Activin RIIA/B Antibody (60401)

• Reactivity: Hu
• Applications: WB
• Clone: 60401
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Activin RIIA/B Antibody (60401) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Activin RIIB
  Ser19-Thr134
  Accession # Q13705
• Specificity: Detects human Activin RII in direct ELISA and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACVR2A
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Activin RIIA/B Antibody (60401)

• Activin RIIA/B
• ACTRII
• ACVR2

Background

Members of the TGF- beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor-beta (TGF-beta) family members, including Activin, Nodal, Bmp7, GDF1, GDF3, Myostatin (GDF8), and GDF11. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Activin RIIA/B Antibody (MAB3391)(2)

Publications using MAB3391

• Yang L, Geng Z, Nickel T, Johnson C, Gao L, Dutton J, Hou C, Zhang J Differentiation of Human Induced-Pluripotent Stem Cells into Smooth-Muscle Cells: Two Novel Protocols. PLoS ONE, 2016-01-15;11(1):e0147155. 1/15/2016 [PMID: 26771193]
• Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7. J. Mol. Biol., 2008-07-02;381(4):1025-39. 2008-07-02 [PMID: 18621057] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: ACVR2A
• Uniprot:
  • Q13705()