Adiponectin/Acrp30 Antibody

Western Blot: Adiponectin/Acrp30 Antibody [NB100-65810] - Western blot analysis of Adoponectin in 1. human liver 2. rat liver 3. HL60

Immunocytochemistry/Immunofluorescence: Adiponectin/Acrp30 Antibody [NB100-65810] - Adiponectin antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Cytoplasmic, membrane and internal organelle staining was observed.

Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody [NB100-65810] - Co-localization of C1q and APN deposited in adipose tissue and blood vessels (x100). Positive staining of C1q (red) and APN (green) is shown along the perivascular areas of fat tissues and intimal-medial layer of the blood vessel. C1q and APN co-localized almost completely in the same areas. H&E, hematoxylin and eosin. Image collected and cropped by CiteAb from the following publication (//www.cardiab.com/content/14/1/50), licensed under a CC-BY license.

Flow (Intracellular): Adiponectin/Acrp30 Antibody [NB100-65810] - An intracellular stain was performed on Jurkat cells with Adiponectin/Acrp30 Antibody NB100-65810AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to AF647.

Immunocytochemistry/Immunofluorescence: Adiponectin/Acrp30 Antibody [NB100-65810] - Immunocytochemical staining of HL60 cells with Rabbit anti Human adiponectin.

Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody [NB100-65810] - IHC analysis of Adiponectin in mouse liver showing nuclear and cytoplasmic staining.

Flow Cytometry: Adiponectin/Acrp30 Antibody [NB100-65810] - An intracellular stain was performed on Jurkat cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody.

Flow Cytometry: Adiponectin/Acrp30 Antibody [NB100-65810] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody.

Flow Cytometry: Adiponectin/Acrp30 Antibody [NB100-65810] - Analysis of Alexa Fluor 647 conjugate of NB100-65810. An intracellular stain was performed on Jurkat cells with Adiponectin antibody NB100-65810AF647 (blue) and a matched isotype control NBP2-24893AF647 (orange). Cells were fixed with 4% PFA and then perm

Flow Cytometry: Adiponectin/Acrp30 Antibody [NB100-65810] - Analysis of Allophycocyanin conjugate of NB100-65810. An intracellular stain was performed on Jurkat cells with Adiponectin antibody NB100-65810APC (blue) and a matched isotype control NBP2-24893APC (orange). Cells were fixed with 4% PFA and then permeabl

Flow Cytometry: Adiponectin/Acrp30 Antibody [NB100-65810] - Analysis of Allophycocyanin conjugate of NB100-65810. An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810APC (blue) and a matched isotype control NBP2-24893APC (orange). Cells were fixed with 4% PFA and then permeabliz

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Adiponectin/Acrp30 Antibody Summary

• Immunogen: A synthetic peptide made to a C-terminal sequence of human Adiponectin (between amino acids 200-244) [UniProt Q15848]
• Specificity: This recognises an epitope within the C-terminal region (CT) of the 244 amino acid protein Adiponectin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADIPOQ
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: The theoretical molecular weight of Adiponectin is 27, however this protein is highly glycosylated.
• Control:

  Adiponectin/Acrp30 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Adiponectin/Acrp30 Antibody

• ACDC
• Acrp30
• ACRP30ADPN
• adipocyte, C1Q and collagen domain containing
• Adiponectin
• adiponectin, C1Q and collagen domain containing
• AdipoQ
• ADIPQTL1
• ApM1
• apM-1
• APM1APM-1
• C1q and collagen domain-containing protein
• GBP28
• GBP28apM1
• Gelatin-binding protein

Background

Adiponectin, also known as 30 kDa adipocyte complement-related protein, adipocyte complement-related 30 kDa protein (ACRP30), adipocyte C1q and collagen domain-containing protein, adipose most abundant gene transcript 1 protein (apM-1), gelatin-binding protein, and ADIPOQ, is an important adipokine involved in the control of fat metabolism and insulin sensitivity. Unlike most other adipokines, Adiponectin is secreted exclusively by differentiating adipocytes at reduced levels during obesity. The amount of adiponectin in serum is inversely correlated with body mass index (BMI) of the individual, and has an anti-inflammatory action, playing an important role in type II diabetes (insulin sensitivity) and atherosclerosis. Defects to the adiponectin gene have been known to cause adiponectin deficiency and non-insulin dependent diabetes mellitus (NIDDM).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Adiponectin/Acrp30 Antibody (NB100-65810)(8)

Publications using NB100-65810

• Conti G, Calderan L, Quintero Sierra LA et al. Tumor and peritumoral adipose tissue crosstalk: de-differentiated adipocytes influence spread of colon carcinoma cells Tissue and Cell 2022-12-01 [PMID: 36542947] (WB, Mouse)
• Cho J, Teshigawara R, Kameda M et al. Nucleus-localized adiponectin is survival gate keeper through miR-214-mediated AIFM2 regulation Genes Cells 2018-11-25 [PMID: 30474186] (ICC/IF, Mouse)
• Waragai M, Adame A, Trinh I et al. Possible Involvement of Adiponectin, the Anti-Diabetes Molecule, in the Pathogenesis of Alzheimer's Disease. J. Alzheimers Dis. 2016-04-08 [PMID: 27079710] (IHC-P, Human)
• Hong ES, Lim C, Choi HY et al. The amount of C1q-adiponectin complex is higher in the serum and the complex localizes to perivascular areas of fat tissues and the intimal-medial layer of blood vessels of coronary artery disease patients. Cardiovasc Diabetol. 2015-05-14 [PMID: 25956582] (IHC-P, Human)
  
  Details:
  Adiponectin/Acrp30 antibody was used for IHC-P analysis of adipose/fat tissues (subcutaneous, visceral, and pericardial areas) and internal mammary artery sections from healthy controls and patients with coronary artery disease. The IHC-P assay implicated 4% paraformaldehyde fixation, ON 4C incubation of primary antibody and signal detection using Alexa Fluor 488 goat anti-rabbit IgG secondary antibody. Adiponectin/Acrp30 (APN) was found to be co-localized with complement component C1q along the perivascular areas of fat tissues and intimal–medial layer of the blood vessel (Figure 2).
• Canhoroz M, Kanat O, Saraydaroglu O et al. Clinical significance of adiponectin expression in colon cancer patients. J Cancer Res Ther 2014-07-15 [PMID: 25022390] (IHC-P, Human)
  
  Details:
  Adiponectin antibody used for IHC-P on human colon cancer patients. Primary diluted at 1:100 and incubated for 1 hour followed by detection with biolynated link reagent-DAB detection. Staining images not shown but see the full text for detailed IHC-P protocol.
• Trayhurn, P Wood, IS. Signalling role of adipose tissue: adipokines and inflammation in obesity. Biochem Soc Trans 33: 1078-1081. 2005-01-01 [PMID: 16246049]
• Lihn, AS et al. Adiponectin: action, regulation and association to insulin sensitivity. Obes Rev 6: 13-21. 2005-01-01 [PMID: 15655035]
• Wong, GW et al. A family of Acrp30/adiponectin structural functional paralogs. P.N.A.S. 101: 10302-10307. P.N.A.S. 101: 10302-10307. 2004-01-01 [PMID: 15231994] (IHC-P)

Bioinformatics

• Gene Symbol: ADIPOQ
• Entrez:
  • 9370(Human)
  • 246253(Rat)
• Uniprot:
  • Q15848()