8-oxo-dG Antibody

ELISA: 8-OHdG Antibody [NB600-1508] - Competitive ELISA using the 8-OHG antibody demonstrates a high specificity towards 8-hydroxydeoxyguanosine (8-OHdG) and 8-hydroxyguanosine nucleotides. Partial cross-reactivity is observed with 8-mercaptoguanosine while 8-bromoguanosine shows some cross-reactivity. 2-Deoxyadenosine, 7-methylguanosine, guanosine monophosphate and guanosine show no reactivity.

• Reactivity: Hu, Mu
• Applications: ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

8-oxo-dG Antibody Summary

• Immunogen: 8-Hydroxy-2'-deoxyguanosine and 8-hydroxyguanosine.
• Localization: Nuclear
• Specificity: NB600-1508 cross reacts completely with 8-oxo-dG, and does not cross react with other naturally occurring nucleotides.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100000-1:250000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
• Application Notes: For IHC-Paraffin: It is suggested that the tissues are pretreated with 10 ug/mL of proteinase K for 40 minutes at 37 degrees C prior to staining. Dewaxed sections treated with 3%H2O2 in methanol for 30 minutes and blocking was with 10% serum in TBS. Sections were treated with proteinase K 10ug/ml in PBS pH 7.4, 40 minutes 37 degrees C prior to blocking. Detection is via DAB. Since the antibody is staining a nucleic acid it is important to use enough proteinase to penetrate the histone barrier.
  
  Use in ICC/IF reported in scientific literature (PMID: 28269997).
  Use in Western Blot reported in scientific literature (PMID:32269708).

Reactivity Notes

Due to the conserved nature of the target cross reactivity would be expected in wide range of species. Mouse reactivity reported in scientific literature (PMID: 29559852).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.01% Thimerosal
• Purity: Unpurified

Alternate Names for 8-oxo-dG Antibody

• 8-Hydroxyguanine
• 8-OHG
• 8oxodG
• 8-oxo-dG

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for 8-OHdG Antibody (NB600-1508)(19)

