Adenovirus Antibody (8C4) - BSA Free

• Reactivity: V, V-Vi
• Applications: ELISA, Func, ICC/IF, IHC, LFA
• Clone: 8C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Adenovirus Antibody (8C4) - BSA Free Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Human adenovirus type 1 and canine adenovirus type 1.
• Specificity: Hexon antigen of human adenovirus
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunodiffusion
  • Immunohistochemistry 1:10-1:500
  • Lateral Flow Assay
  • Sandwich ELISA 1:100-1:2000
• Application Notes: Recommended pair for sandwich ELISA (capture-detection): NB600-413 - NB200-425. This antibody has been reported in the literature to work in Immunohistochemistry-Frozen sections (PMID: 19622582). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 26861248). Use in Block/Neutralize reported in scientific literature (PMID:31848338). This antibody is CyTOF ready.
• Control:

  CXADR Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Adenovirus Antibody (8C4) - BSA Free

• Adenovirus

Background

Adenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). Forty-seven known serotypes have been isolated since they were first discovered in 1953 with 3 types known to cause gastroenteritis. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterized by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated causing disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Adenovirus Antibody (NB600-413)(9)

Publications using NB600-413

• He X, Yao W, Zhu JD et Al. Potent antitumor efficacy of human dental pulp stem cells armed with YSCH-01 oncolytic adenovirus J Transl Med 2023-10-05 [PMID: 37789452]
• Boilesen DR, Neckermann P, Willert T et al. Efficacy and synergy with cisplatin of an adenovirus vectored therapeutic E1E2E6E7 vaccine against HPV genome positive C3 cancers in mice Cancer immunology research 2022-12-19 [PMID: 36534088]
• Hassan AO, Kafai NM, Dmitriev IP et al. A Single-Dose Intranasal ChAd Vaccine Protects Upper and Lower Respiratory Tracts against SARS-CoV-2 Cell 2020-10-01 [PMID: 32931734]
• Fusciello M, Fontana F, TAhtinen S, et al. Artificially cloaked viral nanovaccine for cancer immunotherapy Nat Commun 2019-12-17 [PMID: 31848338] (B/N, Mouse)
• Kuryk L, Moller AW, Vuolanto A et al. Optimization of Early Steps in Oncolytic Adenovirus ONCOS-401 Production in T-175 and HYPERFlasks Int J Mol Sci 2019-01-31 [PMID: 30709038] (ICC/IF, Human)
• Ragonnaud E, Schroedel S, Mariya S et al. Replication deficient human adenovirus vector serotype 19a/64: Immunogenicity in mice and female cynomolgus macaques. Vaccine 2018-10-01 [PMID: 30190120] (Mouse, Cynomolgus Monkey)
• Garofalo, M, Saari, H Et al. Antitumor effect of oncolytic virus and paclitaxel encapsulated in extracellular vesicles for lung cancer treatment. J Control Release 2018-08-10 [PMID: 29864473] (WB)
• Garofalo M, Iovine B, Kuryk L et al. Oncolytic adenovirus loaded with L-carnosine as novel strategy to enhance the anti-tumor activity Mol. Cancer Ther. 2016-02-09 [PMID: 26861248] (ICC/IF, Virus)
• Fernes MS, Gomes EM, Butcher LD et al. Growth Inhibition of Human Multiple Myeloma Cells by an Oncolytic Adenovirus Carrying the CD40 Lig Transgene. Clin Cancer Res;15(15):4847-4856. 2009-01-01 [PMID: 19622582]

FAQs for Adenovirus Antibody (NB600-413). (Showing 1 - 1 of 1 FAQs).

1. What was the serotype of the immunogen used for the 8C4 antibody?
   • As an immunogen for NB600-413 we used both human adenovirus type 1 and canine adenovirus type 1 as an immunogen for this antibody.