ADAM10 Antibody (139712) [Fluorescein]

Mouse splenocytes were stained with Rat Anti-Mouse ADAM10 Ectodomain Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB946F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 139712
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Fluorescein

ADAM10 Antibody (139712) [Fluorescein] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ADAM10
  Thr215-Glu673
  Accession # O35598
• Specificity: Detects mouse ADAM10 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) rhADAM10 is observed and no cross-reactivity with rhADAM8, recombinant mouse (rm) ADAM9, rmADAM15, rhBACE, or rhTACE is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: ADAM10
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM10 Antibody (139712) [Fluorescein]

• a disintegrin and metalloprotease domain 10
• a disintegrin and metalloproteinase domain 10
• AD10
• ADAM 10
• ADAM metallopeptidase domain 10
• ADAM10
• CD156c antigen
• CD156c
• CDw156
• disintegrin and metalloproteinase domain-containing protein 10
• EC 3.4.24
• HsT18717
• kuz
• Kuzbanian protein homolog
• Kuzbanian
• MADM
• MADMEC 3.4.24.81
• Mammalian disintegrin-metalloprotease

Background

ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).

1. Rooke, J. et al. (1996) Science 273:1227.
2. Pan, D. and Rubin, G.M. (1997) Cell 90:271.
3. Qi, H. et al. (1999) Science 283:91.
4. Lammich, S. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
5. Rosendahl, M.S. et al. (1997) J. Biol. Chem. 272:24588.
6. Chantry, A. et al. (1989) J. Biol. Chem. 264:21603.
7. Millichip, M.I. et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
8. Chantry, A. and Glynn, P. (1990) Biochem. J. 268:245.
9. Fahrenholz, F.S. et al. (2000) Ann. N.Y. Acad. Sci. 920:215.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAM10 Antibody (FAB946F)(2)

Publications using FAB946F

• Padro C, Shawler T, Gormley M, Sanders V Adrenergic regulation of IgE involves modulation of CD23 and ADAM10 expression on exosomes. J Immunol, 2013-10-18;191(11):5383-97. 2013-10-18 [PMID: 24140643] (Flow Cytometry, Mouse)
• Gibb DR, Saleem SJ, Kang DJ, Subler MA, Conrad DH ADAM10 Overexpression Shifts Lympho- and Myelopoiesis by Dysregulating Site 2/Site 3 Cleavage Products of Notch. J. Immunol., 2011-03-02;186(7):4244-52. 2011-03-02 [PMID: 21368228] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: ADAM10
• Uniprot:
  • O35598()