![Dot Blot: 5-hmC Antibody (513) [NBP2-43568] - Analysis of anti- 5-Hydroxymethylcytosine / 5-hmC antibody [513] with DNA samples DNA samples (50 to 500 ng as indicated) were spotted onto positively charged nylon membrane and blotted with 5-Hydroxymethylcytosine / 5-hmC antibody [513] at a dilution of 1:500. A: Genomic DNA derived from 293T cells. B: Genomic DNA derived from human TET-1 transfected 293T cells.](http://images.novusbio.com/fullsize/5-hmC-Antibody-513-Dot-Blot-NBP2-43568-img0001.jpg "Dot Blot: 5-hmC Antibody (513) [NBP2-43568] - Analysis of anti- 5-Hydroxymethylcytosine / 5-hmC antibody [513] with DNA samples DNA samples (50 to 500 ng as indicated) were spotted onto positively charged nylon membrane and blotted with 5-Hydroxymethylcytosine / 5-hmC antibody [513] at a dilution of 1:500. A: Genomic DNA derived from 293T cells. B: Genomic DNA derived from human TET-1 transfected 293T cells.")
| Reactivity | All-NASpecies Glossary |
| Applications | DB, IP |
| Clone | 513 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | The immunogen used to generate this antibody corresponds to 5-hmC |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Dot Blot: 5-hmC Antibody (513) [NBP2-43568] - Analysis of anti-5-hmC antibody with the synthetic DNA controls . DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine.](http://images.novusbio.com/fullsize/5-hmC-Antibody-513-Dot-Blot-NBP2-43568-img0002.jpg "Dot Blot: 5-hmC Antibody (513) [NBP2-43568] - Analysis of anti-5-hmC antibody with the synthetic DNA controls . DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine.")
![Methylated DNA Immunoprecipitation: 5-hmC Antibody (513) [NBP2-43568] - Analysis of Human genomic DNA (500 ng) mixed with methylated cytosine standard kit, using 5-Hydroxymethylcytosine/ 5-hmC antibody [513] at 1:500 dilution. Analysized with semi-quantitative PCR and Immunprecipitated DNA was plotted as % of input DNA.](http://images.novusbio.com/fullsize/5-hmC-Antibody-513-Methylated-DNA-Immunoprecipitation-NBP2-43568-img0004.jpg "Methylated DNA Immunoprecipitation: 5-hmC Antibody (513) [NBP2-43568] - Analysis of Human genomic DNA (500 ng) mixed with methylated cytosine standard kit, using 5-Hydroxymethylcytosine/ 5-hmC antibody [513] at 1:500 dilution. Analysized with semi-quantitative PCR and Immunprecipitated DNA was plotted as % of input DNA.")
