Acetyl-Lysine Antibody (7F8) - BSA Free

Western Blot: Acetyl-Lysine Antibody (7F8) [NB110-96876] - Analysis of Acetyl-Lysine showing detection of Acetyl-Lysine protein using Mouse Anti-Acetyl-Lysine Monoclonal Antibody, Clone 7F8 . Primary Antibody: Mouse Anti-Acetyl-Lysine Monoclonal Antibody at 1:1000. (1) acetylated BSA (75ng of protein), (2) non-acetylated BSA, and (3) marker.

Immunohistochemistry: Acetyl-Lysine Antibody (7F8) [NB110-96876] - Immunohistochemistry analysis using Mouse Anti-Acetyl-LysineMonoclonal Antibody, Clone 7F8 (NB110-96876). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Acetyl-LysineMonoclonal Antibody (NB110-96876) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.

Western Blot: Mouse Monoclonal Acetyl-Lysine Antibody (7F8) [NB110-96876] - Analysis using mouse liver lysates. Antibody concentration used was 1:1000, diluted in 1x TBST. Image from a verified customer review.

• Reactivity: All-NA
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 7F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Acetyl-Lysine Antibody (7F8) - BSA Free Summary

• Immunogen: Acetylated KLH
• Localization: Nucleus
• Specificity: Detects proteins containing acetylated lysine residues in ELISA and western blots. Does not detect non-acetylated lysine residues.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
  • Western Blot 1:1000
• Application Notes: 1 ug of Acetyl-Lysine Antibody was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 ug total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Acetyl-Lysine Antibody (7F8) - BSA Free

• acetyl Lysine
• AC-LYS-OH
• Lysine
• N-acetyl lysine

Background

Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Ne development (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Acetyl-Lysine Antibody (NB110-96876)(7)

Publications using NB110-96876

• Stojnova K, Racheva P, Divarova V et al. Study on the Complex Equilibria of Molybdenum(VI) with 3,5-Dinitrocatechol and Ditetrazolium Salt. Acta chimica Slovenica 2017-07-13 [PMID: 27640393]
• Lazi? I, Bosch EGT, Lazar S. Phase contrast STEM for thin samples: Integrated differential phase contrast. Ultramicroscopy 2016-04-15 [PMID: 26590505]
• 1 Yang XJ. Oncogene. 24:1653-1662. 2005-01-01 [PMID: 15744326]
• 3 Yang XJ. Bioessays 26:1076-1087. 2004-01-01 [PMID: 15382140]
• 5 Vigushin, DM Coombes, RC. Curr. Cancer Drug Targets 4: 205-218. 2004-01-01 [PMID: 15032670]
• 4 Hughes, RE. Curr. Biol. 12: R141-R143. 2002-01-01 [PMID: 11864588]
• 2 Hassig, CA Schreiber, SL. Curr. Opin. Chem. Biol. 1(3): 300-308. 1997-01-01 [PMID: 9667866]