ACOX2 Antibody

Western Blot: ACOX2 Antibody [NB100-57853] - (0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Rb
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ACOX2 Antibody Summary

• Immunogen: Peptide with sequence C-QWAQKSPTNTQENP corresponding to internal region (near C-Terminus) according to NP_003491.1.
• Predicted Species: Rabbit (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACOX2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in human liver lysates (calculated MW of 76.8 kDa band according to NP_003491.1). An additional band of 37 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than WB.
• Theoretical MW: 77 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ACOX2 Antibody

• 12-alpha-trihydroxy-5-beta-cholestanoyl-CoA 24-hydroxylase
• 12-alpha-trihydroxy-5-beta-cholestanoyl-CoA oxidase
• 3-alpha
• 7-alpha
• acyl-CoA oxidase 2, branched chain
• acyl-Coenzyme A oxidase 2, branched chain
• BCOX
• BRCACOXTHCA-CoA oxidase
• BRCOX
• EC 1.17.99.3
• peroxisomal acyl-coenzyme A oxidase 2,3-alpha
• peroxisomal branched chain acyl-CoA oxidase
• THCCox
• Trihydroxycoprostanoyl-CoA oxidase

Background

The product of this gene belongs to the acyl-CoA oxidases family. It encodes the branched-chain acyl-CoA oxidase which is involved in the degradation of long branched fatty acids and bile acid intermediates in peroxisomes. Deficiency of this enzyme results in the accumulation of branched fatty acids and bile acid intermediates, and may lead to Zellweger syndrome, severe mental retardation and death in children.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACOX2 Antibody (NB100-57853)(1)

Publication using NB100-57853

• Baumgart E, Vanhooren JC, Fransen M et al. Molecular characterization of the human peroxisomal branched-chain acyl-CoA oxidase: cDNA cloning, chromosomal assignment, tissue distribution, and evidence for the absence of the protein in Zellweger syndrome. Proc Natl Acad Sci U S A 1996-11-26 [PMID: 8943006]

Bioinformatics

• Gene Symbol: ACOX2
• Entrez:
  • 8309(Human)
• Uniprot:
  • NP_003491.1(Human)