AIF-1/Iba1 Antibody

GM1 effects on brain microglia in vivo, after peripheral administration of LPS. Immunoblot in whole brain lysates and densitometric analysis show LPS-dependent increase in AIF-1/Iba1 expression. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34991625/) licensed under a CC-BY license.

GM1 effects on brain microglia in vivo, after peripheral administration of LPS. Immunoblot in whole brain lysates and densitometric analysis show LPS-dependent increase in AIF-1/Iba1 expression. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34991625/) licensed under a CC-BY license.

Reactive microglia (green) in peritumoral area of IC injected human glioblastoma cells in mouse brain. NeuN (red), DAPI (blue). Antibody at 1:250. IHC image submitted by a verified customer review.

AIF-1/Iba1 in canine brain. Primary antibody used at 1 ug/mL and incubated with tissue for 30 minutes at room temperature. IHC-P image submitted by a verified customer review.

Staining in mouse brain using AIF-1/Iba1 Antibody. IHC-P image submitted by a verified customer review.

Brown arrow= AIF-1/Iba1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and AIF-1/Iba1 positive cells. IHC-P image submitted by a verified customer review.

Staining in rat hippocampus using AIF-1/Iba1 Antibody. IHC-Fr image submitted by a verified customer review.

Feline optic nerve microglia were immunolabeled with AIF-1/Iba1 Antibody (green), DAPI (blue). Imaging was performed using an epifluorescence microscope. IHC-Fr image submitted by a verified customer review.

Mouse dorsal root ganglia. AIF-1/Iba1 expression in reactive macrophages. Maximum intensity z-projection from 3D images. IHC image submitted by a verified customer review.

Staining in mouse brain using AIF-1/Iba1 Antibody. IHC image submitted by a verified customer review.

Analysis of LPS injected mouse brain tissue, 20 um thickness, frozen. Primary antibody dilution, 1:200. Secondary antibody CY3. Mounted with DAPI mount. Image from verified customer review.

AIF-1/Iba1 staining of AD mouse model (hippocampus). Image from verified customer review.

Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

(0.1ug/ml) staining of Mouse Lymph node lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

(1ug/ml) staining of Human Frontal Cortex (A) and (0.1ug/ml) Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

(8ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.

Immunohistochemistry-Paraffin: Goat Polyclonal AIF-1/Iba1 Antibody [NB100-1028] - AIF-1/Iba1 immunoreactivity in an FFPE section of mouse spleen. NB100-1028 was diluted to 1ug per mL and was left on tissue sections for 30m at room temperature. Secondary was horse anti goat HRP polymer. Image from a verified customer review.

Immunohistochemistry-Paraffin: Goat Polyclonal AIF-1/Iba1 Antibody [NB100-1028] - AIF-1/Iba1 immunoreactivity in an FFPE section of human tonsil. NB100-1028 was diluted to 1ug per mL and was left on tissue sections for 30m at room temperature. Secondary was horse anti goat HRP polymer. Image from a verified customer review.

Immunohistochemistry-Paraffin: Goat Polyclonal AIF-1/Iba1 Antibody [NB100-1028] - AIF-1/Iba1 immunoreactivity in an FFPE section of equine spleen. NB100-1028 was diluted to 1ug per mL and was left on tissue sections for 30m at room temperature. Secondary was equine anti goat HRP polymer. Image from a verified customer review.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - Microglia proliferate in non‐APP mice upon peripheral immune challengeA, BIba‐1, Ki67, & DAPI staining in the cortex & hippocampus of wild‐type & Nlrp3−/− (5 & 15 months old). Microglia proliferate upon LPS injection (white arrows). Non‐microglia cells proliferation was observed as well (yellow arrows). Scale bar: 20 μm.CQuantification of microglial proliferation in cortex (left panel) & hippocampus (right panel) (mean of 5 ± SEM; two‐way ANOVA followed by Tukey's post hoc test, *P 1.5, FDR-corrected p1.5, FDR-corrected p<0.05) to identify GO terms, including inflammatory response (b). The volcano plot shows significant DEGs derived from the NanoString analysis of the brains of Plcg2 inactivation (PLCG2 inact, n=3) & WT (n=3) mice (1-month male) (c). Immunostaining of microglial markers with IBA1 (green) & P2RY12 (red) & quantification of percent area in brain sections of 1-month-old male WT & PLCG2 inact demonstrate unchanged microglial morphology & area coverage (d). GO gene ontology, BP biological process, DEGs differentially expressed genes, FDR false discovery rate Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35180881), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - Representative & quantitative immunohistochemistry of the peri-infarct cortex before the bregma (coordinate + 0.58) in normal WT, normal sEH KO, MCAO WT, MCAO sEH KO, MCAO with vehicle treatment & MCAO with AUDA treatment mice (n = 2 for normal groups, n = 5–6 for all groups subjected to MCAO). (A–F, S) The number of CD31-positive endothelial cells significantly increased in the sEH KO before & after MCAO & post-MCAO AUDA-treated mice, while (G–L) Note the increased phosphorylated TrkB (p-TrkB) immunoreactivity that partially co-localized with NeuN-positive neurons, particularly in the sEH KO & AUDA-treated mice (arrows) (T). The percentage of NeuN- & p-TrkB-positive double labeling in the neuronal population was significantly increased compared to that in the corresponding controls. (M–R, U–V) GFAP-positive astrogliosis & Iba1-positive microglial infiltration decreased in post-MCAO sEH KO & AUDA treatment groups relative to that in the corresponding controls. The scale bar represents 50 µm. *p < 0.05, **p < 0.01, ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29311641), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - Representative & quantitative immunohistochemistry of the peri-infarct cortex before the bregma (coordinate + 0.58) in normal WT, normal sEH KO, MCAO WT, MCAO sEH KO, MCAO with vehicle treatment & MCAO with AUDA treatment mice (n = 2 for normal groups, n = 5–6 for all groups subjected to MCAO). (A–F, S) The number of CD31-positive endothelial cells significantly increased in the sEH KO before & after MCAO & post-MCAO AUDA-treated mice, while (G–L) Note the increased phosphorylated TrkB (p-TrkB) immunoreactivity that partially co-localized with NeuN-positive neurons, particularly in the sEH KO & AUDA-treated mice (arrows) (T). The percentage of NeuN- & p-TrkB-positive double labeling in the neuronal population was significantly increased compared to that in the corresponding controls. (M–R, U–V) GFAP-positive astrogliosis & Iba1-positive microglial infiltration decreased in post-MCAO sEH KO & AUDA treatment groups relative to that in the corresponding controls. The scale bar represents 50 µm. *p < 0.05, **p < 0.01, ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29311641), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - Norgestrel reduces pro-inflammatory & promotes anti-inflammatory phenotypes in rd10 microglia.(A) RT-qPCR analysis detected similar levels of mRNAs encoding progesterone receptor membrane complexes 1 & 2 (PGRMC1, PGRMC2) & all three membrane progesterone receptor isoforms, alpha , beta & gamma (mPR alpha , mPR beta , mPR gamma ) in rd10 retinal microglial cells. Classical progesterone receptor (PR A/B) was not expressed. Actin, HPRT & GAPDH served as reference genes. (B) Immunolabeling confirmed the presence of these progesterone receptors in primary rd10 microglial cells. Hoechst (blue) staining reveals the cell nuclei. Scale bar represents 10μm. (C) Fluorescent microscopic images of primary rd10 microglia immunolabeled for (C) (i) markers of activation (CD68) & pro-inflammation (M1) (iNOS) & (C) (ii) anti-inflammatory activation (M2) (MRC1); after treatment with 20 μM Norgestrel over 24h. Norgestrel reduced pro-inflammatory & increased anti-inflammatory markers in rd10 microglia. Scale bar 10μm. (D) Quantification of fluorescence intensity of (D) (i) CD68 & iNOS & (D) (ii) MRC1 in primary rd10 microglial cultures following Norgestrel treatment over 24h. N = 8 mice, n = 4 technical replicates per group. (E) Nitrite concentration in the media of rd10 microglia in vitro treated with Norgestrel or vehicle over 24hr. Norgestrel significantly reduced nitrite release from rd10 microglia. N = 8 biological replicates, n = 2 technical replicates per sample. Results are presented as mean ± SEM (t-test, *p<0.05, ***p<0.005, ****p<0.001). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0165197), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: AIF-1/Iba1 Antibody [NB100-1028] - Temporal activation of caspase-8 in Iba1-positive myeloid cells. Frozen tissue sections from mice upon pMCAO (n = 9) were analyzed by immunohistochemistry for expression of active caspase-8 or for expression of the microglia/macrophage marker Iba1. The contralateral side to the ischemic area was used an internal control for each animal. A small increase in body size of Iba1 positive cells was detected in the peri-infarct area at 6 h, with larger increase in cell body & numbers of cells expressing Iba1 detected at 24 & 48 h post occlusion (a). Cleaved caspase-8 was detected at low levels at 6 h, but a significant increase in expression levels was observed at 24 & 48 h in the peri-infarct area, but not at the contralateral side (a). Double immunofluorescent staining revealed that increased active caspase-8 expression was localized in Iba1-positive cells (b). Scale bar represent 100 μm in IHC images (a) & 10 μm in IF images (b) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27566702), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: AIF-1/Iba1 Antibody [NB100-1028] - GM1 effects on brain microglia in vivo, after peripheral administration of LPS. A Schematic representation of in vivo administration of GM1 after LPS-induced systemic inflammation in mice (N = 5 per treatment). B Representative images of Iba-1 stained microglia in the cortex of control animals infused with vehicle (CTRL) or GM1 (CTRL + GM1), & in animals treated with LPS & subsequently infused with vehicle artificial cerebrospinal fluid (LPS) or GM1 (LPS + GM1). C Immunoblot in whole brain lysates & densitometric analysis show LPS-dependent increase in Iba 1 expression. D Cortical & striatal microglia (Iba-1+ cells) cell body area quantified with MetaXpress software. GM1 administration significantly decreases cell body area in the cortex, but not in the striatum. E IL-6 protein levels in whole mouse brain homogenate. aCSF, artificial cerebrospinal fluid. Bars show mean values ± STDEV. Two-way ANOVA with Tukey’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34991625), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - ABIN1 knockdown increases proinflammatory cytokine production & microglial activation. (a-b) Levels of the ABIN1 mRNA & protein were detected 24 h after reperfusion using RT-qPCR & western blot, respectively, to confirm the efficiency of ABIN1 gene knockdown (n = 5 rats per group). (c) ELISA was used to detect the concentrations of TNF-alpha , IL-1 beta & MCP-1 in the peri-infarct cortex at 24 h after reperfusion (n = 5 rats per group). (d) Microglial morphology was observed using immunofluorescence staining for Iba-1 (green) in the peri-infarct cortex at 24 h after reperfusion (n = 3 rats per group). Scale bar = 50 μm. (e & f) Quantification of microglia process endpoints & cell & process lengths/cell (∗∗P < 0.01 compared to the sham group; ##P < 0.01 compared to the MCAO/R group). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33110436), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - SCF+G-CSF treatment increases TREM2 expression in the Iba1+ microglia/macrophages surrounding the 6E10+ senile plaques. (A) Representative confocal images of TREM2 (red), 6E10 (purple) & Iba1 (green) triple immunofluorescence staining in the brains of aged APP/PS1 mice. Blue: Nuclear counterstaining by DAPI. (B) Representative orthographic view of z-stack images (12 z-stacks with 1μm intervals) illustrates the location & interaction of TREM2 + cells (red) & 6E10+ A beta plaques (white) in the brains of aged APP/PS1 mice. (C) Quantification data show the percentage of TREM2+ area surrounding the 6E10+ A beta plaques (within 10μm from the border of the A beta plaques) in the brains of aged APP/PS1 mice with or without SCF+G-CSF treatment. (D) Representative orthographic view of z-stack images (12 z-stacks with 1μm intervals) displays the location & interaction of TREM2+/Iba1+ co-expressing cells (yellow) & 6E10+ A beta plaques (white) in the brains of APP/PS1 mice. (E) Quantification data show the percentage of TREM2+/Iba1+ co-expression area in the total of Iba1+ area in the vicinity of 6E10+ A beta plaques in the brains of aged APP/PS1 mice with or without SCF+G-CSF treatment. N=4-5. Mean ± SEM. * p<0.05 by Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33269098), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - SCF+G-CSF treatment increases the association of Iba1+ microglia/macrophages with senile plaques & enhances uptake of 4G8+ A beta by the Iba1+ microglia/macrophages in the brains of aged APP/PS1 mice. (A) Representative confocal images illustrate the association of Iba1+ cells (green) with 4G8+ A beta plaques (red) in the brains of aged APP/PS1 mice. Blue: Nuclear counterstaining by DAPI. (B) Quantification of the percentage of Iba1+ area in/surrounding the 4G8+ A beta plaques in the brains of aged APP/PS1 mice with or without SCF+G-CSF treatment. (C) Representative 3-dimensional projected images reveal the overlapped (yellow) Iba1+ cells (green) with 4G8+ A beta (red) in the brains of aged APP/PS1 mice. The white arrows indicate the co-expression (yellow) of 4G8+ A beta & Iba1+ cells in the brains of aged APP/PS1 mice. (D & E) Representative orthographic view of z-stack images (12 z-stacks with 1μm intervals) shows the co-expression (yellow) of 4G8+ A beta (red) & Iba1+ cells (green) in the brains of aged APP/PS1 mice. (F) Quantification data present the percentage of 4G8+ A beta volume within the Iba1+ cells in the brains of aged APP/PS1 mice treated with or without SCF+G-CSF. N=4-5. Mean ± SEM. *p<0.05 by Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33269098), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - Representative & quantitative immunohistochemistry of the peri-infarct cortex before the bregma (coordinate + 0.58) in normal WT, normal sEH KO, MCAO WT, MCAO sEH KO, MCAO with vehicle treatment & MCAO with AUDA treatment mice (n = 2 for normal groups, n = 5–6 for all groups subjected to MCAO). (A–F, S) The number of CD31-positive endothelial cells significantly increased in the sEH KO before & after MCAO & post-MCAO AUDA-treated mice, while (G–L) Note the increased phosphorylated TrkB (p-TrkB) immunoreactivity that partially co-localized with NeuN-positive neurons, particularly in the sEH KO & AUDA-treated mice (arrows) (T). The percentage of NeuN- & p-TrkB-positive double labeling in the neuronal population was significantly increased compared to that in the corresponding controls. (M–R, U–V) GFAP-positive astrogliosis & Iba1-positive microglial infiltration decreased in post-MCAO sEH KO & AUDA treatment groups relative to that in the corresponding controls. The scale bar represents 50 µm. *p < 0.05, **p < 0.01, ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29311641), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - Antibody-mediated Nogo-A blockade decreases the number of cells expressing TNF alpha without affecting the number of Iba1-positive monocytes.Cells expressing TNF alpha , Iba1, & CD68 were labeled on cryosections by immunofluorescence in 4 mouse retinae, 24 h after control IgG or 11C7 injection. a Retinal sections showed many TNF alpha -containing cells located in the inner retina, between the outer plexiform layer & the ganglion cell layer 1 day after control IgG injection. A majority of cells was positive for Iba1 & exhibited vesicular staining of the lysosomal protein CD68, indicating their active state. b The injection of 11C7 strongly attenuated TNF alpha & CD68 expression & to a lower extent the intensity of Iba1 in macrophages/microglia. c Quantification of the number of cells expressing TNF alpha & Iba1 suggesting potent & specific effects of 11C7 on TNF alpha cytokine reduction. Six sections were examined for each retina. Statistics: one-way ANOVA, Dunnett’s post hoc test, **P < 0.01; ****P < 0.0001. Scale bars = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32029703), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - SCF+G-CSF treatment increases TREM2 expression in the Iba1+ microglia/macrophages surrounding the 6E10+ senile plaques. (A) Representative confocal images of TREM2 (red), 6E10 (purple) & Iba1 (green) triple immunofluorescence staining in the brains of aged APP/PS1 mice. Blue: Nuclear counterstaining by DAPI. (B) Representative orthographic view of z-stack images (12 z-stacks with 1μm intervals) illustrates the location & interaction of TREM2 + cells (red) & 6E10+ A beta plaques (white) in the brains of aged APP/PS1 mice. (C) Quantification data show the percentage of TREM2+ area surrounding the 6E10+ A beta plaques (within 10μm from the border of the A beta plaques) in the brains of aged APP/PS1 mice with or without SCF+G-CSF treatment. (D) Representative orthographic view of z-stack images (12 z-stacks with 1μm intervals) displays the location & interaction of TREM2+/Iba1+ co-expressing cells (yellow) & 6E10+ A beta plaques (white) in the brains of APP/PS1 mice. (E) Quantification data show the percentage of TREM2+/Iba1+ co-expression area in the total of Iba1+ area in the vicinity of 6E10+ A beta plaques in the brains of aged APP/PS1 mice with or without SCF+G-CSF treatment. N=4-5. Mean ± SEM. * p<0.05 by Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33269098), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - Representative & quantitative immunohistochemistry of the peri-infarct cortex before the bregma (coordinate + 0.58) in normal WT, normal sEH KO, MCAO WT, MCAO sEH KO, MCAO with vehicle treatment & MCAO with AUDA treatment mice (n = 2 for normal groups, n = 5–6 for all groups subjected to MCAO). (A–F, S) The number of CD31-positive endothelial cells significantly increased in the sEH KO before & after MCAO & post-MCAO AUDA-treated mice, while (G–L) Note the increased phosphorylated TrkB (p-TrkB) immunoreactivity that partially co-localized with NeuN-positive neurons, particularly in the sEH KO & AUDA-treated mice (arrows) (T). The percentage of NeuN- & p-TrkB-positive double labeling in the neuronal population was significantly increased compared to that in the corresponding controls. (M–R, U–V) GFAP-positive astrogliosis & Iba1-positive microglial infiltration decreased in post-MCAO sEH KO & AUDA treatment groups relative to that in the corresponding controls. The scale bar represents 50 µm. *p < 0.05, **p < 0.01, ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29311641), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - Representative & quantitative immunohistochemistry of the peri-infarct cortex before the bregma (coordinate + 0.58) in normal WT, normal sEH KO, MCAO WT, MCAO sEH KO, MCAO with vehicle treatment & MCAO with AUDA treatment mice (n = 2 for normal groups, n = 5–6 for all groups subjected to MCAO). (A–F, S) The number of CD31-positive endothelial cells significantly increased in the sEH KO before & after MCAO & post-MCAO AUDA-treated mice, while (G–L) Note the increased phosphorylated TrkB (p-TrkB) immunoreactivity that partially co-localized with NeuN-positive neurons, particularly in the sEH KO & AUDA-treated mice (arrows) (T). The percentage of NeuN- & p-TrkB-positive double labeling in the neuronal population was significantly increased compared to that in the corresponding controls. (M–R, U–V) GFAP-positive astrogliosis & Iba1-positive microglial infiltration decreased in post-MCAO sEH KO & AUDA treatment groups relative to that in the corresponding controls. The scale bar represents 50 µm. *p < 0.05, **p < 0.01, ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29311641), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - The presence or absence of TRPV1 influences microglial pathophysiological changes in recurrent febrile seizures mice. (A) Images of the cortex immunostained for the microglial marker Iba-1 (visualized in green FITC) & the lysosomal marker CD68/ED1 (visualized in red Alexa Fluor 594) & DAPI (visualized in blue) 5 days after rFS from wild type (WT) & TRPV1−/− mice (Scale bars: 50 μm). (B,C) Bar graph of quantification of Iba1+(B) & Iba1+ ED1+(C) in 5 days after rFS mice cortex. (D) Representative photomicrographs of different microglial morphological subtypes including resting/ramified (Ram), hypertrophic/intermediate (Inter) & round/ameboid (Amoeboid; Scale bars: 5 μm). (E) Quantification of relative percentage of microglia with different morphologies in the cortex of WT & TRPV1−/− mice (n = 6 per groups, **p < 0.01, ***p < 0.001, two-way ANOVA followed by Tukey’s multiple comparisons). (F–H) Representative Immunoblot bands (F) & densitometric analysis of Iba1 expression (G) & ED1 expression (H) were significantly increased in rFS from WT mice than in TRPV1−/− mice. Data were presented as means ± SEM, n = 6 per groups, *p < 0.05, **p < 0.01, ***p < 0.001, two-way ANOVA followed by Tukey’s multiple comparisons. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31680864), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Frozen: Goat Polyclonal AIF-1/Iba1 Antibody [NB100-1028] - AD Mouse Model. FxFr:10 µm thickness 1:200. Secondary antibody AF647. Image from a verified customer review.

