ADAM33 Antibody (508101)

A549 human lung carcinoma cell line was stained with Mouse Anti-Human ADAM33 Monoclonal Antibody (Catalog # MAB5565, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: IP, CyTOF-ready, Flow
• Clone: 508101
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ADAM33 Antibody (508101) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ADAM33
  Val30-Leu501 (predicted)
  Accession # Q9BZ11
• Specificity: Detects human ADAM33 in direct ELISAs. In direct ELISAs, less than 5% cross‑reactivity with recombinant mouse ADAM33 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADAM33
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunoprecipitation 25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM33 Antibody (508101)

• a disintegrin and metalloprotease 33
• a disintegrin and metalloproteinase domain 33
• ADAM 33
• ADAM metallopeptidase domain 33
• ADAM33
• C20orf153
• chromosome 20 open reading frame 153
• disintegrin and metalloproteinase domain-containing protein 33
• disintegrin and reprolysin metalloproteinase family protein
• dJ964F7.1
• DKFZp434K0521
• EC 3.4.24.-
• FLJ35308
• FLJ36751
• MGC149823
• MGC71889

Background

ADAM33 is a widely expressed member of the ADAM family of transmembrane proteins. It contains a metalloprotease-like domain, disintegrin cell adhesion domain, and cysteine rich domain. ADAM33 is involved in the maintenance of airway function, and ADAM33 polymorphisms have been associated with asthma susceptibility and bronchial hyperresponsiveness. Within the mature ectodomain, human and mouse ADAM33 share 79% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADAM33
• Uniprot:
  • Q9BZ11()