat, N Klaikeaw, D Werawatgan Genistein attenuated oxidative stress, inflammation, and apoptosis in L-arginine induced acute pancreatitis in mice BMC complementary medicine and therapies, 2022-08-04;22(1):208. 2022-08-04 [PMID: 35927726] (IHC, Mouse)
• N Li, X Yi, Y He, B Huo, Y Chen, Z Zhang, Q Wang, Y Li, X Zhong, R Li, XH Zhu, Z Fang, X Wei, DS Jiang Targeting Ferroptosis as a Novel Approach to Alleviate Aortic Dissection Oncogene, 2022-06-21;18(10):4118-4134. 2022-06-21 [PMID: 35844806] (IHC, Human)
• Griffin J, Krolikowski J, Kounga K et al. Red Light Mitigates the Deteriorating Placental Extracellular Matrix in Late Onset of Preeclampsia and Improves the Trophoblast Behavior Journal of Pregnancy 4/20/2022 [PMID: 35494491]
• C Liu, W Sun, T Zhu, S Shi, J Zhang, J Wang, F Gao, Q Ou, C Jin, J Li, JY Xu, J Zhang, H Tian, GT Xu, L Lu Glia maturation factor-beta induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy Redox Biology, 2022-03-18;52(0):102292. 2022-03-18 [PMID: 35325805] (Immunohistochemistry, Rat)
• Yu‐Fu Wu, Eduardo A. De La Toba, Svyatoslav Dvoretskiy, Rebecca Jung, Noah Kim, Laureen Daniels et al. Development of a cell‐free strategy to recover aged skeletal muscle after disuse The Journal of Physiology 2022-04-15 [PMID: 35318675] (Western Blot, Mouse)
• Yu‐Fu Wu, Eduardo A. De La Toba, Svyatoslav Dvoretskiy, Rebecca Jung, Noah Kim, Laureen Daniels et al. Development of a cell‐free strategy to recover aged skeletal muscle after disuse The Journal of Physiology 4/15/2022 [PMID: 35318675]
• Wang J, Wang Y, Liu Y et al. Ferroptosis, a new target for treatment of renal injury and fibrosis in a 5/6 nephrectomy-induced CKD rat model Cell death discovery 3/22/2022 [PMID: 35318301]
• Qinfeng Hu, Hang Chen, Cheng Shen, Beijian Zhang, Xinyu Weng, Xiaolei Sun et al. Impact and potential mechanism of effects of chronic moderate alcohol consumption on cardiac function in aldehyde dehydrogenase 2 gene heterozygous mice Alcoholism: Clinical and Experimental Research 2022-05-01 [PMID: 35315077]
• M Baškovi?, D Krsnik, M Himelreich, A Katuši? Bo, N Sin?i?, Z Sonicki, D Ježek Astaxanthin Relieves Testicular Ischemia-Reperfusion Injury-Immunohistochemical and Biochemical Analyses Journal of Clinical Medicine, 2022-02-26;11(5):. 2022-02-26 [PMID: 35268375] (IHC, Rat)
• Michael MacLean, Raquel López-Díez, Carolina Vasquez, Paul F. Gugger, Ann Marie Schmidt Neuronal–glial communication perturbations in murine SOD1G93A spinal cord Communications Biology 2022-02-28 [PMID: 35228715]
• Marta Moya, Leticia López-Valencia, Borja García-Bueno, Laura Orio Disinhibition-Like Behavior Correlates with Frontal Cortex Damage in an Animal Model of Chronic Alcohol Consumption and Thiamine Deficiency Biomedicines 2022-01-25 [PMID: 35203470]
• G Dang, T Li, D Yang, G Yang, X Du, J Yang, Y Miao, L Han, X Ma, Y Song, B Liu, X Li, X Wang, J Feng T lymphocyte-derived extracellular vesicles aggravate abdominal aortic aneurysm by promoting macrophage lipid peroxidation and migration via pyruvate kinase muscle isozyme 2 Redox Biology, 2022-02-04;50(0):102257. 2022-02-04 [PMID: 35149342] (IHC, Mouse)