Immunohistochemistry: Goat Polyclonal AIF-1/Iba1 Antibody [NB100-1028] - Pax5 and Iba1 immunoreactivity in a formalin fixed paraffin embedded classic Hodgkin's lymphoma biopsy. Formalin fixed paraffin embedded sections were deparaffinized and rehydrated. Sections then underwent heat induced epitope retrieval in a citrate-based solution for 20min in a food steamer. Sections were cooled, then blocked in phosphate buffered saline containing 0.2% Triton-X100 and 10% normal donkey serum for 1h at room temperature. Sections were then incubated with the primary antibody for 16h at room temperature. The following day slides were washed three times with PBS containing 0.05% Tween-20. Sections were then incubated with donkey anti-goat (647 conjugate) at 5ug per mL for 45min at room temperature. Slides were then washed as above and mounted with a coverslip. Image from a verified customer review.

• Reactivity: Hu, Mu, Rt, Ca, Eq, Fe, Po
• Applications: WB, ELISA, ICC/IF, IHC, Dual ISH-IHC, IHC, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

AIF-1/Iba1 Antibody Summary

• Immunogen: This AIF-1/Iba1 Antibody was developed against a peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.
• Marker: pan-Microglia Marker
• Specificity: This AIF-1/Iba1 Antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3). AIF-1/Iba1 is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.
• Predicted Species: Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AIF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dual RNAscope ISH-IHC
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 6 - 8 ug/mL
  • Knockout Validated
  • Peptide ELISA Detection Limit 1:128000
  • Western Blot 0.1 - 1.0 ug/mL
• Application Notes: ICC/IF use reported in scientific literature (PMID: 26335282). Use in IHC-Whole mount reported in scientific literature (PMID: 26676316). This AIF-1/Iba1 antibody is validated for IHC-Whole mount and IHC-F from verified customer reviews. Dual ISH-IHC validation reported in scientific literature (PMID: 31220182). WB: Approx. 16kDa band observed in Human Frontal Cortex, Mouse Brain and Rat Brain lysates and Mouse Lymph node lysates (calculated MW of 16.7kDa according to Human NP_001614.3, 16.9kDa according to Mouse NP_062340.1 and 16.8kDa according to Rat NP_058892.1). Primary incubation 1 hour at room temperature.
• Theoretical MW: 16.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  AIF-1/Iba1 Overexpression Lysate

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Reactivity Notes

Use in Rat reported in scientific literature (PMID: 33704579). Feline, canine and equine reactivity reported from verified customer reviews.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AIF-1/Iba1 Antibody

• AIF1
• AIF-1
• allograft inflammatory factor 1
• G1
• IBA1
• IbaI
• interferon gamma responsive transcript
• Ionized calcium-binding adapter molecule 1
• IRT1
• IRT-1
• Protein G1

Background

Allograft inflammatory factor (AIF-1) or ionized calcium-binding adapter molecule 1 (Iba1) is a cytosolic actin binding protein containing a calcium binding domain (EF-hand) and inducible by cytokines and IFN-gamma (1). AIF-1 (theoretical molecular weight 17kDa) was first cloned from rat and human cardiac allografts and macrophage cell lines. In cardiac allografts, expression of AIF-1 was specifically associated with infiltrated mononuclear cells (2, 3). AIF-1 is constitutively expressed and inducible in macrophages and microglia. Iba1, microglia response factor (MRF-1) and daintain were independently cloned and identified in rat and human tissues and share complete sequence identity with AIF-1. Additionally, several AIF-1 splice variants have been identified including IRT-1, BART-1, G1 and Hara-1 (2).

Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.

References

1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA. https://doi.org/10.1002/glia.10149

2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters. https://doi.org/10.1016/S0014-5793(02)02430-4

3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation. https://doi.org/10.1097/00007890-199605150-00018

4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells. https://doi.org/10.3390/cells8060639

5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T., ... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.5.3316

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AIF-1/Iba1 Antibody (NB100-1028)(491)

