AIF-1/Iba1 Antibody (1022-5)

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (1022-5) [NBP1-50156] - Rat brain tissue section stained with anti-Iba1 antibody. IHC-P image submitted by a verified customer review.

Product Discontinued

AIF-1/Iba1 Antibody (1022-5) Summary

• Immunogen: This AIF-1/Iba1 Antibody (1022-5) was made using a portion of the human IBA1 gene.
• Marker: pan-Microglia Marker
• Specificity: The monoclonal AIF-1/Iba1 Antibody (1022-5) is cross reactive with rat Allograft-inflammatory factor-1 (rat AIF-1). Human and rat amino acids sequences are 98% homologous.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: AIF1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunoassay 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:50
• Application Notes: This antibody works in Immunoassay, Immunohistochemistry-Paraffin, Western Blot. For immunohistology on paraffin sections, a combination of microwave and sodium citrate solution is recommended for antigen retrieval.
• Theoretical MW: 16.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27036089).

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS and 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for AIF-1/Iba1 Antibody (1022-5)

• AIF1
• AIF-1
• allograft inflammatory factor 1
• G1
• IBA1
• IbaI
• interferon gamma responsive transcript
• Ionized calcium-binding adapter molecule 1
• IRT1
• IRT-1
• Protein G1

Background

Allograft inflammatory factor (AIF-1) or ionized calcium-binding adapter molecule 1 (Iba1) is a cytosolic actin binding protein containing a calcium binding domain (EF-hand) and inducible by cytokines and IFN-gamma (1). AIF-1 (theoretical molecular weight 17kDa) was first cloned from rat and human cardiac allografts and macrophage cell lines. In cardiac allografts, expression of AIF-1 was specifically associated with infiltrated mononuclear cells (2, 3). AIF-1 is constitutively expressed and inducible in macrophages and microglia. Iba1, microglia response factor (MRF-1) and daintain were independently cloned and identified in rat and human tissues and share complete sequence identity with AIF-1. Additionally, several AIF-1 splice variants have been identified including IRT-1, BART-1, G1 and Hara-1 (2).

Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.

References

1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA. https://doi.org/10.1002/glia.10149

2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters. https://doi.org/10.1016/S0014-5793(02)02430-4

3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation. https://doi.org/10.1097/00007890-199605150-00018

4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells. https://doi.org/10.3390/cells8060639

5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T., ... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.5.3316

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AIF-1/Iba1 Antibody (NBP1-50156)(1)

Publication using NBP1-50156

• Kojo A, Yamada K, Yamamoto T. Glucose transporter 5 (GLUT5)-like immunoreactivity is localized in subsets of neurons and glia in the rat brain. J. Chem. Neuroanat. 2016-03-29 [PMID: 27036089] (IF/IHC, Rat)

Reviews for AIF-1/Iba1 Antibody (NBP1-50156) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Rat.

reviewed by: Verified Customer IHC-Fr Rat 08/09/2021

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Brain
• Species: Rat
• Lot: 1022-5

reviewed by: Victoria Andriulis IHC-Fr Mouse 08/09/2017

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: 4% PFA fixed frozen sections from mouse brain
• Species: Mouse
• Lot: 20442MO716-A

Bioinformatics

• Gene Symbol: AIF1
• Entrez:
  • 199(Human)