4-Hydroxynonenal Antibody

Product Discontinued

4-Hydroxynonenal Antibody Summary

• Immunogen: 4-hydroxynonenal conjugate
• Localization: Cytoplasmic
• Marker: Lipid Peroxidation Marker
• Specificity: NB100-63093 recognises 4-hydroxynonenal (HNE). This antiserum has been shown to be immunoreactive with 4-HNE-modified bovine serum albumin (BSA) by ELISA. Additionally, this antibody has been shown by ELISA to have minimum cross-reactivity with other aldehyde-BSA conjugates, making it highly specific for 4-HNE and capable of detecting proteins modified by HNE-adducts.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:10000
  • Knockout Validated
  • Western Blot 1:3000
• Application Notes: Use in KO reported in scientific literature (PMID:32727065)Use in Immunohistochemistry reported in scientific literature (PMID 27485360). Use in DB reported in scientific literature (PMID:33516914)

Reactivity Notes

Human reactivity reported in multiple pieces of scientific literature. Mouse reactivity reported in multiple pieces of scientific literature.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for 4-Hydroxynonenal Antibody

• (2E)-4-Hydroxy-2-nonenal
• 2-Nonenal, 4-hydroxy-, (2E,4R)-
• 4 HNE
• 4Hydroxynonenal
• 4-Hydroxynonenal
• 4-hydroxy-trans-2-nonenal
• HNE

Background

4-HNE is a hydroxyalkenal which is produced by lipid peroxidation in cells. It is found in higher quantities during stress, due to the increase in the lipid peroxidation reaction that accompanies stress events. 4-Hydroxynonenanal is believed to play a key role in signal transduction in a number of pathways, including cellular adhesion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 4-Hydroxynonenal Antibody (NB100-63093)(20)

Publications using NB100-63093

• Lanzillotta C, Tramutola A, Di Giacomo G, et al. Insulin resistance, oxidative stress and mitochondrial defects in Ts65dn mice brain: a harmful synergistic path in Down syndrome Free radical biology & medicine 2021-01-28 [PMID: 33516914] (Cytometric Bead Assay Standard, Mouse)
• Zuliani I, Lanzillotta C, Tramutola A et al. The Dysregulation of OGT/OGA Cycle Mediates Tau and APP Neuropathology in Down Syndrome Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2020-11-30 [PMID: 33258073] (Mouse)
• Lanzillotta C, Zuliani I, Tramutola A et al. Chronic PERK induction promotes Alzheimer-like neuropathology in Down syndrome: Insights for therapeutic intervention Prog. Neurobiol. 2020-08-11 [PMID: 32795489] (WB, Human, Mouse)
• Lanzillotta C, Zuliani I, Vasavda C et al. BVR-A Deficiency Leads to Autophagy Impairment through the Dysregulation of AMPK/mTOR Axis in the Brain-Implications for Neurodegeneration Antioxidants (Basel) 2020-07-27 [PMID: 32727065] (WB, KO, Mouse)
• Zuliani I, Urbinati C, et al. The Anti-Diabetic Drug Metformin Rescues Aberrant Mitochondrial Activity and Restrains Oxidative Stress in a Female Mouse Model of Rett Syndrome. J Clin Med 2020-06-01 [PMID: 32492904]
• de Dios C, Bartolessis I, Roca-Agujetas V et al. Oxidative inactivation of amyloid beta-degrading proteases by cholesterol-enhanced mitochondrial stress Redox Biology 2019-09-01 [PMID: 31376793] (WB)
• Di Domenico F, Tramutola A, Barone E et al. Restoration of aberrant mTOR signaling by intranasal rapamycin reduces oxidative damage: Focus on HNE-modified proteins in a mouse model of down syndrome Redox Biol 2019-03-09 [PMID: 30876754] (WB, Mouse)
• Sharma N, Tramutola A, Lanzillotta C et al. Loss of biliverdin reductase-A favors Tau hyper-phosphorylation in Alzheimer's disease Neurobiol. Dis. 2019-05-01 [PMID: 30738142] (WB, Mouse)
• Panisello-Rosello A, Alva N, Flores M et al. Aldehyde Dehydrogenase 2 (ALDH2) in Rat Fatty Liver Cold Ischemia Injury Int J Mol Sci 2018-08-22 [PMID: 30131474] (WB, Rat)
• Barone E, Tramutola A, Triani F et al. Biliverdin Reductase-A Mediates the Beneficial Effects of Intranasal Insulin in Alzheimer Disease Mol. Neurobiol. 2018-08-02 [PMID: 30073505] (WB, Human)
• Newberry EP, Xie Y, Kennedy SM et al. Prevention of hepatic fibrosis with liver microsomal triglyceride transfer protein deletion in Liver fatty acid binding protein null mice. Hepatology. 2016-11-14 [PMID: 27862118]
• Carbonell T, Sanchez-Nuno NA, Dewey S, Gomes AV. Subnormothermic perfusion in the isolated rat liver preserves the antioxidant glutathione and enhances the function of the ubiquitin proteasome system Oxid Med Cell Longev 2016-09-15 [PMID: 27800122] (WB, Rat)
• Liu, Y, Almeida, M Et al. Skeletal inflammation and attenuation of Wnt signaling, Wnt ligand expression, and bone formation in atherosclerotic ApoE-null mice. Am J Physiol Endocrinol Metab 2016-05-01 [PMID: 26956187] (WB, Mouse)
• Ding J, Zhang Q, Luo Q et al. Alda-1 Attenuates Lung Ischemia-Reperfusion Injury by Reducing 4-Hydroxy-2-Nonenal in Alveolar Epithelial Cells Crit Care Med 2016-07-01 [PMID: 26757166] (WB, Human)
• Barone E, Di Domenico F, Cassano T et al. Impairment of biliverdin reductase-A promotes brain insulin resistance in Alzheimer disease: A new paradigm. Free Radic Biol Med 2016-02-01 [PMID: 26698666]
• Barone E, Cenini G, Di Domenico F et al. Basal brain oxidative and nitrative stress levels are finely regulated by the interplay between superoxide dismutase 2 and p53. J. Neurosci. Res. 2015-08-06 [PMID: 26251011] (Mouse)
  
  Details:
  4-Hydroxynonenal antibody was used at 1:2000 dilution for Slot-blot assay on nuclear, cytosolic or mitochondrial fractions and whole homogenates from brains of WT, SOD2 transgenic (TgSOD2), and SOD2 heterozygous knockout (SOD2?/+) mice and of mice carrying the double mutation (p53?/? × TgSOD2, p53?/? × SOD2?/+).
• Gong D, Zhang Y, Zhang H et al. Aldehyde Dehydrogenase-2 Activation during Cardioplegic Arrest Enhances the Cardioprotection against Myocardial Ischemia-Reperfusion Injury Cardiovasc Toxicol 2012-07-20 [PMID: 22814936] (WB, Rat)
• Agarwal R, Hennings L, Rafferty TM et al. Acetaminophen-induced hepatotoxicity and protein nitration in neuronal nitric-oxide synthase knockout mice J Pharmacol Exp Ther 2012-01-01 [PMID: 22001257]
• Almeida M, Ambrogini E, Han L et al. Increased Lipid Oxidation Causes Oxidative Stress, Increased Peroxisome Proliferator-activated Receptor-{gamma} Expression, and Diminished Pro-osteogenic Wnt Signaling in the Skeleton. J Biol Chem;28440:27438-27448. 2009-01-01 [PMID: 19657144]

Review for 4-Hydroxynonenal Antibody (NB100-63093) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 12/22/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 22001257
• Species: Mouse