Publications using NB100-1028

• Lisi L, Olivi A, Ciotti GMP et Al. A topographic approach to the markers of macrophage/microglia and other cell types in high grade glioma Neurochem Int 2024-12-27 [PMID: 39734023]
• Horiuchi, M;Watanabe, S;Komine, O;Takahashi, E;Kaneko, K;Itohara, S;Shimada, M;Ogi, T;Yamanaka, K; ALS-linked mutant TDP-43 in oligodendrocytes induces oligodendrocyte damage and exacerbates motor dysfunction in mice Acta neuropathologica communications 2024-11-27 [PMID: 39605053]
• Sobue A, Komine O, Endo F et Al. Microglial cannabinoid receptor type II stimulation improves cognitive impairment and neuroinflammation in Alzheimer's disease mice by controlling astrocyte activation Cell Death Dis 2024-11-26 [PMID: 39587077]
• Nagarajan G, Zhang Y., et Al. Distinct expression profile reveals glia involvement in the trigeminal system attributing to post-traumatic headache J Headache Pain 2024-11-22 [PMID: 39578726]
• Mey GM, Evonuk KS, Shelestak J et Al. Inhibiting AMPA receptor signaling in oligodendrocytes rescues synapse loss in a model of autoimmune demyelination iScience 2024-10-22 [PMID: 39569383]
• Root J, Mendsaikhan A, Taylor G, Merino P et Al. Granulins rescue inflammation, lysosome dysfunction, lipofuscin, and neuropathology in a mouse model of progranulin deficiency Cell Rep 2024-11-20 [PMID: 39565694]
• Guo JL, Braun D, Fitzgerald GA et Al. Decreased lipidated ApoE-receptor interactions confer protection against pathogenicity of ApoE and its lipid cargoes in lysosomes Cell 2024-11-11 [PMID: 39532095]
• Andrew PM, MacMahon JA, Bernardino PN, Tsai YH et Al. Shifts in the spatiotemporal profile of inflammatory phenotypes of innate immune cells in the rat brain following acute intoxication with the organophosphate diisopropylfluorophosphate J Neuroinflammation 2024-11-04 [PMID: 39497181]
• Franco AC, Martini H, Victorelli S et al. Senescent cell transplantation into the skin induces age-related peripheral dysfunction and cognitive decline. Aging cell 2024-10-07 [PMID: 39374134]
• Makarava N, Safadi T, Bocharova O et Al. Reactive microglia partially envelop viable neurons in prion diseases J Clin Invest 2024-10-03 [PMID: 39361421]
• Torres M, Pederson B, Wang H et Al. Purkinje cell-specific deficiency in SEL1L-hrd1 endoplasmic reticulum-associated degradation causes progressive cerebellar ataxia in mice JCI Insight 2024-11-08 [PMID: 39352758]
• Chen, J;Crouch, EE;Zawadzki, ME;Jacobs, KA;Mayo, LN;Choi, JJ;Lin, PY;Shaikh, S;Tsui, J;Gonzalez-Granero, S;Waller, S;Kelekar, A;Kang, G;Valenzuela, EJ;Birrueta, JO;Diafos, LN;Wedderburn-Pugh, K;Di Marco, B;Xia, W;Han, CZ;Coufal, NG;Glass, CK;Fancy, SPJ;Alfonso, J;Kriegstein, AR;Oldham, MC;Garcia-Verdugo, JM;Kutys, ML;Lehtinen, MK;Combes, AJ;Huang, EJ; Proinflammatory immune cells disrupt angiogenesis and promote germinal matrix hemorrhage in prenatal human brain Nature neuroscience 2024-09-30 [PMID: 39349662]
• Kim MJ, Lee D, Ryu JH et Al. Weisheng-tang protects against ischemic brain injury by modulating microglia activation through the P2Y12 receptor Front Pharmacol 2024-09-04 [PMID: 39295932]
• Pei J, Xiong L, Wang X et Al. Dynamic changes in cellular atlases and communication patterns within yak ovaries across diverse reproductive states unveiled by single-cell analysis Front Cell Dev Biol 2024-08-29 [PMID: 39268087]
• Profaci CP, Harvey SS, Bajc K et Al. Microglia are not necessary for maintenance of blood-brain barrier properties in health, but PLX5622 alters brain endothelial cholesterol metabolism Neuron 2024-08-08 [PMID: 39142282]
• Kloosterman DJ, Erbani J, Boon M et Al. Macrophage-mediated myelin recycling fuels brain cancer malignancy Cell 2024-09-19 [PMID: 39137777]
• Scheiblich H, Eikens F, Wischhof L, Opitz S et Al. Microglia rescue neurons from aggregate-induced neuronal dysfunction and death through tunneling nanotubes Neuron 2024-07-26 [PMID: 39059388]
• Kenta Makabe, Hiroyuki Okada, Naohiro Tachibana, Hisatoshi Ishikura, Norihito Ito, Masaru Tanaka, Ryota Chijimatsu, Asuka Terashima, Fumiko Yano, Meiko Asaka, Dai Yanagihara, Shuji Taketomi, Takumi Matsumoto, Sakae Tanaka, Yasunori Omata, Taku Saito Baricitinib ameliorates inflammatory and neuropathic pain in collagen antibody-induced arthritis mice by modulating the IL-6/JAK/STAT3 pathway and CSF-1 expression in dorsal root ganglion neurons Arthritis Research & Therapy 2024-06-15 [PMID: 38879555]
• Wang N, Cai L, Pei X, Lin Z et Al. Microglial apolipoprotein E particles contribute to neuronal senescence and synaptotoxicity iScience 2024-06-13 [PMID: 38868202]
• Seokjo Kang, Emily Y. Ko, Amelia E. Andrews, Juliana E. Shin, Karina J. Nance, Pijus K. Barman, Peter S. Heeger, Willard M. Freeman, Bérénice A. Benayoun, Helen S. Goodridge Microglia undergo sex-dimorphic transcriptional and metabolic rewiring during aging Journal of Neuroinflammation 2024-06-05 [PMID: 38840206]
• Zhang F, Pan L, Lian C et Al. ICAM-1 may promote the loss of dopaminergic neurons by regulating inflammation in MPTP-induced Parkinson's disease mouse models Brain Res Bull 2024-06-16 [PMID: 38825252]
• Huang, Y;Wang, M;Ni, H;Zhang, J;Li, A;Hu, B;Junqueira Alves, C;Wahane, S;Rios de Anda, M;Ho, L;Li, Y;Kang, S;Neff, R;Kostic, A;Buxbaum, JD;Crary, JF;Brennand, KJ;Zhang, B;Zou, H;Friedel, RH; Regulation of cell distancing in peri-plaque glial nets by Plexin-B1 affects glial activation and amyloid compaction in Alzheimer's disease Nature neuroscience 2024-05-27 [PMID: 38802590]
• Guan X, Zhu S, Song J et Al. Microglial CMPK2 promotes neuroinflammation and brain injury after ischemic stroke Cell Rep Med 2024-05-03 [PMID: 38701781]
• Chandía-Cristi A, Gutiérrez DA, Dulcey AE et Al. Prophylactic treatment with the c-Abl inhibitor, neurotinib, diminishes neuronal damage and the convulsive state in pilocarpine-induced mice Cell Rep 2024-04-23 [PMID: 38656874]
• Chandrama Ahmed, Hendrik J. Greve, Carla Garza‐Lombo, Jamie A. Malley, James A. Johnson, Adrian L. Oblak, Michelle L. Block Peripheral HMGB1 is linked to O 3 pathology of disease‐associated astrocytes and amyloid Alzheimer's & Dementia 2024-04-16 [PMID: 38624088]
• Laurent Calvier, Anna Alexander, Austin T. Marckx, Maria Z. Kounnas, Murat Durakoglugil, Joachim Herz, Bernhard Ryffel Safety of Anti-Reelin Therapeutic Approaches for Chronic Inflammatory Diseases Cells 2024-03-27 [PMID: 38607022]
• Schrader, JM;Xu, F;Agostinucci, KJ;DaSilva, NA;Van Nostrand, WE; Longitudinal markers of cerebral amyloid angiopathy and related inflammation in rTg-DI rats Scientific reports 2024-04-10 [PMID: 38600214]
• Holden JM, Wareham LK, Calkins DJ. et Al. Morphological and electrophysiological characterization of a novel displaced astrocyte in the mouse retina Glia 2024-05-09 [PMID: 38591270]
• Steven F. Abcouwer, Bruna Miglioranza Scavuzzi, Phillip E. Kish, Dejuan Kong, Sumathi Shanmugam, Xuan An Le, Jingyu Yao, Heather Hager, David N Zacks The mouse retinal pigment epithelium mounts an innate immune defense response following retinal detachment Journal of Neuroinflammation 2024-03-25 [PMID: 38528525]
• Feng T, Du H, Yang C et Al. Loss of TMEM106B exacerbates Tau pathology and neurodegeneration in PS19 mice Acta Neuropathol 2024-03-27 [PMID: 38526799]
• Bhusal, A;Kim, JH;Kim, SC;Hwang, EM;Ryu, H;Ali, MS;Park, SC;Lee, WH;Suk, K; The microglial innate immune protein PGLYRP1 mediates neuroinflammation and consequent behavioral changes Cell reports 2024-02-22 [PMID: 38393947]
• Ting Wang, Akira Sobue, Seiji Watanabe, Okiru Komine, Takaomi C Saido, Takashi Saito, Koji Yamanaka Dimethyl fumarate improves cognitive impairment and neuroinflammation in mice with Alzheimer's disease. Journal of neuroinflammation 2024-02-23 [PMID: 38383481]
• Kathleen Margaret Hagen, Paul Gordon, Ariana Frederick, Alexandra Louise Palmer, Pariya Edalat, Yohan Ricci Zonta, Lucas Scott, Melissa Flancia, Jacqueline Kelsey Reid, Matthew Joel, Shalina Sheryl Ousman CRYAB plays a role in terminating the presence of pro-inflammatory macrophages in the older, injured mouse peripheral nervous system. Neurobiology of aging 2024-02-23 [PMID: 38381471]
• Zengyong Huang, Zhuo Gong, Yongtai Lin, Fan Yang, Weiping Chen, Shaotong Xiang, Yuedong Huang, Hao Xiao, Shuwen Xu, Jinhai Duan Treatment with glatiramer acetate in APPswe/PS1dE9 mice at an early stage of Alzheimer’s disease prior to amyloid-beta deposition delays the disease’s pathological development and ameliorates cognitive decline Frontiers in Aging Neuroscience 2024-01-30 [PMID: 38352237]
• Komine O, Ohnuma S, Hinohara K, Hara Y et Al. Genetic background variation impacts microglial heterogeneity and disease progression in amyotrophic lateral sclerosis model mice iScience 2024-02-06 [PMID: 38318390]
• Wan-Rong Jiang, Yong-Ming Zhou, Wei Wu, Li-Jie Yang, You Wu, Xin-Yuan Zhang, Zhao-Hui Yao A circRNA ceRNA network involved in cognitive dysfunction after chronic cerebral hypoperfusion Aging (Albany NY) 2024-01-31 [PMID: 38231472]
• Simkin J, Aloysius A, Adam M, Safaee F et Al. Tissue-resident macrophages specifically express Lactotransferrin and Vegfc during ear pinna regeneration in spiny mice Dev Cell 2024-01-16 [PMID: 38228141]
• Kim M, Kim WS, Cha H et Al. Early involvement of peripherally derived monocytes in inflammation in an NMO-like mouse model Sci Rep 2024-01-12 [PMID: 38216632]
• Silveira Prudente, A;Hoon Lee, S;Roh, J;Luckemeyer, DD;Cohen, CF;Pertin, M;Park, CK;Suter, MR;Decosterd, I;Zhang, JM;Ji, RR;Berta, T; Microglial STING activation alleviates nerve injury-induced neuropathic pain in male but not female mice Brain, behavior, and immunity 2024-01-06 [PMID: 38190983]
• Cui Y, Zhang Z, Lv M et Al. Chromatin target of protein arginine methyltransferases alleviates cerebral ischemia/reperfusion-induced injury by regulating RNA alternative splicing iScience 2023-12-07 [PMID: 38188517]
• Estrid Thougaard, Brianna Carney, Agnieszka Wlodarczyk, Roberta Brambilla, Kate Lykke Lambertsen Peripherally derived myeloid cells induce disease-dependent phenotypic changes in microglia Frontiers in Cellular Neuroscience 2023-12-14 [PMID: 38155863]
• Lorena Morton, Philipp Arndt, Alejandra P. Garza, Solveig Henneicke, Hendrik Mattern, Marilyn Gonzalez, Alexander Dityatev, Deniz Yilmazer-Hanke, Stefanie Schreiber, Ildiko R. Dunay Spatio-temporal dynamics of microglia phenotype in human and murine cSVD: impact of acute and chronic hypertensive states Acta Neuropathologica Communications 2023-12-19 [PMID: 38115109]
• Lindquist, KA;Shein, SA;Hovhannisyan, AH;Mecklenburg, J;Zou, Y;Lai, Z;Tumanov, AV;Akopian, AN; Associations of tissue damage induced inflammatory plasticity in masseter muscle with the resolution of chronic myalgia Scientific reports 2023-12-12 [PMID: 38086903]
• Chen Y, Song S, Parhizkar S, Lord J et Al. APOE3ch alters microglial response and suppresses A?-induced tau seeding and spread Cell 2023-12-12 [PMID: 38086389]
• Zeynep Kaya, Nevin Belder, Melike Sever-Bahcekapili, Buket Donmez-Demir, Şefik Evren Erdener, Naz Bozbeyoglu, Canan Bagci, Emine Eren-Kocak, Muge Yemisci, Hulya Karatas, Esra Erdemli, Ihsan Gursel, Turgay Dalkara Vesicular HMGB1 release from neurons stressed with spreading depolarization enables confined inflammatory signaling to astrocytes Journal of Neuroinflammation 2023-12-11 [PMID: 38082296]
• Zhai D, Yan S, Samsom J et Al. Small-molecule targeting AMPA-mediated excitotoxicity has therapeutic effects in mouse models for multiple sclerosis Sci Adv 2023-12-08 [PMID: 38064562]
• Feng T, Du H, Hu F Loss of TMEM106B exacerbates Tau pathology and neurodegeneration in PS19 mice bioRxiv : the preprint server for biology 2023-11-15 [PMID: 38014238]
• Yadong Wang, Zhaohui Dang, Xu Wang, Yuanyuan Chen, Peng Dong, Gang Liu, Weibin Tan, Zhong Gui, Fan Bu, Feng Lin, Chaozhao Liang Obacunone alleviates chronic pelvic pain and pro-inflammatory depolarization of macrophage induced by experimental autoimmune prostatitis in mice Biochemistry and Biophysics Reports 2023-10-31 [PMID: 37965064]
• Zamith Cunha R, Semprini A, Salamanca G et al. Expression of Cannabinoid Receptors in the Trigeminal Ganglion of the Horse International journal of molecular sciences 2023-11-03 [PMID: 37958932]
• Li HL, Guo RJ, Ai ZR, Han S et Al. Upregulation of Spinal MDGA1 in Rats After Nerve Injury Alters Interactions Between Neuroligin-2 and Postsynaptic Scaffolding Proteins and Increases GluR1 Subunit Surface Delivery in the Spinal Cord Dorsal Horn Neurochem Res 2023-11-13 [PMID: 37955815]
• Connolly KJ, Margaria J, Di Biase E et al. Loss of Lipid Carrier ApoE Exacerbates Brain Glial and Inflammatory Responses after Lysosomal GBA1 Inhibition Cells 2023-11-02 [PMID: 37947642] (IHC-FrFl, Mouse)
• Denaro S, Pasquinucci L, Turnaturi R et al. Sigma-1 Receptor Inhibition Reduces Mechanical Allodynia and Modulate Neuroinflammation in Chronic Neuropathic Pain Molecular neurobiology 2023-11-03 [PMID: 37922065] (IHC, Rat)
  
