ADP-ribosylarginine hydrolase Antibody

Western Blot: ADP-ribosylarginine hydrolase Antibody [NBP2-97042] - Anti-ADP-ribosylarginine hydrolase rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 38 kDa (We are unsure as to the identity of these extra bands

Immunoprecipitation: ADP-ribosylarginine hydrolase Antibody [NBP2-97042] - ADP-ribosylarginine hydrolase was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate0.5 ul anti-ADP-ribosylarginine hydrolase rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ADP-ribosylarginine hydrolase rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 37 kDa

Product Discontinued

ADP-ribosylarginine hydrolase Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human ADP-ribosylarginine hydrolase (NP_001116.1; Met1-Leu351).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADPRH
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Affinity purified

Alternate Names for ADP-ribosylarginine hydrolase Antibody

• [Protein ADP-ribosylarginine] hydrolase
• ADP-ribosylarginine hydrolaseARH1ADP-ribose-L-arginine cleaving enzyme
• EC 3.2.2.19

Background

The enzyme encoded by this gene catalyzes removal of mono-ADP-ribose from arginine residues of proteins in the ADP-ribosylation cycle. Unlike the rat and mouse enzymes, which require DTT for maximal activity, the human enzyme is DTT-independent. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADPRH