Adiponectin/Acrp30 Antibody (19F1)

Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis of 8.4ug of rabbit subcutaneous white adipose tissue (scWAT), gonadal white adipose tissue (gWAT), perirenal white adipose tissue (pWAT), tibia marrow adipose tissue (tibMAT), tibia red marrow (tibRM), and 3T3-L1 positive control adipocyte lysates per well. Data courtesy of the Innovators Program.

Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Effect of whey peptides on 3T3-L1 adipocyte differentiation. 3T3-L1 preadipocytes were differentiated in the presence of 2.5 mg/mL WPI (whey protein isolate), WPH (whey peptide hydrolysate), BPI (BSA protein isolate) or BPH (BSA peptide hydrolysate). Immunoblotting and densitometric analysis of (A,D) adiponectin (n = 6). mRNA levels of (F) adiponectin (n = 8) were determined. * p < 0.05, **** p < 0.0001 vs. protein isolate controls. PS, protein stain. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32041341) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis of Adiponectin was performed using 10% confluent 3T3-L1 cells differentiated with Adipogenesis supplement for 5 days.

Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis was performed on biopsies of deparaffinized Human skin tissue.

Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis was performed using 10 ul of conditioned media of Hep G2 cell line (lane 1).

Immunohistochemistry: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Biopsies of deparaffinized Human skin tissue.

Immunocytochemistry/Immunofluorescence: Mouse Monoclonal Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Adipocytes isolated from the pelvic area of the male mouse. Image from a verified customer review.

• Reactivity: Hu, Mu, Rt, Rb
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 19F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Adiponectin/Acrp30 Antibody (19F1) Summary

• Immunogen: Full length, recombinant, human adiponectin.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADIPOQ
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 2 - 4 ug/ml
  • Immunohistochemistry 1:20 - 100
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1 ug/ml

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 30357746).Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Adiponectin/Acrp30 Antibody (19F1)

• ACDC
• Acrp30
• ACRP30ADPN
• adipocyte, C1Q and collagen domain containing
• Adiponectin
• adiponectin, C1Q and collagen domain containing
• AdipoQ
• ADIPQTL1
• ApM1
• apM-1
• APM1APM-1
• C1q and collagen domain-containing protein
• GBP28
• GBP28apM1
• Gelatin-binding protein

Background

Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Adiponectin/Acrp30 Antibody (NBP2-22450)(5)

Publications using NBP2-22450

• D'Souza K AUTOTAXIN IS NUTRITIONALLY REGULATED AND ALTERS MITOCHONDRIAL FUNCTION IN OBESITY-INDUCED INSULIN RESISTANCE Thesis (WB)
• D'Souza K, Mercer A, Mawhinney H et al. Whey Peptides Stimulate Differentiation and Lipid Metabolism in Adipocytes and Ameliorate Lipotoxicity-Induced Insulin Resistance in Muscle Cells Nutrients 2020-02-06 [PMID: 32041341] (WB, Mouse)
• Kang NY, Ivanovska J, Tamir-Hostovsky L et al. Chronic Intermittent Hypoxia in Premature Infants: The Link Between Low Fat Stores, Adiponectin Receptor Signaling and Lung Injury. Adv. Exp. Med. Biol. 2018-10-26 [PMID: 30357746] (WB, Rat)
• Sarmento-Cabral A, Luque RM. Adipokines and their receptor are widely expressed and distinctly regulated by the metabolic environment in the prostate of male mice: direct role under normal and tumoral conditions Endocrinology 2017-08-09 [PMID: 28938461] (Mouse)
• D'Souza K, Kane D A et al. Autotaxin Is Regulated by Glucose and Insulin in Adipocytes. Endocrinology 2017-01-04 [PMID: 28324037] (WB, Mouse)

Review for Adiponectin/Acrp30 Antibody (NBP2-22450) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Armen Petrosyan IF Mouse 11/06/2024

Summary

• Application: Immunofluorescence
• Sample Tested: Adipocytes
• Species: Mouse
• Lot: YJ381683

Bioinformatics

• Gene Symbol: ADIPOQ
• Entrez:
  • 9370(Human)
• Uniprot:
  • Q15848()