  Details:
  1:100 dilution
• Wu DM, Liu JP, Liu J et al. Immune pathway activation in neurons triggers neural damage after stroke Cell reports 2023-11-01 [PMID: 37917581] (Immunohistochemistry, Mouse)
• Wang S, Jiang C, Cao K et al. HK2 in microglia and macrophages contribute to the development of neuropathic pain Glia 2023-11-01 [PMID: 37909251]
• Groh J, Abdelwahab T, Kattimani Y et al. Microglia-mediated demyelination protects against CD8+ T cell-driven axon degeneration in mice carrying PLP defects Nature communications 2023-10-30 [PMID: 37903797] (Immunohistochemistry, Mouse)
• Wang JX, Xiao X, He XC et al. Agomir-331 Suppresses Reactive Gliosis and Neuroinflammation after Traumatic Brain Injury Cells 2023-10-11 [PMID: 37887272] (IHC-Fr, Mouse)
• Cao H, Li B, Mu M et al. Nicotine suppresses crystalline silica-induced astrocyte activation and neuronal death by inhibiting NF-?B in the mouse hippocampus CNS neuroscience & therapeutics 2023-10-21 [PMID: 37864452] (Immunohistochemistry, Mouse)
• M. Alejandra Zeballos C., Hayden J. Moore, Tyler J. Smith, Jackson E. Powell, Najah S. Ahsan, Sijia Zhang, Thomas Gaj Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteins Nature Communications 2023-10-14 [PMID: 37838698]
• Colvett I, Gilmore A, Guzman S et al. Recipient Reaction and Composition of Autologous Sural Nerve Tissue Grafts into the Human Brain Journal of Clinical Medicine 2023-09-22 [PMID: 37834764] (IHC-P, Human)
• Lu Z, Zhang M, Lee J et al. Cell 2023-09-28 [PMID: 37774676] (IHC-P, Human)
  
  Details:
  1:1000 IHC-P dilution
• Zamith Cunha R, Salamanca G, Mille F et al. Endocannabinoid System Receptors at the Hip and Stifle Joints of Middle-Aged Dogs: A Novel Target for the Therapeutic Use of Cannabis sativa Extract in Canine Arthropathies Animals (Basel) 2023-09-06 [PMID: 37760233]
• Cullen PF, Mazumder AG, Sun D, Flanagan JG Rapid isolation of intact retinal astrocytes: a novel approach Acta neuropathologica communications 2023-09-25 [PMID: 37749651] (IP, Mouse)
  
  Details:
  1:200 IP dilution
• Yin Z, Rosenzweig N, Kleemann KL et al. APOE4 impairs the microglial response in Alzheimer's disease by inducing TGF?-mediated checkpoints Nature immunology 2023-09-25 [PMID: 37749326]
• Stevenson DK, Murray JC, Muglia LJ et Al. Challenges to the study of gender dysphoria Acta Paediatr 2023-11-01 [PMID: 37724910]
• Holden JM, Wareham LK, Calkins DJ. Retinal astrocyte morphology predicts integration of vascular and neuronal architecture Frontiers in Neuroscience 2023-08-09 [PMID: 37621717]
• Yang X, Cao Q, Guo Y et al. GSDMD knockdown attenuates phagocytic activity of microglia and exacerbates seizure susceptibility in TLE mice Journal of Neuroinflammation 2023-08-23 [PMID: 37612735]
• Crivelli SM, Quadri Z, Vekaria HJ et al. Inhibition of acid sphingomyelinase reduces reactive astrocyte secretion of mitotoxic extracellular vesicles and improves Alzheimer's disease pathology in the 5xFAD mouse Acta Neuropathologica Communications 2023-08-21 [PMID: 37605262]
• Douglass JD, Ness KM, Valdearcos M et al. Obesity-associated microglial inflammatory activation paradoxically improves glucose tolerance Cell metabolism 2023-08-11 [PMID: 37572666]
• Sinner, P;Peckert-Maier, K;Mohammadian, H;Kuhnt, C;Dra beta ner, C;Panagiotakopoulou, V;Rauber, S;Linnerbauer, M;Haimon, Z;Royzman, D;Kronenberg-Versteeg, D;Ramming, A;Steinkasserer, A;Wild, AB; Microglial expression of CD83 governs cellular activation and restrains neuroinflammation in experimental autoimmune encephalomyelitis Nature communications 2023-08-01 [PMID: 37528070]
• Feng T, Minevich G, Liu P et al. AAV-GRN partially corrects motor deficits and ALS/FTLD-related pathology in Tmem106bGrn mice iScience 2023-06-01 [PMID: 37519899]
• Ali T, Klein AN, McDonald K et al. Cellulose ether treatment inhibits amyloid beta aggregation, neuroinflammation and cognitive deficits in transgenic mouse model of Alzheimer's disease Journal of neuroinflammation 2023-07-28 [PMID: 37507761] (ICC/IF, Mouse)
  
  Details:
  1:25 dilution
• Garcia MG, Paulus A, V�zquez-Reyes S et al. Maternal separation differentially modulates early pathology by sex in 5xFAD Alzheimer's disease-transgenic mice Brain, Behavior, & Immunity - Health 2023-10-01 [PMID: 37503358]
• Holuka C, Morel C, Roth S et al. The epigenetic hallmark of early-life ?-hexabromocyclododecane exposure: From cerebellar 6-mA levels to locomotor performance in adulthood Environment international 2023-07-20 [PMID: 37494814] (WB, Rat)
  
  Details:
  Dilution: 1:2000
• Luo X CXCR2 antagonism attenuates neuroinflammation after subarachnoid hemorrhage Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 2023-07-21 [PMID: 37481938] (IHC-P, Mouse)
• Zhu Z, Zhang L, Elsherbini A et al. The S1P receptor 1 antagonist Ponesimod reduces TLR4-induced neuroinflammation and increases A? clearance in 5XFAD mice EBioMedicine 2023-07-20 [PMID: 37480622] (IHC)
• Wu J, Li Y, Huang Y et al. Integrating spatial and single-nucleus transcriptomic data elucidates microglial-specific responses in female cynomolgus macaques with depressive-like behaviors Nature neuroscience 2023-07-13 [PMID: 37443281]
• Yang Y, García-Cruzado M, Zeng H et al. LPS priming before plaque deposition impedes microglial activation and restrains A? pathology in the 5xFAD mouse model of Alzheimer's disease Brain, behavior, and immunity 2023-07-10 [PMID: 37437821] (IHC-Fr, Mouse)
  
  Details:
  1:500 dilution
• Huang Y, Wang Z, Ye B et al. Sodium butyrate ameliorates diabetic retinopathy in mice via the regulation of gut microbiota and related short-chain fatty acids Journal of translational medicine 2023-07-07 [PMID: 37420234] (IHC-WhMt, Mouse)
• Lozano DC, Jayaram H, Cepurna WO et al. Optic Nerve Head Gene Transcription Sequelae to a Single Elevated IOP Exposure Provides Insights Into Known Responses to Chronically Elevated IOP Investigative Opthalmology & Visual Science 2023-07-05 [PMID: 37405758]
• Bu F, Li Y, Lan S et al. Blocking Pannexin-1 Channels Alleviates Thalamic Hemorrhage-Induced Pain and Inflammatory Depolarization of Microglia in Mice ACS chemical neuroscience 2023-06-28 [PMID: 37377340]
• Mo J, Lu Z, Peng J et al. PAG neuronal NMDARs activation mediated morphine-induced hyperalgesia by HMGB1-TLR4 dependent microglial inflammation Journal of psychiatric research 2023-06-15 [PMID: 37352811] (IHC)
• Cao M, Huang X, Zou J et al. Attenuation of Microglial Activation and Pyroptosis by Inhibition of P2X7 Pathway Promotes Photoreceptor Survival in Experimental Retinal Detachment Investigative ophthalmology & visual science 2023-06-01 [PMID: 37351878] (IHC-Fr, Mouse)
• Greenman R, Segal-Salto M, Barashi N et al. CCL24 regulates biliary inflammation and fibrosis in primary sclerosing cholangitis JCI insight 2023-06-22 [PMID: 37345655] (IHC-P, Mouse)
• Zheng G, Harms AK, Tail M et al. Effects of a neurokinin-1 receptor antagonist in the acute phase after thoracic spinal cord injury in a rat model Frontiers in molecular neuroscience 2023-05-12 [PMID: 37251648] (IHC-P, Mouse)
  
  Details:
  1:200 IHC-P dilution
• Jonas M, Simon AJ, Gilburd B, Schneiderman J Intrarenal Anti-Leptin Treatment Attenuates Ischemia and Reperfusion Injury American journal of nephrology 2023-05-24 [PMID: 37231766] (Immunohistochemistry)
• Abdelwahab T, Stadler D, Knöpper K et al. Cytotoxic CNS-associated T cells drive axon degeneration by targeting perturbed oligodendrocytes in PLP1 mutant mice iScience 2023-05-01 [PMID: 37182098] (IHC, Mouse)
• Choconta JL, Labi V, Dumbraveanu C et al. Age-related neuroimmune signatures in dorsal root ganglia of a Fabry disease mouse model Immunity & ageing : I & A 2023-05-12 [PMID: 37173694] (IHC, Mouse)
  
  Details:
  IHC 1:100, Antigen rerieval: sodium citrate 80C/30min
• Medina-Rodriguez EM, Han D, Lowell J, Beurel E Stress promotes the infiltration of peripheral immune cells to the brain Brain, behavior, and immunity 2023-05-09 [PMID: 37169132]
• Qi, L;Li, X;Zhang, F;Zhu, X;Zhao, Q;Yang, D;Hao, S;Li, T;Li, X;Tian, T;Feng, J;Sun, X;Wang, X;Gao, S;Wang, H;Ye, J;Cao, S;He, Y;Wang, H;Wei, B; VEGFR-3 signaling restrains the neuron-macrophage crosstalk during neurotropic viral infection Cell reports 2023-05-10 [PMID: 37167063]
• Wei, H;Wu, X;Withrow, J;Cuevas-Diaz Duran, R;Singh, S;Chaboub, LS;Rakshit, J;Mejia, J;Rolfe, A;Herrera, JJ;Horner, PJ;Wu, JQ; Glial progenitor heterogeneity and key regulators revealed by single-cell RNA sequencing provide insight to regeneration in spinal cord injury Cell reports 2023-05-06 [PMID: 37149868] (Immunocytochemistry/ Immunofluorescence)
• Tahir Ali, Antonia N Klein, Alex Vu, Maria I Arifin, Samia Hannaoui, Sabine Gilch Peptide aptamer targeting A beta -PrP-Fyn axis reduces Alzheimer's disease pathologies in 5XFAD transgenic mouse model. Cellular and molecular life sciences : CMLS 2023-05-09 [PMID: 37149826]
• Zhang, T;Pang, W;Feng, T;Guo, J;Wu, K;Nunez Santos, M;Arthanarisami, A;Nana, AL;Nguyen, Q;Kim, PJ;Jankowsky, JL;Seeley, WW;Hu, F; TMEM106B regulates microglial proliferation and survival in response to demyelination Science advances 2023-05-05 [PMID: 37146150] (WB, Mouse)
• Zhou J, Lin S, Hu Q et al. Microglial CD11b Knockout Contributes to Axonal Debris Clearance and Axonal Degradation Attenuation via IGF-1 After Acute Optic Nerve Injury Investigative ophthalmology & visual science 2023-05-01 [PMID: 37145604] (Immunohistochemistry-Frozen, Mouse)
• Qin C, Yang S, Chen M et al. Modulation of microglial metabolism facilitates regeneration in demyelination iScience 2023-05-19 [PMID: 37138776] (WB, Human)
• Lindquist KA, Shein SA, Hovhannisyan AH et al. Association of inflammation and tissue damage induced biological processes in masseter muscle with the resolution of chronic myalgia bioRxiv : the preprint server for biology 2023-04-22 [PMID: 37131723] (IHC-Fr)
• Han S, Wang Q, Song Y et al. Lithium ameliorates Niemann-Pick C1 disease phenotypes by impeding STING/SREBP2 activation iScience 2023-05-19 [PMID: 37128603]
• Zhang T, Feng T, Wu K et al. Progranulin deficiency results in sex-dependent alterations in microglia in response to demyelination Acta Neuropathologica 2023-07-01 [PMID: 37120788]
• Wang G, Yin W, Shin H Et al. Neuronal accumulation of peroxidated lipids promotes demyelination and neurodegeneration through the activation of the microglial NLRP3 inflammasome Nature Aging 2021-11-01 [PMID: 37118341]
• Janos Groh, Konrad Knöpper, Panagiota Arampatzi, Xidi Yuan, Lena Lö beta lein, Antoine-Emmanuel Saliba, Wolfgang Kastenmüller, Rudolf Martini Accumulation of cytotoxic T cells in the aged CNS leads to axon degeneration and contributes to cognitive and motor decline. Nature aging 2023-07-19 [PMID: 37117598]
• Filipello F, You SF, Mirfakhar FS et al. Defects in lysosomal function and lipid metabolism in human microglia harboring a TREM2 loss of function mutation Acta Neuropathologica 2023-06-01 [PMID: 37115208]
• Parhizkar S, Gent G, Chen Y et al. Sleep deprivation exacerbates microglial reactivity and A? deposition in a TREM2-dependent manner in mice Science translational medicine 2023-04-26 [PMID: 37099634] (Immunocytochemistry/ Immunofluorescence, Mouse)
• Lillian Yang, John H Martin Effects of motor cortex neuromodulation on the specificity of corticospinal tract spinal axon outgrowth and targeting in rats. Brain stimulation 2023-06-26 [PMID: 37094762]
• Zeballos C MA, Moore HJ, Powell JE et al. Mitigating a TDP-43 proteinopathy using RNA-targeting CRISPR effector proteins bioRxiv : the preprint server for biology 2023-04-08 [PMID: 37066174] (IHC, Mouse)
  
