ELISA: 8-OHdG Antibody [NB600-1508] - Competitive ELISA using the 8-OHG antibody demonstrates a high specificity towards 8-hydroxydeoxyguanosine (8-OHdG) and 8-hydroxyguanosine nucleotides. Partial cross-reactivity is ...read more
For IHC-Paraffin: It is suggested that the tissues are pretreated with 10 ug/mL of proteinase K for 40 minutes at 37 degrees C prior to staining. Dewaxed sections treated with 3%H2O2 in methanol for 30 minutes and blocking was with 10% serum in TBS. Sections were treated with proteinase K 10ug/ml in PBS pH 7.4, 40 minutes 37 degrees C prior to blocking. Detection is via DAB. Since the antibody is staining a nucleic acid it is important to use enough proteinase to penetrate the histone barrier.
Use in ICC/IF reported in scientific literature (PMID: 28269997). Use in Western Blot reported in scientific literature (PMID:32269708).
Publications
Read Publications using NB600-1508 in the following applications:
Due to the conserved nature of the target cross reactivity would be expected in wide range of species. Mouse reactivity reported in scientific literature (PMID: 29559852).
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.01% Thimerosal
Purity
Unpurified
Alternate Names for 8-oxo-dG Antibody
8-Hydroxyguanine
8-OHG
8oxodG
8-oxo-dG
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for 8-OHdG Antibody (NB600-1508)(17)
We have publications tested in 3 confirmed species: Human, Mouse, Canine.
We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-P, WB.
Tang L, Xie L, Zhou H et al. Perillaldehyde mitigates ionizing radiation-induced intestinal injury by inhibiting ferroptosis via the Nrf2 signaling pathway Research Square 2023-03-06 (IHC-P)
FAQs for 8-OHdG Antibody (NB600-1508). (Showing 1 - 1 of 1 FAQs).
NB600-1508 could be applied on mouse tissue?
Since 8-hydroxyguanosine is a modified base which occurs in DNA as a result of oxidative stress, hence considered a sensitive marker of oxidative damage in cellular DNA, its structure is pretty conserved among different species. Therefore, the cross reactivity of this antibody may be expected in wide range of species. It is worth mentioning NB600-1508 does not cross react with other naturally occurring nucleotides.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
=
÷
Review this Product
Be the first to review our 8-oxo-dG Antibody and receive a gift card or discount.
![ELISA: 8-OHdG Antibody [NB600-1508] - Competitive ELISA using the 8-OHG antibody demonstrates a high specificity towards 8-hydroxydeoxyguanosine (8-OHdG) and 8-hydroxyguanosine nucleotides. Partial cross-reactivity is observed with 8-mercaptoguanosine while 8-bromoguanosine shows some cross-reactivity. 2-Deoxyadenosine, 7-methylguanosine, guanosine monophosphate and guanosine show no reactivity.](http://images.novusbio.com/fullsize/8-OHdG-Antibody-ELISA-NB600-1508-img0008.jpg "ELISA: 8-OHdG Antibody [NB600-1508] - Competitive ELISA using the 8-OHG antibody demonstrates a high specificity towards 8-hydroxydeoxyguanosine (8-OHdG) and 8-hydroxyguanosine nucleotides. Partial cross-reactivity is observed with 8-mercaptoguanosine while 8-bromoguanosine shows some cross-reactivity. 2-Deoxyadenosine, 7-methylguanosine, guanosine monophosphate and guanosine show no reactivity.")
