ADP Assay Kit (Colorimetric/Fluorometric)

Product Discontinued

ADP Assay Kit (Colorimetric/Fluorometric) Summary

• Description: ADP Colorimetric/Fluorometric Assay Kit: Robust and convenient colorimetric and fluorometric assay to measure ADP levels within an hour. Detection method: Fluorescent microscopy or FACS analysis (Ex/Em 485/530 nm) to detect stained live cells; (Ex/Em 495/635) to detect stained dead cells.
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab83359)
• Specificity: Stained live and dead cells can be visualized by fluorescence microscopy or FACS analysis.
• Kit Type: Assay Kit (Colorimetric/Fluorometric)

Packaging, Storage & Formulations

• Storage: Store at -20C.

Background

ADP is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. De-phosphorylation and rephosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. The newly designed ADP Assay Kit provides a convenient colorimetric and fluorometric means to measure ADP level. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (WLmax = 570 nm) or fluorometric method (Ex/Em 535/587 nm). The assay is simple, sensitive, stable and high-throughput adaptable. The assay can detect as low as 1 UM ADP in biological samples.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.