  Details:
  1:800 dilution
• Bo X, Xie F, Zhang J et al. Deletion of Calhm2 alleviates MPTP-induced Parkinson's disease pathology by inhibiting EFHD2-STAT3 signaling in microglia Theranostics 2023-03-13 [PMID: 37064868] (IHC-Fr, Mouse)
• Harrington EP, Catenacci RB, Smith MD et al. MHC class I and MHC class II reporter mice enable analysis of immune oligodendroglia in mouse models of multiple sclerosis eLife 2023-04-14 [PMID: 37057892]
• Shi Y, Liu Y, Wu C, Liu X et Al. N,N-Dimethyl-3?-hydroxycholenamide attenuates neuronal death and retinal inflammation in retinal ischemia/reperfusion injury by inhibiting Ninjurin 1 J Neuroinflammation 2023-04-07 [PMID: 37029422]
• Zamith Cunha R, Zannoni A, Salamanca G et al. Expression of cannabinoid (CB1 and CB2) and cannabinoid-related receptors (TRPV1, GPR55, and PPAR?) in the synovial membrane of the horse metacarpophalangeal joint Frontiers in veterinary science 2023-03-03 [PMID: 36937015] (IHC-P, Horse)
  
  Details:
  1:80 IHC-P dilution
• Vu AP, Lam D, Denney C et al. Social isolation produces a sex- and brain region-specific alteration of microglia state The European journal of neuroscience 2023-03-14 [PMID: 36918398] (IHC, Mouse)
• Ghezzi L, Bollman B, De Feo L et al. Schwann Cell Remyelination in the Multiple Sclerosis Central Nervous System Laboratory investigation; a journal of technical methods and pathology 2023-03-06 [PMID: 36889543]
• Gonz�lez-Garc�a I, Garc�a-Clav� E, Cebrian-Serrano A et al. Estradiol regulates leptin sensitivity to control feeding via hypothalamic d1 Cell metabolism 2023-03-07 [PMID: 36889283] (Immunohistochemistry-Frozen, Mouse)
• Liu C, Gao X, Shi RX et al. Microglial transglutaminase 2 deficiency causes impaired synaptic remodelling and cognitive deficits in mice Cell proliferation 2023-03-06 [PMID: 36878712] (Immunocytochemistry/ Immunofluorescence, Mouse)
• Varodayan FP, Pahng AR, Davis TD et al. Chronic ethanol induces a pro-inflammatory switch in interleukin-1? regulation of GABAergic signaling in the medial prefrontal cortex of male mice Brain, behavior, and immunity 2023-02-28 [PMID: 36863493]
• Elliya Park, Lily Yi Li, Chunsheng He, Azhar Z. Abbasi, Taksim Ahmed, Warren D. Foltz, Regan O'Flaherty, Maham Zain, Robert P. Bonin, Andrew M. Rauth, Paul E. Fraser, Jeffrey T. Henderson, Xiao Yu Wu Brain‐Penetrating and Disease Site‐Targeting Manganese Dioxide‐Polymer‐Lipid Hybrid Nanoparticles Remodel Microenvironment of Alzheimer's Disease by Regulating Multiple Pathological Pathways Advanced Science 2023-02-19 [PMID: 36808713]
• Wang Y, Sadike D, Huang B et al. Regulatory T cells alleviate myelin loss and cognitive dysfunction by regulating neuroinflammation and microglial pyroptosis via TLR4/MyD88/NF-?B pathway in LPC-induced demyelination Journal of neuroinflammation 2023-02-18 [PMID: 36803990] (IHC, Mouse)
• Zhu T, Guo J, Wu Y et al. The mechanosensitive ion channel Piezo1 modulates the migration and immune response of microglia iScience 2023-02-17 [PMID: 36798430]
• Arbat-Plana A, Bolivar S, Navarro X et al. Massive Loss of Proprioceptive Ia Synapses in Rat Spinal Motoneurons after Nerve Crush Injuries in the Postnatal Period eNeuro 2023-02-01 [PMID: 36759186] (IHC-FrFl, Rat)
• Shen H, Shi XJ, Qi L, Wang C et Al. Microglia and astrocytes mediate synapse engulfment in a MER tyrosine kinase-dependent manner after traumatic brain injury Neural Regen Res 2023-02-08 [PMID: 36751804]
• Woodburn SC, Asrat HS, Flurer JK et al. Depletion of microglial BDNF increases susceptibility to the behavioral and synaptic effects of chronic unpredictable stress Brain, behavior, and immunity 2023-01-18 [PMID: 36681359] (IHC-FrFl, Mouse)
  
  Details:
  Dilution used 1:1000
• Chen XT, Chen LP, Fan LJ et Al. Microglial P2Y12 Signaling Contributes to Cisplatin-induced Pain Hypersensitivity via IL-18-mediated Central Sensitization in the Spinal Cord J Pain 2023-05-09 [PMID: 36646400]
• van Lengerich B, Zhan L, Xia D et al. A TREM2-activating antibody with a blood-brain barrier transport vehicle enhances microglial metabolism in Alzheimer's disease models Nature neuroscience 2023-01-12 [PMID: 36635496] (IHC-FrFl, Mouse)
  
  Details:
  right hemibrains, Dilution used 1:500
• Dittmann NL, Torabi P, Watson AES, Yuzwa SA et Al. Culture Protocol and Transcriptomic Analysis of Murine SVZ NPCs and OPCs Stem Cell Rev Rep 2023-01-08 [PMID: 36617597]
• Elise Marsan, Dmitry Velmeshev, Arren Ramsey, Ravi K. Patel, Jiasheng Zhang, Mark Koontz, Madeline G. Andrews, Martina de Majo, Cristina Mora, Jessica Blumenfeld, Alissa N. Li, Salvatore Spina, Lea T. Grinberg, William W. Seeley, Bruce L. Miller, Erik M. Ullian, Matthew F. Krummel, Arnold R. Kriegstein, Eric J. Huang Astroglial toxicity promotes synaptic degeneration in the thalamocortical circuit in frontotemporal dementia with GRN mutations The Journal of Clinical Investigation 2023-03-15 [PMID: 36602862]
• Gupta D, Lee Y, Choe Y et al. Knee osteoarthritis accelerates amyloid beta deposition and neurodegeneration in a mouse model of Alzheimer�s disease Molecular Brain 2023-01-02 [PMID: 36593507] (IHC, Mouse)
• Yoo JY, Kim HB, Lee YJ et al. Neuregulin-1 reverses anxiety-like behavior and social behavior deficits induced by unilateral micro-injection of CoCl2 into the ventral hippocampus (vHPC) Neurobiology of disease 2022-12-30 [PMID: 36592864] (IHC-Fr, Mouse)
  
  Details:
  Dilution used in IHC-Fr and ICC/IF 1:100. Dilution used in WB 1:1000
• Kong C, Yang EJ, Shin J et al. Enhanced delivery of a low dose of aducanumab via FUS in 5 x FAD mice, an AD model Translational neurodegeneration 2022-12-27 [PMID: 36575534] (IHC-FrFl, Mouse)
• Kong C, Yang E, Shin J et al. Enhanced delivery of low-dose of aducanumab via FUS in 5xFAD mice, an AD model Research Square 2022-08-02 [PMID: 36575534] (IHC-Fr, Mouse)
• Hu, Y;Cao, K;Wang, F;Wu, W;Mai, W;Qiu, L;Luo, Y;Ge, WP;Sun, B;Shi, L;Zhu, J;Zhang, J;Wu, Z;Xie, Y;Duan, S;Gao, Z; Dual roles of hexokinase 2 in shaping microglial function by gating glycolytic flux and mitochondrial activity Nature metabolism 2022-12-01 [PMID: 36536134]
• Daura E, Tegelberg S, Hakala P, Lehesjoki AE et Al. Cystatin B deficiency results in sustained histone H3 tail cleavage in postnatal mouse brain mediated by increased chromatin-associated cathepsin L activity Front Mol Neurosci 2022-12-19 [PMID: 36533126]
• Lin PB, Tsai AP, Soni D et al. INPP5D deficiency attenuates amyloid pathology in a mouse model of Alzheimer's disease Alzheimer's & dementia : the journal of the Alzheimer's Association 2022-12-16 [PMID: 36524682] (ICC/IF, IHC-Fr, Mouse)
  
  Details:
  Dilution used in IHC-Fr, ICC 1:500
• Ho E, Deng Y, Akbar D et al. Tunable Surface Charge Enables the Electrostatic Adsorption-Controlled Release of Neuroprotective Peptides from a Hydrogel-Nanoparticle Drug Delivery System ACS applied materials & interfaces 2022-12-15 [PMID: 36520607]
• Zhao XF, Huffman LD, Hafner H et al. The injured sciatic nerve atlas (iSNAT), insights into the cellular and molecular basis of neural tissue degeneration and regeneration eLife 2022-12-14 [PMID: 36515985]
• Zia S, Hammond BP, Zirngibl M et al. Single-cell microglial transcriptomics during demyelination defines a microglial state required for lytic carcass clearance Molecular neurodegeneration 2022-12-13 [PMID: 36514132] (IHC-Fr, Mouse)
• Kim JH, Kang RJ, Hyeon SJ et al. Lipocalin-2 Is a Key Regulator of Neuroinflammation in Secondary Traumatic and Ischemic Brain Injury Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2022-12-12 [PMID: 36508119]
• Cramer SW, Haley SP, Popa LS et al. Wide-field calcium imaging reveals widespread changes in cortical functional connectivity following mild traumatic brain injury in the mouse Neurobiology of disease 2023-01-01 [PMID: 36476979] (IHC, Mouse)
  
  Details:
  Dilution used in IHC 1:1000
• TK Kim, EJ Bae, BC Jung, M Choi, SJ Shin, SJ Park, JT Kim, MK Jung, A Ulusoy, MY Song, JS Lee, HJ Lee, DA Di Monte, SJ Lee Inflammation promotes synucleinopathy propagation Experimental & Molecular Medicine, 2022-12-06;0(0):. 2022-12-06 [PMID: 36473937]
• Youtong H, Greg L Adaptable toolbox to characterize Alzheimer’s disease pathology in mouse models STAR Protocols 2022-12-01 [PMID: 36472212] (IF/IHC, Mouse)
• Pantazis CB, Yang A, Lara E et al. A reference human induced pluripotent stem cell line for large-scale collaborative studies Cell Stem Cell 2022-12-01 [PMID: 36459969] (WB)
• Yuan P, Zhang M, Tong L et al. PLD3 affects axonal spheroids and network defects in Alzheimer's disease Nature 2022-11-30 [PMID: 36450991] (Mouse)
• Ceasrine AM, Devlin BA, Bolton JL et al. Maternal diet disrupts the placenta-brain axis in a sex-specific manner Nature Metabolism 2022-11-28 [PMID: 36443520]
• Lu H, Lin C, Clendaniel V et al. Histone Deacetylase 3 Inhibition Decreases Cerebral Edema and Protects the Blood-Brain Barrier after Stroke Research Square Mar 16 2022 (IHC, Rat)
• Gratuze M, Schlachetzki JCM, D'Oliveira Albanus R et al. TREM2-independent microgliosis promotes tau-mediated neurodegeneration in the presence of ApoE4 Neuron 2022-11-04 [PMID: 36368315] (IHC-Fr, Mouse)
  
  Details:
  Dilution used in WB 1:1000
• Safaee F ASSESSING EAR PINNA REPAIR IN THE MRL/MpJ MOUSE STRAIN Thesis Jan 1 2022 (IHC-P, Mouse)
• Lee E, Jung Y, Park Y et al. A distinct astrocyte subtype in the aging mouse brain characterized by impaired protein homeostasis Nature Aging Aug 1 2022 (IHC-Fr, Mouse)
  
  Details:
  Dilution used for IHC 1:500
• , , , et Al. Erratum regarding previously published articles Brain Hemorrhages 2022-11-21 [PMID: 36406202]
• Wood JPM, Chidlow G, Halliday LA et al. Histochemical Comparison of Human and Rat Lacrimal Glands: Implications for Bio-Engineering Studies Translational vision science & technology 2022-11-01 [PMID: 36374486] (IHC-P, Human, Rat)
  
  Details:
  Dilution used in IHC-P 1:20,000
• Jangjoo Ghalat N, Shahpasand K, Javan M Cis-p-tau plays crucial role in lysolecithin-induced demyelination and subsequent axonopathy in mouse optic chiasm Experimental neurology 2023-01-01 [PMID: 36343678] (IHC-Fr, Mouse)
  
