ADP/ATP Ratio Assay Kit (Luminescent)

Product Discontinued

ADP/ATP Ratio Assay Kit (Luminescent) Summary

• Description: Bioluminescent detection of ADP and ATP levels for rapid screening of apoptosis, necrosis, growth arrest and cell proliferation simultaneously. Highly sensitive and high-throughput adaptable.
  
  Detection method: Luminometer or beta counterApplications: Bioluminescent detection of the ATP level via luciferase catalyzed reaction for a rapid screening of apoptosis and cell viability in mammalian cells.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65313)
• Specificity: The results can be analyzed using cuvette-based luminometers or Beta Counters. When Beta Counter is used it should be programmed in the out-of-coincidence (or Luminescence mode) for measurement.
• Kit Type: Assay Kit (Luminescent)

Packaging, Storage & Formulations

• Storage: Store at -20C.

Background

The changes in ADP/ATP ratio have been used to differentiate the different modes of cell death and viability. Increased levels of ATP and decreased levels of ADP have been recognized in proliferating cells. In contrast, decreased levels of ATP and increased levels of ADP are recognized in apoptotic cells. The decrease in ATP and increase in ADP are much more pronounced in necrosis than apoptosis. The ADP/ATP Ratio Assay kit utilizes bioluminescent detection of the ADP and ATP levels for a rapid screening of apoptosis, necrosis, growth arrest, and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter. ADP level is measured by its conversion to ATP that is subsequently detected using the same reaction. The assay can be fully automated for high throughput and is highly sensitive (detects 100 mammalian cells/well).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.