ACF1 Antibody

Western Blot: ACF1 Antibody [NB100-61042] - Detection of Human ACF1/BAZ1A on HeLa whole cell lysates using NB100-61042. ACF1/BAZ1A was also immunoprecipitated by rabbit anti-ACF1/BAZ1A antibody NB100-61041.

Product Discontinued

ACF1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1506 and 1556 of human ATP-dependent chromatin remodeling protein 1 (Bromodomain adjacent to zinc finger domain protein 1A ) using the numbering given in entry NP_038476.2
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BAZ1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 178 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ACF1 Antibody

• ACF1hWALp1
• ATP-dependent chromatin remodeling protein
• ATP-dependent chromatin-remodeling protein
• ATP-utilizing chromatin assembly and remodeling factor 1
• bromodomain adjacent to zinc finger domain protein 1A
• bromodomain adjacent to zinc finger domain, 1A
• CHRAC subunit ACF1
• hACF1FLJ14383
• WALp1
• WCRF180DKFZp586E0518
• Williams syndrome transcription factor-related chromatin-remodeling factor 180

Background

ACF1/BAZ1A is a subunit of the ACF chromatin-remodeling complex and the ISWI CHRAC remodeling complex. In the ACF complex, ACF1/BAZ1A functions to influence the nucleosome remodeling activity of the SNF2h ATPase. As part of the CHRAC complex, ACF1/BAZ1A may function to target CHRAC to heterochromatin. ACF1 has also been shown to interact with the nuclear receptor corepressor protein (N-CoR) to facilitate the repression of transcription by unliganded nuclear receptors. Alternate names for ACF1/BAZ1A include ATP-utilizing chromatin assembly and remodeling factor1, hACF1, bromodomain adjacent to zinc finger domain protein 1A, Williams syndrome transcription factor-related chromatin-remodeling factor 180, WCRF180, hWALp1, and CHRAC subunit ACF1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACF1 Antibody (NB100-61042)(2)

Publications using NB100-61042

• Inturi R, Mun K, et al. Human Adenovirus Infection Causes Cellular E3 Ubiquitin Ligase MKRN1 Degradation Involving the Viral Core Protein pVII. J Virol 2018-02-01 [PMID: 29142133] (PLA, Human)
• Oppikofer M, Bai T, et al. Expansion of the ISWI chromatin remodeler family with new active complexes. EMBO Rep 2017-10-01 [PMID: 28801535] (IP, Human)

Bioinformatics

• Gene Symbol: BAZ1A
• Entrez:
  • 11177(Human)
• Uniprot:
  • Q9NRL2(Human)