5-hmC Antibody (13612) - Azide and BSA Free

Immunohistochemistry-Paraffin: Antibody (GT13612) - Detection of protein at nucleus on mouse testis. Dilution: 1:100

Immunohistochemistry-Paraffin: Antibody (GT13612) - Detection of protein at nucleus on mouse fore brain. Dilution 1:100

Immunohistochemistry-Paraffin: Antibody (GT13612) - Detection of protein at nucleus on mouse prostate. Dilution: 1:100

Methylated DNA Immunoprecipitation: Antibody (GT13612) - Human genomic DNA (500 ng) mixed with methylated cytosine standard kit were subjected to MeDIP at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

Dot Blot: Antibody (GT13612) - Analysis of antibody with the synthetic DNA controls. DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine.

• Reactivity: All-NA
• Applications: DB, IHC, IP, MS
• Clone: 13612
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

5-hmC Antibody (13612) - Azide and BSA Free Summary

• Immunogen: The immunogen used to generate this antibody corresponds to 5-HydroxymethylCytosine / 5-hmC
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Electron Microscopy
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
  • Methylated DNA Immunoprecipitation
• Application Notes: IHC-FR, DOT, EM, MEDIP-Assay dependent

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for 5-hmC Antibody (13612) - Azide and BSA Free

• 5'-hmC
• 5-hydroxymethylcytosine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.