ABpure™ Antibody Purification Kit

ABpure Antibody Purification Kit [260-0010] - SDS-PAGE shows the sample containing Rabbit IgG pre and post purification by using AbPure Purification kit. BSA has been successfully removed without compromising the antibody recovery.

Product Discontinued

ABpure™ Antibody Purification Kit Summary

• Description: Commercially available antibodies often contain substances (e.g. BSA and other proteins, glycine, tris, and azide) that interfere in conjugation reactions. The AbPure Antibody Purification System quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up ideally is in a volume of 0.1ml to 0.5ml. 20 to 500ug of antibody can be purified in each run.
  
  

  Binding Affinity

  Species	Isotype	Binding Strength
  Rabbit	IgG	High
  Human	IgG	High
  Pig	IgG	High
  Mouse	IgG1	Low/Medium
  Mouse	IgG2a	High
  Mouse	IgG2b	High
  Mouse	IgG3	Low/Medium
  Goat	IgG	Low
  Sheep	IgG	Low
  Rat	IgG	Low

• Kit Type: Antibody Purification Kit

Applications/Dilutions

• Application Notes: The volume of antibody to be purified or cleaned up should be 0.1 - 0.5 mL, though larger volumes may be processed by first incubating the antibody sample (combined with the 10X AbPure Binding Buffer) with the Protein A resin in a larger vessel (e.g. 2 mL microcentrifuge tube) prior to transferring to the spin cartridge in several aliquots, spinning down excess liquid each time.

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Notes

This prodcut is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.