AbSelect™ Mouse TCS Antibody Purification Kit Summary
Description |
The AbSelect Mouse TCS Purification System enables the selective purification of mouse IgG from hybridoma supernatants without the co-purification of bovine IgG. This is because AbSelect Mouse resin has a high affinity and specificity for mouse IgG molecules whereas the binding strength of bovine IgG to AbSelect Mouse resin is negligible. In comparison, Protein G does not have the capability to selectively purify mouse IgG from hybridoma supernatants.
The method of AbSelect Mouse TCS Purification System involves capture of the antibody on the AbSelect Mouse resin and the removal of unwanted substances using a simple wash procedure. The purified antibody is then eluted and neutralized. AbSelect Mouse TCS Purification System is not suitable for use with antibodies from other species.
The components of all AbSelect kits are fully compatible with the Lightning-Link and Lightning-Link Rapid antibody labeling systems (available separately), which allow the purified antibody to be rapidly conjugated to a wide variety of labels. |
Kit Type |
Antibody Purification Kit |
Applications/Dilutions
Application Notes |
The mouse antibody to be purified should be in 10 to 25ml of tissue culture supernatant. Up to 1.5mg of antibody can be purified in each run. |
Packaging, Storage & Formulations
Kit Components
Components
|
- 1 or 3 spin cartridge / collecting tube assemblies
- 1 or 3 vials of AbSelect Mouse resin
- 1 vial of 10x Binding buffer
- 1 vial of Elution buffer
- 1 vial of Neutralization buffer
- 1 vial of Washing buffer
- 4 or 12 additional collecting tubes
|
Notes
AbSelect® is a registered trademark of Abcam and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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