• HH Dieguez, JS Calanni, HE Romeo, A Alaimo, MF González F, A Iaquinandi, MS Chianelli, MI Keller Sar, PH Sande, RE Rosenstein, D Dorfman Enriched environment and visual stimuli protect the retinal pigment epithelium and photoreceptors in a mouse model of non-exudative age-related macular degeneration Cell Death & Disease, 2021-12-04;12(12):1128. 2021-12-04 [PMID: 34864827] (IHC, Mouse)
• Feng Yang, Anpei Hu, Yanhua Guo, Jianqun Wang, Dan Li, Xiaojing Wang et al. p113 isoform encoded by CUX1 circular RNA drives tumor progression via facilitating ZRF1/BRD4 transactivation Molecular Cancer 2021-12-01 [PMID: 34579723]
• Xueying Ren, Yanchun Li, Yi Zhou, Wanye Hu, Chen Yang, Qiangan Jing et al. Overcoming the compensatory elevation of NRF2 renders hepatocellular carcinoma cells more vulnerable to disulfiram/copper-induced ferroptosis Redox Biology 2021-10-01 [PMID: 34482117]
• Lin Wang, Wenzhu Zhang, Ning Zou, Lijuan Zhang Trophoblasts Modulate the Ca2+ Oscillation and Contraction of Myometrial Smooth Muscle Cells by Small Extracellular Vesicle- (sEV-) Mediated Exporting of miR-25-3p during Premature Labor Oxidative Medicine and Cellular Longevity 2021-08-07 [PMID: 34413928]
• Oke S, Lee K, Papp R Et Al. In Utero Exposure to 9-Tetrahydrocannabinol Leads to Postnatal Catch-up Growth and Dysmetabolism in the Adult Rat Liver preprints 6/30/2021 [PMID: 34299119]
• Jing Du, Xu Wang, Yanchun Li, Xueying Ren, Yi Zhou, Wanye Hu et al. DHA exhibits synergistic therapeutic efficacy with cisplatin to induce ferroptosis in pancreatic ductal adenocarcinoma via modulation of iron metabolism Cell Death & Disease 2021-07-15 [PMID: 34262021] (Immunocytochemistry, Human)
• Jung-Hyo Cho, Jong-Suk Lee, Hyeong-Geug Kim, Hye Won Lee, Zhigang Fang, Hyeok-Hee Kwon et al. Ethyl Acetate Fraction of Amomum villosum var. xanthioides Attenuates Hepatic Endoplasmic Reticulum Stress-Induced Non-Alcoholic Steatohepatitis via Improvement of Antioxidant Capacities Antioxidants (Basel) 2021-06-23 [PMID: 34201527]
• D Das, A Paul, A Lahiri, M Adak, SK Maity, A Sarkar, S Paul, P Chakrabart Proteasome dysfunction under compromised redox metabolism dictates liver injury in NASH through ASK1/PPAR&amp;gamma binodal complementary modules Redox Biology, 2021-06-11;45(0):102043. 2021-06-11 [PMID: 34144391] (IHC, Mouse)
• Koike S, Hsu MF, Bettaieb A et al. Genetic deficiency or pharmacological inhibition of soluble epoxide hydrolase ameliorates high fat diet-induced pancreatic beta-cell dysfunction and loss Free radical biology & medicine 5/23/2021 [PMID: 34038767]
• J Misra, MJ Holmes, E T Mirek, M Langevin, HG Kim, KR Carlson, M Watford, XC Dong, TG Anthony, RC Wek Discordant regulation of eIF2 kinase GCN2 and mTORC1 during nutrient stress Nucleic Acids Research, 2021-06-04;49(10):5726-5742. 2021-06-04 [PMID: 34023907] (IHC, Mouse)
• Z Fang, HG Kim, M Huang, K Chowdhury, MO Li, S Liangpunsa, XC Dong Sestrin proteins protect against lipotoxicity-induced oxidative stress in the liver via suppression of c-Jun N-terminal kinases: Short title: Sestrins ameliorate hepatic lipotoxicity Cellular and Molecular Gastroenterology and Hepatology, 2021-05-04;0(0):. 2021-05-04 [PMID: 33962074] (IHC, Mouse)
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Review for 4-Hydroxynonenal Antibody (MAB3249) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 02/19/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 21429624
• Species: Mouse