Adenine Nucleotide Translocator 2 Antibody - Azide and BSA Free

Western Blot: Adenine Nucleotide Translocator 2 Antibody [H00000292-B01P] - Analysis of SLC25A5 expression in human liver.

Western Blot: Adenine Nucleotide Translocator 2 Antibody [H00000292-B01P] - Analysis of SLC25A5 expression in transfected 293T cell line by SLC25A5 polyclonal antibody. Lane 1: SLC25A5 transfected lysate(32.89 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Adenine Nucleotide Translocator 2 Antibody - Azide and BSA Free Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: SLC25A5 (AAH56160, 1 a.a. - 298 a.a.) full-length human protein. MTDAAVSFAKDFLAGGVAAAISKTAVAPIERVKLLLQVQHASKQITADKQYKGIIDCVVRIPKEQGVLSFWRGNLANVIRYFPTQALNFAFKDKYKQIFLGGVDKRTQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKAGAEREFRGLGDCLVKIYKSDGIKGLYQGFNVSVQGIIIYRAAYFGIYDTAKGMLPDPKNTHIVISWMIAQTVTAVAGLTSYPFDTVRRRMMMQSGRKGTDIMYTGTLDCWRKIARDEGGKAFFKGAWSNVLRGMGGAFVLVLYDEIKKYT
• Specificity: SLC25A5 - solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: SLC25A5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Adenine Nucleotide Translocator 2 Antibody - Azide and BSA Free

• adenine nucleotide translocator 2 (fibroblast)
• Adenine nucleotide translocator 2
• ADP
• ADP/ATP carrier protein
• ADP/ATP translocase 2
• ANT2AAC2, 2F1
• ATP carrier protein 2
• ATP carrier protein, fibroblast isoform
• solute carrier family 25 (mitochondrial carrier; adenine nucleotidetranslocator), member 5
• Solute carrier family 25 member 5
• T2
• T3ANT 2

Background

ADP/ATP translocase, the most abundant mitochondrial protein, is an integral component of the inner mitochondrial membrane. It facilitates exchange of ADP and ATP between the cytosol and the mitochondria, thereby linking the subcellular compartment of ATP production to those of ATP utilization. SLC25A5 is 1 of at least 3 transcriptionally active ADP/ATP translocase genes in humans (Chen et al., 1990 [PubMed 2157297]). Battini et al. (1987) [PubMed 3031073] cloned an ADP/ATP translocase gene from an Okayama-Berg library derived from SV40-transformed human fibroblasts. Ku et al. (1990) [PubMed 2168878] cloned and sequenced the ANT2 gene. Chen et al. (1990) [PubMed 2157297] isolated 7 ADP/ATP translocase pseudogenes from recombinant human genomic libraries. Each pseudogene sequence had more than 85% identity with the sequence of the translocase cDNA derived from fibroblast mRNA, but each had mutations that precluded synthesis of a functional protein.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Adenine Nucleotide Translocator 2 Antibody (H00000292-B01P)(3)

Publications using H00000292-B01P

• Watanabe M, Yamada Y, Kurumida Y et al. Rabdosianone I, a Bitter Diterpene from an Oriental Herb, Suppresses Thymidylate Synthase Expression by Directly Binding to ANT2 and PHB2 Cancers 2021-02-26 [PMID: 33652782] (WB, Human)
• Ma?ecki JM, Willemen HLDM, Pinto R et al. Lysine methylation by the mitochondrial methyltransferase FAM173B optimizes the function of mitochondrial ATP synthase. J Biol Chem 2018-12-10 [PMID: 30530489]
• Watanabe M, Iizumi Y, Sukeno M et al. The pleiotropic regulation of cyclin D1 by newly identified sesaminol-binding protein ANT2. Oncogenesis 2017-04-03 [PMID: 28368390]

Bioinformatics

• Gene Symbol: SLC25A5
• Entrez:
  • 292(Human)
• Uniprot:
  • AAH56160(Human)