4EBP1 Antibody (11G12C11) - BSA Free

Western Blot: 4EBP1 Antibody (11G12C11) [NBP1-47366] - Analysis using 4E-BP1 mouse mAb against truncated 4E-BP1 recombinant protein (1).

Immunohistochemistry-Paraffin: 4EBP1 Antibody (11G12C11) [NBP1-47366] - Analysis of human pancreas carcinoma (A), esophagus carcinoma tissue (B) and ovary tumor tissue, showing cytoplasmic and membrane localization using 4E-BP1 mouse mAb with DAB staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 11G12C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

4EBP1 Antibody (11G12C11) - BSA Free Summary

• Immunogen: Purified recombinant fragment of 4EBP1 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EIF4EBP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1: 500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for 4EBP1 Antibody (11G12C11) - BSA Free

• 4EBP1
• 4E-BP1
• eIF4E-binding protein 1
• EIF4EBP1
• eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1
• eukaryotic translation initiation factor 4E-binding protein 1
• PHAS-I
• PHAS-IMGC4316
• Phosphorylated heat- and acid-stable protein regulated by insulin 1

Background

4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 4EBP1 Antibody (NBP1-47366)(2)

Publications using NBP1-47366

• Li H, Liu L, Chang H et al. Downregulation of MCL-1 and upregulation of PUMA using mTOR inhibitors enhance antitumor efficacy of BH3 mimetics in triple-negative breast cancer Cell Death Dis 2018-01-26 [PMID: 29374168] (WB, Human)
• Chauhan SK, Singh VV, Rai R et al. Distinct autoantibody profiles in systemic lupus erythematosus patients are selectively associated with TLR7 and TLR9 upregulation. J Clin Immunol. 2013-07-01 [PMID: 23564191] (WB)
  
  Details:
  PBLs (WB) or B-cells (Flow intracellular) from SLE patients or controls: 1. TLR7 mAb clone IMG4G6 (IMG-6675A): WB (Figs 2a, 2c).

Bioinformatics

• Gene Symbol: EIF4EBP1
• Entrez:
  • 1978(Human)
• Uniprot:
  • Q13541()