6 Phosphofructo 2 Kinase Antibody (JM43-43)

Western Blot: 6 Phosphofructo 2 Kinase Antibody (JM43-43) [NBP2-75393] - All lanes: Western blot analysis of PFKFB3 with anti-PFKFB3 antibody [JM43-43] at 1:500 dilution. Lane 1: Wild-type Hela whole cell lysate. Lane 2: PFKFB3 knockout Hela whole cell lysate. was shown to specifically react with PFKFB3 in wild-type Hela cells. No band was observed when PFKFB3 knockout samples were tested. Wild-type and PFKFB3 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-PFKFB3 antibody (1/500) and Anti-HSP90 antibody (1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: 6 Phosphofructo 2 Kinase Antibody (JM43-43) [NBP2-75393] - Staining PFKFB3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: 6 Phosphofructo 2 Kinase Antibody (JM43-43) [NBP2-75393] - Analysis of paraffin-embedded rat kidney tissue using anti-PFKFB3 antibody. Counter stained with hematoxylin.

Flow Cytometry: 6 Phosphofructo 2 Kinase Antibody (JM43-43) [NBP2-75393] - Analysis of A431 cells with PFKFB3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Western Blot: 6 Phosphofructo 2 Kinase Antibody (JM43-43) [NBP2-75393] - Western blot analysis of 6 Phosphofructo 2 Kinase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: PC-12 cell lysate Lane 2: A431 cell lysate

Immunocytochemistry/Immunofluorescence: 6 Phosphofructo 2 Kinase Antibody (JM43-43) [NBP2-75393] - Staining PFKFB3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: 6 Phosphofructo 2 Kinase Antibody (JM43-43) [NBP2-75393] - Analysis of paraffin-embedded mouse colon tissue using anti-PFKFB3 antibody. Counter stained with hematoxylin.

Product Discontinued

6 Phosphofructo 2 Kinase Antibody (JM43-43) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human 6 Phosphofructo 2 Kinase aa 260-520 / 520. (SwissProt: Q16875 Human; SwissProt: O35552 Rat; SwissProt: Q3U3S6 Mouse)
• Localization: Cytosol. Nucleoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PFKFB3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for 6 Phosphofructo 2 Kinase Antibody (JM43-43)

• 6 Phosphofructo 2 Kinase
• 6PF-2-K/Fru-2,6-P2ase 3,6-bisphosphatase
• 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase
• 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3,6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
• FLJ37326
• inducible 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
• IPFK2
• PFK/FBPase 3
• PFK2
• PFKFB3
• Renal carcinoma antigen NY-REN-56

Background

Synthesis and degradation of fructose 2,6-bisphosphate

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PFKFB3