  Details:
  Dilution used in IHC-Fr 1:100
• Xu H, You M, Xiang X et al. Molecular Mechanism of Epimedium Extract against Ischemic Stroke Based on Network Pharmacology and Experimental Validation Oxidative Medicine and Cellular Longevity 2022-10-27 [PMID: 36338345] (IF/IHC, Rat)
• Rebejac J, Eme-Scolan E, Arnaud Paroutaud L et al. Meningeal macrophages protect against viral neuroinfection Immunity 2022-10-27 [PMID: 36323311] (IHC-WhMt, Mouse)
• Lu H, Ashiqueali R, Lin CI et al. Histone Deacetylase 3 Inhibition Decreases Cerebral Edema and Protects the Blood-Brain Barrier After Stroke Molecular Neurobiology 2023-01-01 [PMID: 36258136]
• Xu XJ, Ge QQ, Yang MS et al. Neutrophil-derived interleukin-17A participates in neuroinflammation induced by traumatic brain injury Neural regeneration research 2023-05-01 [PMID: 36254991] (IHC-Fr, Mouse)
• Vilallongue N, Schaeffer J, Hesse AM et al. Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system Nature communications 2022-10-13 [PMID: 36229455] (IHC-Fr, Mouse)
• Alexandre G. Maganin, Guilherme R. Souza, Miriam D. Fonseca, Alexandre H. Lopes, Rafaela M. Guimarães, André Dagostin, Nerry T. Cecilio, Atlante S. Mendes, William A. Gonçalves, Conceição E.A. Silva, Francisco Isaac Fernandes Gomes, Lucas M. Mauriz Marques, Rangel L. Silva, Letícia M. Arruda, Denis A. Santana, Henrique Lemos, Lei Huang, Marcela Davoli-Ferreira, Danielle Santana-Coelho, Morena B. Sant’Anna, Ricardo Kusuda, Jhimmy Talbot, Gabriela Pacholczyk, Gabriela A. Buqui, Norberto P. Lopes, Jose C. Alves-Filho, Ricardo M. Leão, Jason C. O’Connor, Fernando Q. Cunha, Andrew Mellor, Thiago M. Cunha Meningeal dendritic cells drive neuropathic pain through elevation of the kynurenine metabolic pathway in mice The Journal of Clinical Investigation 2022-12-01 [PMID: 36227694]
• Leng L, Yuan Z, Pan R et al. Microglial hexokinase 2 deficiency increases ATP generation through lipid metabolism leading to B-amyloid clearance Nature metabolism 2022-10-01 [PMID: 36203054] (IHC-P, Human)
• Chen X, Liu X, Koundal S et al. Cerebral amyloid angiopathy is associated with glymphatic transport reduction and time-delayed solute drainage along the neck arteries Nature Aging 2022-03-01 [PMID: 36199752] (IF/IHC, Rat)
• Huang Y, Lemke G Early death in a mouse model of Alzheimer's disease exacerbated by microglial loss of TAM receptor signaling Proceedings of the National Academy of Sciences of the United States of America 2022-10-11 [PMID: 36191221]
• Chiocchetti R, Salamanca G, De Silva M et al. Cannabinoid receptors in the inflammatory cells of canine atopic dermatitis Frontiers in Veterinary Science 2022-09-15 [PMID: 36187821]
• Bretheau F, Castellanos-Molina A, B�langer D et al. The alarmin interleukin-1? triggers secondary degeneration through reactive astrocytes and endothelium after spinal cord injury Nature Communications 2022-10-02 [PMID: 36184639]
• Jana A, Wang X, Leasure JW et al. Increased Type I interferon signaling and brain endothelial barrier dysfunction in an experimental model of Alzheimer's disease Scientific reports 2022-10-01 [PMID: 36182964] (IHC-Fr, Mouse)
• van Ineveld RL, Collot R, Román MB, Pagliaro A et Al. Multispectral confocal 3D imaging of intact healthy and tumor tissue using mLSR-3D Nat Protoc 2022-09-30 [PMID: 36180532]
• Cooper S, Souza L, Worker C et al. Renin-a in the Subfornical Organ Plays a Critical Role in the Maintenance of Salt-Sensitive Hypertension Biomolecules 2022-08-24 [PMID: 36139008] (IHC-P, Mouse)
• He BD, Liu CM, Teng ZQ. A Mouse Model of Neurodegeneration Induced by Blade Penetrating Stab Wound to the Hippocampus Biology (Basel) 2022-09-18 [PMID: 36138848]
• Cserép C, Schwarcz AD, Pósfai B, László ZI et Al. Microglial control of neuronal development via somatic purinergic junctions Cell Rep 2022-09-21 [PMID: 36130488]
• Re GF, Li H, Yang JQ et al. Exercise modulates central and peripheral inflammatory responses and ameliorates methamphetamine-induced anxiety-like symptoms in mice Frontiers in Molecular Neuroscience 2022-08-29 [PMID: 36106141]
• Yao J, Wang T, Jia L et al. Loss of Fas Receptor Function Preserves Photoreceptor Structure and Function in Two Mouse Models of Inherited Retinal Degeneration Investigative Opthalmology & Visual Science 2022-09-09 [PMID: 36083588]
• H Shi, Z Yin, Y Koronyo, DT Fuchs, J Sheyn, MR Davis, JW Wilson, MA Margeta, KM Pitts, S Herron, S Ikezu, T Ikezu, SL Graham, VK Gupta, KL Black, M Mirzaei, O Butovsky, M Koronyo-Ha Regulating microglial miR-155 transcriptional phenotype alleviates Alzheimer&#039;s-induced retinal vasculopathy by limiting Clec7a/Galectin-3+ neurodegenerative microglia Acta neuropathologica communications, 2022-09-08;10(1):136. 2022-09-08 [PMID: 36076283]
• Bocharova OV, Fisher A, Pandit NP et al. A? plaques do not protect against HSV-1 infection in a mouse model of familial Alzheimer's disease, and HSV-1 does not induce A? pathology in a model of late onset Alzheimer's disease Brain Pathology 2023-01-01 [PMID: 36064300]
• Jang D, Yang B, You M et al. Fluoxetine decreases phagocytic function via REV-ERB alpha in microglia Research Square 2022-07-05 [PMID: 36048349]
• Cui ZQ, Hu XY, Yang T et al. TMEM16F may be a new therapeutic target for Alzheimer's disease Neural regeneration research 2023-03-01 [PMID: 36018189] (IHC-P, Mouse)
  
  Details:
  1:100 IHC-P
• Saleh B, Srikanth KD, Sneh T et al. FAK-Mediated Signaling Controls Amyloid Beta Overload, Learning and Memory Deficits in a Mouse Model of Alzheimer's Disease International journal of molecular sciences 2022-08-13 [PMID: 36012331] (IHC-P, Mouse)
• Schwarz MM, Price DA, Ganaie SS et al. Oropouche orthobunyavirus infection is mediated by the cellular host factor Lrp1 Proceedings of the National Academy of Sciences of the United States of America 2022-08-16 [PMID: 35939689] (IHC-Fr, Mouse)
  
  Details:
  Dilutions: 1:200
• Yue J, Xu R, Yin C et al. Negative effects of brain regulatory T cells depletion on epilepsy Progress in neurobiology 2022-08-02 [PMID: 35931355] (IHC-P)
  
  Details:
  Dilutions: 1:200
• Schacke S, Kirkpatrick J, Stocksdale A et al. Ezrin deficiency triggers glial fibrillary acidic protein upregulation and a distinct reactive astrocyte phenotype Glia 2022-08-05 [PMID: 35929192] (IHC-FrFl, IHC-Fr, Mouse)
  
  Details:
  Dilution used 1:500
• Jung H, Lee SY, Lim S et al. Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein Nature medicine 2022-08-04 [PMID: 35927581] (IF/IHC, Mouse)
• Block CL, Eroglu O, Mague SD et al. Prenatal environmental stressors impair postnatal microglia function and adult behavior in males Cell reports 2022-08-02 [PMID: 35926455]
• Leggio L, L'Episcopo F, Magri A et al. Small Extracellular Vesicles Secreted by Nigrostriatal Astrocytes Rescue Cell Death and Preserve Mitochondrial Function in Parkinson's Disease Advanced healthcare materials 2022-07-20 [PMID: 35856921] (ICC, Mouse)
• Schrader JM, Stanisavljevic A, Xu F, Van Nostrand WE Distinct Brain Proteomic Signatures in Cerebral Small Vessel Disease Rat Models of Hypertension and Cerebral Amyloid Angiopathy Journal of neuropathology and experimental neurology 2022-07-20 [PMID: 35856898]
• Hu X, Huang J, Li Y et al. TAZ Induces Migration of Microglia and Promotes Neurological Recovery After Spinal Cord Injury Frontiers in Pharmacology 2022-06-27 [PMID: 35833021]
• Parmigiani E, Ivanek R, Rolando C et al. Interferon-gamma resistance and immune evasion in glioma develop via Notch-regulated co-evolution of malignant and immune cells Developmental cell 2022-06-30 [PMID: 35803280] (IHC-Fr, Mouse)
• Shin H, Lee K, Kang J et al. Perampanel Reduces Brain Damage via Induction of M2 Microglia in a Neonatal Rat Stroke Model International Journal of Nanomedicine 2022-06-01 [PMID: 35782016] (WB, Rat)
• Steudler J, Ecott T, Ivan DC et al. Autoimmune neuroinflammation triggers mitochondrial oxidation in oligodendrocytes Glia 2022-06-28 [PMID: 35762739] (IHC-Fr, Mouse)
• Vicario N, Denaro S, Turnaturi R et al. Mu and Delta Opioid Receptor Targeting Reduces Connexin 43-Based Heterocellular Coupling during Neuropathic Pain International Journal of Molecular Sciences 2022-05-24 [PMID: 35682543]
• Ali Moussa HY, Manaph N, Ali G et al. Single Extracellular Vesicle Analysis Using Flow Cytometry for Neurological Disorder Biomarkers Frontiers in integrative neuroscience 2022-05-17 [PMID: 35655952]
  
  Details:
  Dilutions: 1:1000
• Uff CEG, Patel K, Yeung C, Yip PK Advances in Visualizing Microglial Cells in Human Central Nervous System Tissue Biomolecules 2022-04-19 [PMID: 35625531] (IHC-P, Human)
  
