AEC Chromogen/Substrate

Immunohistochemistry-Paraffin: AEC Chromogen/Substrate [NB900-79773] - Formalin fixed paraffin embedded human tonsil stained with high molecular weight Cytokeratin ab labelled with AEC Chromogen/Substrate (NB900-79773)

• Applications: IHC

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: Stable chromogen/substrate solution to be used in conjunction with peroxidase-based immunostaining systems. Specimens stained using AEC Chromogen/Substrate cannot be dehydrated in ethanol and hence need to be mounted in an aqueous based mounting medium.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Background

Even though DAB is much more sensitive chromogen compared to amino ethyl carbazol (AEC) because of its carcinogenic nature, some labs avoid using DAB. To resolve this problem, we have designed an AEC chromogen in liquid format. AEC is a chromogen of choice when performing immunoperoxidase staining. It has been well accepted amongst the pathologist because of its relatively less toxic nature compared to DAP. It produces a red colored end product at the positive sites which gives a good contrast with blue hematoxylin counter stain. Specimens stained in AEC cannot be dehydrated in ethanol and hence should be mounted in aqueous based mounting medium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.