AG-2/AGR2 Antibody [Unconjugated]

Western blot shows lysates of A549 human lung carcinoma cell line and human small intestine tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human AG-2/AGR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6068) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AG-2/AGR2 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

AG-2/AGR2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human AG-2/AGR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6068) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

AG-2/AGR2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human AG-2/AGR2
  Arg21-Leu175
  Accession # O95994
• Specificity: Detects human AG-2/AGR2 in direct ELISA and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant human AG-3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: AGR2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AG-2/AGR2 Antibody [Unconjugated]

• AG2
• AG-2
• AG2member 17
• AGR2
• anterior gradient 2 homolog (Xenopus laevis)
• anterior gradient homolog 2 (Xenopus laevis)
• GOB-4
• hAG-2
• HPC8
• PDIA17
• secreted cement gland homolog

Background

AG-2 (Anterior gradient protein 2; also AGR2, GOB4 and HPC8) is an 18‑21 kDa member of the PDI family of enzymes. It is expressed in secretory cell types, such as prostate epithelium, small intestine goblet, Paneth and enteroendocrine cells, and multiple carcinoma cell types. Although AG-2 possesses a signal sequence, it appears to be rarely secreted, instead being found in the ER due the presence of a KTEL ER retention signal at its C-terminus. AG-2 forms transient disulfide linkages with molecules destined for secretion, possibly aiding protein folding. Mature human AG-2 is 155 amino acids (aa) in length (aa 21‑175). Cys81 is presumed to participate in intermolecular bond formation. There is one potential alternative start site 20 aa upstream of the standard start site. Over aa 21‑175, human AG-2 shares 94% aa identity with mouse AG-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AG-2/AGR2 Antibody (AF6068)(3)

Publications using AF6068

• Fang, F;Liu, Y;Xiong, Y;Li, X;Li, G;Jiang, Y;Hou, X;Song, J; Slowed Intestinal Transit Induced by Less Mucus in Intestinal Goblet Cell Piezo1-Deficient Mice through Impaired Epithelial Homeostasis International journal of molecular sciences 2023-09-21 [PMID: 37762681] (IHC, Transgenic Mouse)
• Y Xu, Y Xiong, Y Liu, G Li, T Bai, G Zheng, X Hou, J Song Activation of goblet cell Piezo1 alleviates mucus barrier damage in mice exposed to WAS by inhibiting H3K9me3 modification Cell & bioscience, 2023-01-12;13(1):7. 2023-01-12 [PMID: 36631841] (IHC, Mouse)
• Ying Liu, Feifei Fang, Yilin Xiong, Jiandi Wu, Xueyan Li, Gangping Li et al. Reprogrammed fecal and mucosa-associated intestinal microbiota and weakened mucus layer in intestinal goblet cell- specific Piezo1-deficient mice Frontiers in Cellular and Infection Microbiology 2022-11-08 [PMID: 36425784]

Bioinformatics

• Gene Symbol: AGR2
• Uniprot:
  • O95994(Human)