  Details:
  1:500 dilution
• George AJ, Dong B, Lail H et al. The E3 ubiquitin ligase RNF216/TRIAD3 is a key coordinator of the hypothalamic-pituitary-gonadal axis iScience 2022-06-17 [PMID: 35620441]
• Mike JK, Wu KY, White Y et al. Defining Longer-Term Outcomes in an Ovine Model of Moderate Perinatal Hypoxia-Ischemia Developmental Neuroscience 2022-05-19 [PMID: 35588703]
• Sudwarts A, Ramesha S, Gao T et al. BIN1 is a key regulator of proinflammatory and neurodegeneration-related activation in microglia Molecular neurodegeneration 2022-05-07 [PMID: 35526014] (IHC-Fr, Mouse)
• Van Broeckhoven J, Erens C, Sommer D et al. Macrophage-based delivery of interleukin-13 improves functional and histopathological outcomes following spinal cord injury Journal of Neuroinflammation 2022-12-01 [PMID: 35488301] (IF/IHC, Mouse)
• Wang YY, Deng YS, Dai SK et al. Loss of microglial EED impairs synapse density, learning, and memory Molecular psychiatry 2022-04-28 [PMID: 35484239] (IF/IHC, Mouse)
• Morel C, Christophe A, Maguin-GatE K et al. Head-to-Head Study of Developmental Neurotoxicity and Resultant Phenotype in Rats: alpha-Hexabromocyclododecane versus Valproic Acid, a Recognized Model of Reference for Autism Spectrum Disorders Toxics 2022-04-06 [PMID: 35448441] (WB, Rat)
• Roy ER, Chiu G, Li S et al. Concerted type I interferon signaling in microglia and neural cells promotes memory impairment associated with amyloid ? plaques Immunity 2022-05-01 [PMID: 35443157]
• Morikawa S, Blacher L, Onwumere C, Urano F Loss of Function of WFS1 Causes ER Stress-Mediated Inflammation in Pancreatic Beta-Cells Frontiers in endocrinology 2022-03-25 [PMID: 35399956] (IF/IHC, Mouse)
• Zhao Q, Dai W, Chen HY et al. Prenatal disruption of blood-brain barrier formation via cyclooxygenase activation leads to lifelong brain inflammation Proceedings of the National Academy of Sciences of the United States of America 2022-04-12 [PMID: 35377817] (IF/IHC, Mouse)
• Stanisavljevic, A;Schrader, J;Zhu, X;Mattar, J;Hanks, A;Xu, F;Majchrzak, M;Robinson, J;Van Nostrand, W; Impact of Non-pharmacological Chronic Hypertension on a Transgenic Rat Model of Cerebral Amyloid Angiopathy Frontiers in Neuroscience [PMID: 35368255] (IF/IHC, Rat)
• Shui M, Sun Y, Lin D et al. Anomalous Levels of CD47/Signal Regulatory Protein Alpha in the Hippocampus Lead to Excess Microglial Engulfment in Mouse Model of Perioperative Neurocognitive Disorders Frontiers in Neuroscience 2022-03-11 [PMID: 35360151] (IF/IHC, Mouse)
• Marrone L, Marchi PM, Webster CP et al. SPG15 protein deficits are at the crossroads between lysosomal abnormalities, altered lipid metabolism and synaptic dysfunction Human molecular genetics 2022-03-21 [PMID: 35313342] (IHC-Fr, Mouse)
• Pan Ry, He L, Zhang J et al. Positive feedback regulation of microglial glucose metabolism by histone H4 lysine 12 lactylation in Alzheimer's disease Cell metabolism 2022-03-11 [PMID: 35303422]
• Feng, T;Luan, L;Katz, II;Ullah, M;Van Deerlin, VM;Trojanowski, JQ;Lee, EB;Hu, F; TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss Acta neuropathologica communications [PMID: 35287730] (Mouse)
• Tsai AP, Dong C, Lin PB et al. PLCG2 is associated with the inflammatory response and is induced by amyloid plaques in Alzheimer's disease Genome medicine 2022-02-18 [PMID: 35180881] (IHC-FrFl, Mouse)
• Du H, Zhou X, Feng T, Hu F Regulation of lysosomal trafficking of progranulin by sortilin and prosaposin Brain Communications 2022-01-04 [PMID: 35169707] (IF/IHC, Mouse)
• Huang F, Liu X, Xu S et al. Prevotella histicola Mitigated Estrogen Deficiency-Induced Depression via Gut Microbiota-Dependent Modulation of Inflammation in Ovariectomized Mice Frontiers in Nutrition 2022-01-26 [PMID: 35155523]
• Xia L, Liu L, Cai Y et al. Inhibition of Gasdermin D-Mediated Pyroptosis Attenuates the Severity of Seizures and Astroglial Damage in Kainic Acid-Induced Epileptic Mice Frontiers in Pharmacology 2022-01-28 [PMID: 35153737]
• Gentry N, McMahon T, Yamazaki M et al. Microglia are involved in the protection of memories formed during sleep deprivation Neurobiology of Sleep and Circadian Rhythms 2022-05-01 [PMID: 35028489] (IF/IHC, Mouse)
• Sipione S, Galleguillos D, Wang Q et al. Anti-inflammatory Role of GM1 and Other Gangliosides on Microglia J Neuroinflammation 2022-01-07 [PMID: 34991625]
• Liu J, Ma W, Zang CH et al. Salidroside inhibits NLRP3 inflammasome activation and apoptosis in microglia induced by cerebral ischemia/reperfusion injury by inhibiting the TLR4/NF-?B signaling pathway Annals of Translational Medicine 2021-11-01 [PMID: 34988203]
• Wang K, Xu F, Maylie J, Xu J Anti-MUllerian Hormone Regulation of Synaptic Transmission in the Hippocampus Requires MAPK Signaling and Kv4.2 Potassium Channel Activity Frontiers in Neuroscience 2021-12-16 [PMID: 34975379] (IF/IHC, Mouse)
• Patania OM, Chiba S, Halfmann PJ et al. Pulmonary lesions induced by SARS-CoV-2 infection in domestic cats Veterinary pathology 2021-12-29 [PMID: 34963403] (IF/IHC, Feline)
• Viatchenko-Karpinski V, Kong L, Weng HR Activation of microglial GPR109A alleviates thermal hyperalgesia in female lupus mice by suppressing IL-18 and glutamatergic synaptic activity Glia 2021-12-16 [PMID: 34919284]
• Schrader JM, Xu F, Lee H et al. Emergent White Matter Degeneration in the rTg-DI Rat Model of Cerebral Amyloid Angiopathy Exhibits Unique Proteomic Changes The American journal of pathology 2021-12-08 [PMID: 34896071] (IHC-Fr, Rat)
• M Di Filippo, A Mancini, L Bellingacc, L Gaetani, P Mazzocchet, T Zelante, L La Barbera, A De Luca, M Tantucci, A Tozzi, V Durante, M Sciaccalug, A Megaro, D Chiasserin, N Salvadori, V Lisetti, E Portaccio, C Costa, P Sarchielli, MP Amato, L Parnetti, MT Viscomi, L Romani, P Calabresi Interleukin-17 affects synaptic plasticity and cognition in an experimental model of multiple sclerosis Cell Reports, 2021-12-07;37(10):110094. 2021-12-07 [PMID: 34879272]
• Lin J, Zhou L, Luo Z et al. Flow cytometry analysis of immune and glial cells in a trigeminal neuralgia rat model Scientific reports 2021-12-07 [PMID: 34876649] (IHC-Fr, Rat)
• Manigat LC, Granade ME, Taori S Et al. Loss of Diacylglycerol Kinase alpha Enhances Macrophage Responsiveness Frontiers in immunology 2021-11-05 [PMID: 34804012] (IF/IHC, Mouse)
• Cohen CF, Prudente AS, Berta T, Lee SH. Transient Receptor Potential Channel 4 Small-Molecule Inhibition Alleviates Migraine-Like Behavior in Mice Frontiers in Molecular Neuroscience 2021-11-01 [PMID: 34790097]
• Jang JH, Kim HK, Seo DW et al. Whole-Brain Mapping of the Expression Pattern of T1R2, a Subunit Specific to the Sweet Taste Receptor Frontiers in Neuroanatomy 2021-10-28 [PMID: 34776881]
• Talley S, Valiauga R, Anderson L Et al. DSS-induced inflammation in the colon drives a proinflammatory signature in the brain that is ameliorated by prophylactic treatment with the S100A9 inhibitor paquinimod Journal of neuroinflammation 2021-11-10 [PMID: 34758843] (IF/IHC, Mouse)
• Jin L, Xiao L, Ding M Et al. Heterogeneous macrophages contribute to the pathology of disc herniation induced radiculopathy The spine journal : official journal of the North American Spine Society 2021-10-27 [PMID: 34718176] (IF/IHC, Human)
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  Details:
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• Stanojlovic M, Pallais JP, Kotz CM Chemogenetic Modulation of Orexin Neurons Reverses Changes in Anxiety and Locomotor Activity in the A53T Mouse Model of Parkinson's Disease Front Neurosci 2019-07-30 [PMID: 31417337] (IF/IHC, Mouse)
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• Serra MP, Boi M, Poddighe L, Melis T et al. Resveratrol Regulates BDNF, trkB, PSA-NCAM, and Arc Expression in the Rat Cerebral Cortex after Bilateral Common Carotid Artery Occlusion and Reperfusion Nutrients 2019-05-01 [PMID: 31052460] (IHC-Fr, Rat)
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• Boisvert, EM;Means, RE;Michaud, M;Madri, JA;Katz, SG; Minocycline mitigates the effect of neonatal hypoxic insult on human brain organoids Cell Death Dis 2019-04-11 [PMID: 30975982] (IF/IHC, Human)
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• Souza, LA;Worker, CJ;Li, W;Trebak, F;Watkins, T;Gayban, AJB;Yamasaki, E;Cooper, SG;Drumm, BT;Feng, Y; The (Pro)renin Receptor Knockdown in the Paraventricular Nucleus of Hypothalamus Attenuates Hypertension Development and AT1 Receptor-Mediated Calcium Events Am. J. Physiol. Heart Circ. Physiol. 2019-03-29 [PMID: 30925093] (ICC/IF, Mouse)
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• Lenz KM, Pickett LA, Wright CL et al. Mast Cells in the Developing Brain Determine Adult Sexual Behavior. bioRxiv. [PMID: 30093566] (IF/IHC, Rat)
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• Qureshi YH, Patel VM, Berman DE et al. An Alzheimer's linked loss-of-function CLN5 variant impairs Cathepsin D maturation consistent with a retromer trafficking defect. Mol. Cell. Biol. 2018-07-23 [PMID: 30037983]
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• Li J, Serafin E, Baccei ML. Prostaglandin Signaling Governs Spike Timing-Dependent Plasticity at Sensory Synapses onto Mouse Spinal Projection Neurons. J. Neurosci. 2018-07-25 [PMID: 29934349] (IF/IHC, Mouse)
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• Hou Y, Lautrup S, Cordonnier S et al. NAD+ supplementation normalizes key Alzheimer's features and DNA damage responses in a new AD mouse model with introduced DNA repair deficiency Proc. Natl. Acad. Sci. U.S.A. 2018-02-20 [PMID: 29432159] (ICC/IF, Mouse)
• Zhang R, Wu Y, Xie F, Zhong Y. RGMa mediates reactive astrogliosis and glial scar formation through TGFb1/Smad2/3 signaling after stroke. Cell Death Differ. 2018-02-02 [PMID: 29396549] (ICC/IF, Rat)
• Bergholt M S, Serio A et al. Correlated Heterospectral Lipidomics for Biomolecular Profiling of Remyelination in Multiple Sclerosis. ACS Cent Sci 2018-01-24 [PMID: 29392175] (IF/IHC, Mouse)
• Cooper SG, Trivedi DP, Yamamoto R et al. Increased (Pro) renin Receptor Expression in Subfornical Organ Neurons in Hypertensive Humans. American Journal of Physiology-Heart and Circulatory Physiology 2017-12-22 [PMID: 29351470] (ICC/IF, Human)
• Chang LH, Lin HC, Huang SS et al. Blockade of soluble epoxide hydrolase attenuates post-ischemic neuronal hyperexcitation and confers resilience against stroke with TrkB activation Sci Rep 2018-01-08 [PMID: 29311641] (IHC-Fr, Mouse)
• Marques-Torrejon MA, Gangoso E, Pollard SM. Modelling glioblastoma tumour-host cell interactions using adult brain organotypic slice co-culture. Dis Model Mech 2017-12-01 [PMID: 29196443] (Mouse)
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• Jiang J, Luo Y, Qin W et al. Electroacupuncture Suppresses the NF-kB Signaling Pathway by Upregulating Cylindromatosis to Alleviate Inflammatory Injury in Cerebral Ischemia/Reperfusion Rats Front Mol Neurosci. 2017-11-06 [PMID: 29163038] (ICC/IF, Rat)
• Hansruedi Mathys, Chinnakkaruppan Adaikkan, Fan Gao, Jennie Z. Young, Elodie Manet, Martin Hemberg, Philip L. De Jager, Richard M. Ransohoff, Aviv Regev, Li-Huei Tsai Temporal Tracking of Microglia Activation in Neurodegeneration at Single-Cell Resolution Cell reports 2017-10-06 [PMID: 29020624]
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• Kuboyama T, Wahane S, Huang Y et al. HDAC3 inhibition ameliorates spinal cord injury by immunomodulation Sci Rep 2017-08-17 [PMID: 28819194] (IF/IHC, Mouse)
• Liu J, Li C, Peng H et al. Electroacupuncture attenuates learning and memory impairment via activation of alpha 7nAChR-mediated anti-inflammatory activity in focal cerebral ischemia/reperfusion injured rats Exp Ther Med. 2017-06-15 [PMID: 28810545] (IHC-P, Rat)
• Mei Z, Zheng P, Tan X et al. Huperzine A alleviates neuroinflammation, oxidative stress and improves cognitive function after repetitive traumatic brain injury Metab Brain Dis 2017-07-26 [PMID: 28748496] (IHC-F, Mouse)
• Langseth AJ, Kim J, Ugolino JE et al. Cell-type specific differences in promoter activity of the ALS-linked C9orf72 mouse ortholog Sci Rep 2017-07-18 [PMID: 28720882] (IHC-Fr, Mouse)
• Wohleb ES, Terwilliger R, Duman CH, Duman RS. Stress-Induced Neuronal Colony Stimulating Factor 1 Provokes Microglia-Mediated Neuronal Remodeling and Depressive-like Behavior. Biol Psychiatry 2018-01-01 [PMID: 28697890]
• Choi JY, Hwang CJ, Lee HP et al. Inhibitory effect of ethanol extract of Nannochloropsis oceanica on lipopolysaccharide-induced neuroinflammation, oxidative stress, amyloidogenesis and memory impairment Oncotarget 2017-07-11 [PMID: 28489589] (IHC-F, Mouse)
• Kwon Sun-Ho, Han Jeong-Kyu, Choi Moonseok et al. Dysfunction of Microglial STAT3 Alleviates Depressive Behavior via Neuron-Microglia Interactions. Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology 2017-01-01 [PMID: 28480882] (IF/IHC, WB, Mouse)
• Xiao J, Yao J, Jia L et al. Protective Effect of Met12, a Small Peptide Inhibitor of Fas, on the Retinal Pigment Epithelium and Photoreceptor After Sodium Iodate Injury. Invest. Ophthalmol. Vis. Sci. 2017-03-01 [PMID: 28346613]
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• Theus MH, Sparks JB, Liao X et al. All-Trans-Retinoic Acid Augments the Histopathological Outcome of Neuroinflammation and Neurodegeneration in Lupus-Prone MRL/lpr Mice. J. Histochem. Cytochem. 2016-11-16 [PMID: 27856824] (IF/IHC, Mouse)
• Roche SL, Wyse-Jackson AC, Gomez-Vicente V et al. Progesterone Attenuates Microglial-Driven Retinal Degeneration and Stimulates Protective Fractalkine-CX3CR1 Signaling. PLoS ONE. 2016-11-04 [PMID: 27814376] (Mouse)
• Healy S, McMahon J et al. Significant glial alterations in response to iron loading in a novel organotypic hippocampal slice culture model. Sci Rep 2016-03-11 [PMID: 27808258] (IF/IHC, Rat)
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• Rodhe J, Burguillos MA, de Pablos RM et al. Spatio-temporal activation of caspase-8 in myeloid cells upon ischemic stroke. Acta Neuropathol Commun. 2016-08-27 [PMID: 27566702] (IF/IHC, ICC/IF, Mouse)
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• Choi M, Ahn S, Yang EJ et al. Hippocampus-based contextual memory alters the morphological characteristics of astrocytes in the dentate gyrus. Mol Brain 2016-07-26 [PMID: 27460927] (WB)
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• Solomonov Y, Hadad N, Levy R. Reduction of cytosolic phospholipase A2 alpha upregulation delays the onset of symptoms in SOD1G93A mouse model of amyotrophic lateral sclerosis. J Neuroinflammation 2016-06-02 [PMID: 27250836] (IHC-Fr)
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• Chakravarthy H, Navitskaya S, O'Reilly S et al. Role of acid sphingomyelinase in shifting the balance between pro-inflammatory and reparative bone marrow cells in diabetic retinopathy. Stem Cells. 2015-12-16 [PMID: 26676316] (IHC-WhMt, Mouse)
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  Details:
  AIF-1/Iba1 antibody was used for IHC-Fr application on rat brain in experiments involving prechiasmatic injection subarachnoid hemorrhage /SAH model. IHC-Fr assay involved - Immersion of brain tissues in in 4 % PFA for ON and then in 20 % saccharose PBS for 72 hours followed by 30 % saccharose PBS for 72 hours, 6 umm cryosections, use of primary antibody at 1:20 dilution with 4C ON incubation, detection using fluorescence dye-conjugated secondary antibodies and DAPI (Fig. 6, Fig. 12)
• Cote M, Poirier AA, Aube B et al. Partial depletion of the proinflammatory monocyte population is neuroprotective in the myenteric plexus but not in the basal ganglia in a MPTP mouse model of Parkinson's disease Brain Behav. Immun. 2015-01-27 [PMID: 25637482] (IF/IHC, Mouse)
• Sagy-Bross C, Kasianov K, Solomonov Y et al. The role of Cytosolic Phospholipase A2 alpha in Amyloid Precursor Protein induction by amyloid beta1-42 - Implication for Neurodegeneration. J. Neurochem. 2014-12-23 [PMID: 25533654] (WB, Mouse)
• Lee S, Nam Y, Koo Jy et al. A small molecule binding HMGB1 and HMGB2 inhibits microglia-mediated neuroinflammation. Nat. Chem. Biol. 2014-10-12 [PMID: 25306442] (IF/IHC, Mouse)
• Kirkley KS, Madl JE, Duncan C, Gulland FM. Domoic acid-induced seizures in California sea lions (Zalophus californianus) are associated with neuroinflammatory brain injury. Aquatic Toxicology. 2014-09-16 [PMID: 25286249]
• Friedel R, Huang Y, Wang M Et al. Regulation of cell distancing in peri-plaque glial nets by Plexin-B1 affects glial activation and amyloid compaction in Alzheimer s disease Research Square 2021-10-28 (ICC/IF, Mouse)
• Goh MJ Evaluation of a Potential Mechanism of Sudden Unexpected Death in Epilepsy in a Mouse Model of Dravet Syndrome Thesis 2023-01-01
• Faust TE, Xin W, Lee B et al. Astrocyte redox dysregulation causes prefrontal hypoactivity: sulforaphane treats non-ictal pathophysiology in ALDH7A1-mediated epilepsy bioRxiv 2019-10-07 (IF/IHC, Mouse)
• Lala S Identification and Characterization of Engulfing CD38+ Periarterial Brain Border Macrophages Thesis Jul 13 1905
• Pisanu A, Lecca D, Mulas G et al. Dynamic changes in pro- and anti-inflammatory cytokines in microglia after PPAR-gamma agonist neuroprotective treatment in the MPTPp mouse model of progressive Parkinson's disease Neurobiol. Dis. 2014-08-15 [PMID: 25134730]
• Jiang Ying, Li Longling, Liu Bin et al. Vagus nerve stimulation attenuates cerebral ischemia and reperfusion injury via endogenous cholinergic pathway in Rat. PloS One 2014-01-01 [PMID: 25036185] (ICC/IF, Rat)
• Kanyshkova T, Ehling P, Cerina M et al. Regionally specific expression of high-voltage-activated calcium channels in thalamic nuclei of epileptic and non-epileptic rats Mol. Cell. Neurosci. 2014-06-07 [PMID: 24914823] (IHC-FrFl, Rat)
  