Publications using NB600-1508

• Tang L, Xie L, Zhou H et al. Perillaldehyde mitigates ionizing radiation-induced intestinal injury by inhibiting ferroptosis via the Nrf2 signaling pathway Research Square 2023-03-06 (IHC-P)
• Lin-Feng Tang, Xiaoming Ma, Li-Wei Xie, Hao Zhou, Jiahua Yu, Zhen-Xin Wang, Ming Li Perillaldehyde Mitigates Ionizing Radiation-Induced Intestinal Injury by Inhibiting Ferroptosis via the Nrf2 Signaling Pathway. Molecular nutrition & food research 2023-10-01 [PMID: 37658487]
• Huang J, Bai Y, Xie W et al. Lyciumbarbarum polysaccharides ameliorate canine acute liver injury by reducing oxidative stress, protecting mitochondrial function, and regulating metabolic pathways Journal of Zhejiang University. Science. B 2023-02-15 [PMID: 36751701] (Canine)
• M Denechaud, S Geurs, T Comptdaer, S Bégard, A Garcia-Núñ, LA Pechereau, T Bouillet, Y Vermeiren, PP De Deyn, R Perbet, V Deramecour, CA Maurage, M Vanderhaeg, S Vanuytven, B Lefebvre, E Bogaert, N Déglon, T Voet, M Colin, L Buée, B Dermaut, MC Galas Tau promotes oxidative stress-associated cycling neurons in S phase as a pro-survival mechanism: possible implication for Alzheimer's disease Progress in neurobiology, 2022-12-06;0(0):102386. 2022-12-06 [PMID: 36481386]
• Davis CK, Bathula S, Hsu M et al. An antioxidant and anti-ER stress combo therapy decreases inflammation, secondary brain damage and promotes neurological recovery following traumatic brain injury in mice The Journal of neuroscience : the official journal of the Society for Neuroscience 2022-07-25 [PMID: 35882557]
• Xiao Y, Yao W, Lin M et al. Icaritin-loaded PLGA nanoparticles activate immunogenic cell death and facilitate tumor recruitment in mice with gastric cancer Drug Delivery 2022-12-31 [PMID: 35635307]
• Toomey LM, Papini M, Lins B Et al. Cuprizone feed formulation influences the extent of demyelinating disease pathology Scientific reports 2021-11-19 [PMID: 34799634] (IF/IHC, Mouse)
• Feng J, Li Y, Jin X Et al. ATF3 regulates oxidative stress and extracellular matrix degradation via p38/Nrf2 signaling pathway in pelvic organ prolapse Tissue & cell 2021-10-02 [PMID: 34666282] (ICC/IF, Human)
• M Cui, A Atmanli, MG Morales, W Tan, K Chen, X Xiao, L Xu, N Liu, R Bassel-Dub, EN Olson Nrf1 promotes heart regeneration and repair by regulating proteostasis and redox balance Nature Communications, 2021-09-06;12(1):5270. 2021-09-06 [PMID: 34489413]
• Ho YS, Lau CF, Lee K, et al. Impact of unilateral ureteral obstruction on cognition and neurodegeneration Brain research bulletin 2021-01-07 [PMID: 33422661] (IF/IHC)
• Dai E, Han L, Liu J et al. Ferroptotic damage promotes pancreatic tumorigenesis through a TMEM173/STING-dependent DNA sensor pathway Nature communications 2020-12-11 [PMID: 33311482] (ICC/IF, Mouse)
• Villarreal-Ponce A, Tiruneh MW, Lee J et al. Keratinocyte-Macrophage Crosstalk by the Nrf2/Ccl2/EGF Signaling Axis Orchestrates Tissue Repair Cell Rep 2020-11-24 [PMID: 33238115]
• Rahman H, Kumar D, Liu T et al. Aspirin protects melanocytes and keratinocytes against UVB-induced DNA damage in vivo J. Invest. Dermatol. 2020-06-19 [PMID: 32569596]
• Zhou X, Wang Z, Ni Y et al. Suppression effect of N-acetylcysteine on bone loss in ovariectomized mice Am J Transl Res Mar 15 2020 [PMID: 32269708] (WB, Mouse)
• Prieto, I;Zambrano, A;Laso, J;Aranda, A;Samper, E;Monsalve, M; Early induction of senescence and immortalization in PGC-1 alpha-deficient mouse embryonic fibroblasts Free Radic. Biol. Med. 2019-04-25 [PMID: 31029787]
• Strowitzki MJ, Radhakrishnan P, Pavicevic S et al. High hepatic expression of PDK4 improves survival upon multimodal treatment of colorectal liver metastases Br. J. Cancer 2019-02-27 [PMID: 30808993] (IHC-P, Human)
• Dai X, Zhang Q, Yu Z et al. Bmi1 Deficient Mice Exhibit Male Infertility Int. J. Biol. Sci. 2018-03-09 [PMID: 29559852] (IHC-P, Mouse)
• Sanchez Ramos C, Prieto I, Tierrez A et al. PGC-1a downregulation in the steatotic liver enhances ischemia-reperfusion injury and impairs ischemic preconditioning. Antioxid. Redox Signal. 2017-03-07 [PMID: 28269997] (ICC/IF, Human)
• Alberto Zambrano, Verónica García-Carpizo, María Esther Gallardo, Raquel Villamuera, Maria Ana Gómez-Ferrería, Angel Pascual, Nicolas Buisine, Laurent M. Sachs, Rafael Garesse, Ana Aranda The thyroid hormone receptor beta induces DNA damage and premature senescence The Journal of Cell Biology 2014-01-06 [PMID: 24395638]

FAQs for 8-OHdG Antibody (NB600-1508). (Showing 1 - 1 of 1 FAQs).

1. NB600-1508 could be applied on mouse tissue?
   • Since 8-hydroxyguanosine is a modified base which occurs in DNA as a result of oxidative stress, hence considered a sensitive marker of oxidative damage in cellular DNA, its structure is pretty conserved among different species. Therefore, the cross reactivity of this antibody may be expected in wide range of species. It is worth mentioning NB600-1508 does not cross react with other naturally occurring nucleotides.