Acetylcholinesterase/ACHE Antibody

Western Blot: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Staining (0.3ug/ml) of Jurkat (A) and (0.5ug/ml) HepG2 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear, membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Flow Cytometry: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Acetylcholinesterase/ACHE Antibody Summary

• Immunogen: Peptide with sequence QFDHYSKQDRCSDL corresponding to C-Terminus according to NP_000656.1.
• Marker: Early Neuronal Development Marker
• Specificity: This antibody is expected to recognise isoform NP_000656.1 only (the ubiquitously expressed, hydrophillic form).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACHE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry Free-Floating
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: This Acetylcholinesterase/ACHE antibody is validated for IHC-FrFl from a verified customer review.
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)
  Acetylcholinesterase/ACHE Overexpression Lysate

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Acetylcholinesterase/ACHE Antibody

• acetylcholinesterase (Yt blood group)
• Acetylcholinesterase
• ACHE
• apoptosis-related acetylcholinesterase
• ARACHE
• EC 3.1.1
• EC 3.1.1.7
• N-ACHE
• Yt blood group
• YT

Background

Acetylcholinesterase, or ACHE, hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Acetylcholinesterase/ACHE Antibody (NB100-1519)(1)

Publication using NB100-1519

• Cottingham MG, Voskuil JL, Vaux DJ. The intact human acetylcholinesterase C-terminal oligomerization domain is alpha-helical in situ and in isolation, but a shorter fragment forms beta-sheet-rich amyloid fibrils and protofibrillar oligomers. Biochemistry 2003-09-16 [PMID: 12962511]

Review for Acetylcholinesterase/ACHE Antibody (NB100-1519) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-Floating section Mouse 11/21/2016

Summary

• Application: Other
• Sample Tested: Mouse brain
• Species: Mouse

Comments

• Comments: Floating sections were incubated overnight at 4C with a 1:200 dilution of the above ACHE antibody, washed and then incubated for 1 hour at room temperature with a 1:500 dilution of donkey anti-rabbit DyLight 594 secondary antibody (Cat#NBP1-75637).

Bioinformatics

• Gene Symbol: ACHE
• Entrez:
  • 43(Human)
  • 11423(Mouse)
• Uniprot:
  • NP_000656.(Human)