  Details:
  Iba1 antibody used for IHC-free-floating brains sections from WAG/Rij and ACI rats - transcardially perfused with PBS- ice-cold 4% PFA 35–40 min, brains removed, postfixed for 4 h in 4% PFA/PBS, cryoprotected in 25% sucrose, cut coronal sections 40 um at the level of lateral geniculate nucleus /dLGN, slices collected/washed in TBS, blocked with 10% normal horse serum/2% BSA in 0.3% Triton X-100 -TBS for 2 h, incubated with primary 4 C /16-18 h - 1:100 dilution followed by Cy2-conjugated donkey anti-goat IgG. (Figure 4C, used as microglia marker)
• L'episcopo F, Tirolo C, Testa N, Caniglia S, Morale MC, Deleidi M, Serapide MF, Pluchino S, Marchetti B. Plasticity of Subventricular Zone Neuroprogenitors in MPTP (1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine) Mouse Model of Parkinson's Disease Involves Cross Talk between Inflammatory and Wnt/?-Catenin Signaling Pathways: Functional Consequences for Neuroprotection and Repair. J Neurosci;32(6):2062-2085. 2012-02-08 [PMID: 22323720]
• Wang Y, Dang Z, Wang X et al. Obacunone Alleviates Chronic Pelvic Pain and Pro-Inflammatory Depolarization of Macrophage Induced by Experimental Autoimmune Prostatitis in Mice Available at SSRN 2023-01-01 (Immunohistochemistry, Mouse)
• Lin PBC The Role of INPP5D in Microglial Function and Amyloid Pathogenesis Thesis 2023-01-01
• Ho ES Local delivery of PACAP attenuates inflammation and promotes functional recovery in a mouse model of stroke injury Thesis 2023-01-01
• Ghaffari D, Griffin J, St George-Hyslop P ABI3 deletion in TgCRND8 mice is associated with reduced amyloid plaque pathology and altered glial response bioRxiv 2023-10-09
• Hagen K, Gordon P, Frederick A et al. CRYAB plays a role in terminating the presence of pro-inflammatory macrophages in the older, injured mouse peripheral nervous system Neurobiology of Aging 2024-01-01 (IHC, Mouse)
• Sahragard F Safety assessment of a novel implantable optical sensor designed to monitor spinal cord hemodynamics and oxygenation Thesis 2023-01-01 (ICC/IF, Porcine)
• Morton L, Arndt P, Garza A et al. Spatio-temporal dynamics of microglia phenotype in human and murine cSVD: impact of acute and chronic hypertensive states bioRxiv 2023-08-17 (IHC)
• L'episcopo F, Tirolo C, Testa N et al. Aging-Induced Nrf2-ARE Pathway Disruption in the Subventricular Zone Drives Neurogenic Impairment in Parkinsonian Mice via PI3K-Wnt/beta-Catenin Dysregulation. J Neurosci 2013-01-23 [PMID: 23345222] (IHC-Fr, ICC/IF, Mouse)
• Groh J, Abdelwahab T, Kattimani Y et al. Genetically perturbed myelin as a risk factor for neuroinflammation-driven axon degeneration bioRxiv 2023-07-19 (IHC, Mouse)
• Murage B Characterisation of a Model of ALS8 in the Rat Thesis 2023-01-01 (Immunocytochemistry/ Immunofluorescence)
• Stridh L, Smith PL, Naylor AS et al. Regulation of toll-like receptor 1 and -2 in neonatal mice brains after hypoxia-ischemia. J Neuroinflammation. 2011-05-10 [PMID: 21569241] (IHC-P, Mouse)
• Bheemanapally K A Study of Ventromedial Nucleus of Hypothalamus Glucoregulatory Transmitter Expression and Glucogenic Energy Substrates in Rat Thesis 2022-01-01
• Pan R, Zhang J, Wang J et al. Intermittent fasting protects against Alzheimer�s disease in mice by altering metabolism through remodeling of the gut microbiota Nature Aging 2022-11-17 (Immunohistochemistry, Mouse)
• McKinsey GL, Lizama CO, Keown-Lang AE, Niu A New Tools for Genetically Targeting Myeloid Populations in the Central Nervous System bioRxiv 2019-12-23 (IF/IHC, Mouse)
• Kanyshkova T, Broicher T, Meuth SG, Pape HC, Budde T. A-type K+ currents in intralaminar thalamocortical relay neurons. Pflugers Arch;461(5):545-56. 2011-05-01 [PMID: 21437601] (ICC/IF, Rat)
• MacIsaac S, Quevedo Melo T, Zhang Y et al. Neuron-autonomous susceptibility to induced synuclein aggregation is exacerbated by endogenous Lrrk2 mutations and ameliorated by Lrrk2 genetic knock-out Brain Communications Jan 1 2020 (ICC/IF, Mouse)
• Jurga M, Dainiak MB, Sarnowska A et al. The performance of laminin-containing cryogel scaffolds in neural tissue regeneration. Biomaterials 2011-05-01 [PMID: 21324403] (IHC-F, Rat)
• Jegou JF, Chan P, Schouft MT, Griffiths MR, Neal JW, Gasque P, Vaudry H, Fontaine M. C3d binding to the myelin oligodendrocyte glycoprotein results in an exacerbated experimental autoimmune encephalomyelitis. J Immunol;178(5):3323-31. 2007-03-01 [PMID: 17312184]
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• Xia D, Lianoglou S, Sandmann T, et al. Fibrillar A beta causes profound microglial metabolic perturbations in a novel APP knock-in mouse model bioRxiv 2021-01-20 (ICC/IF, Mouse)
• Kalm M, Lannering B, Bjork-Eriksson T, Blomgren K. Irradiation-induced loss of microglia in the young brain. J Neuroimmunol;206(1-2):70-5. 2009-01-03 [PMID: 19070908]
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Reviews for AIF-1/Iba1 Antibody (NB100-1028) (26)

Average Rating: 4.5

(Based on 26 reviews)

We have 26 reviews tested in 7 species: Human, Mouse, Rat, Canine, Feline, Horse, Human and Mouse.

reviewed by: Verified Customer IHC-P Human 04/24/2024

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Tonsil
• Species: Human
• Lot: S7C7G2P56

Comments

• Comments: Section underwent heat induced epitope retrieval in a vegetable steamer for 20m using Target Retrieval Solution.

reviewed by: Verified Customer IHC-P Horse 04/24/2024

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Spleen
• Species: Horse
• Lot: S7C7G2P56

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.*** Section underwent heat induced epitope retrieval in a vegetable steamer for 20m using Target Retrieval Solution.

reviewed by: Verified Customer IHC-P Mouse 04/24/2024

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Spleen
• Species: Mouse
• Lot: S7C7G2P56

Comments

• Comments: Section underwent heat induced epitope retrieval in a vegetable steamer for 20m using Target Retrieval Solution.

reviewed by: Verified Customer IHC-Fr Mouse 04/27/2023

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Brain
• Species: Mouse
• Lot: S7C7G2P44

reviewed by: Deepak Gupta IHC-Fr Mouse 04/25/2023

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: 20 um Mouse brain Tissue
• Species: Mouse
• Lot: 87C7G2P41

reviewed by: Verified Customer IHC-P Canine 10/29/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Brain
• Species: Canine
• Lot: S7C7G2P39

reviewed by: Verified Customer IHC-P Canine 07/03/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Liver
• Species: Canine
• Lot: S7C7G2P41

Comments

• Comments: Following deparaffinization, pre-heated >=90C Target Retrieval Solution - Dako S1699 - was added to slides and then the slide container was placed into a vegetable steamer for 20min. Following antigen retrieval, slides were cooled for 15min, washed with 1X PBS, then placed in 3 percent hydrogen peroxide diluted in methanol. Following peroxide quench, slides were sequentially incubated - 10min each at room temperature with washes in between - in avidin and biotin solution -abcam ab64212- then Dako Serum-Free Protein Block - Dako X0909. After incubation with the protein block, slides were incubated with the primary antibody diluted 1:500 in Dako Antibody Diluent - Dako S3022 - for 30 min at room temperature. Slides were then incubated with rabbit anti-goat - Vector Labs BA5000 - diluted at 1:200 in Dako Serum-Free Protein Block - Dako X0909. Slides were then incubated with VECTASTAIN Elite ABC-HRP Reagent - Vector Labs PK-7100 - for 30min at room temperature. Staining was then developed using Dako DAB as a chromogen.

reviewed by: Verified Customer IHC-P Mouse 08/12/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Adult mouse brain tissue
• Species: Mouse
• Lot: S7c7G2p41

Comments

• Comments: 1/100 dilution Overnight in PBS at 4*C

reviewed by: Lucia Lisi IHC-P Human 01/17/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Brain Tissue Sections,Brain tissue,Human Brain Tissue
• Species: Human
• Lot: S9C3G2

reviewed by: Emily Thompson Immunohistochemistry-PFA fixed, free floating Mouse 12/14/2019

Summary

• Application: Other
• Sample Tested: PFA fixed mouse brain,SCID mouse cortex injected with human glioblastoma cells
• Species: Mouse
• Lot: S7C7G2P13

Comments

• Comments: 1:250

reviewed by: Verified Customer ICC Mouse 11/21/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: adult mouse neural stem cells
• Species: Mouse
• Lot: 87C7G2P26

reviewed by: J. Sebastián Jara Whole-mount tissue immunolabeling Mouse 08/12/2019

Summary

• Application: Other
• Sample Tested: Dorsal root ganglion
• Species: Mouse
• Lot: S7C7G2P34E161018

Comments

• Comments: Good signal to noise ratio for 3D imaging. ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

reviewed by: Verified Customer IHC-Fr Feline 07/15/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: optic nerve
• Species: Feline
• Lot: S9G2P3E240918

reviewed by: Verified Customer IHC-Fr Mouse 05/15/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse brain
• Species: Mouse
• Lot: SC7G2P23

reviewed by: Kevin Thorburn IHC-Fr Mouse 11/29/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: mouse spinal cord
• Species: Mouse
• Lot: S7G7G2P19

reviewed by: Verified Customer IHC-P Canine 08/07/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Optic nerve
• Species: Canine
• Lot: S7C7G2P15

reviewed by: Verified Customer IF Mouse 04/23/2018

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse brain
• Species: Mouse
• Lot: S7G7G2P19

reviewed by: Fuyi Chen immunohistochemistry free floating tissue Human and Mouse 03/16/2018

Summary

• Application: Other
• Sample Tested: Mouse brain
• Species: Human and Mouse
• Lot: S7G7G2P15E031117

reviewed by: Haiyan Qiu ICC Mouse 04/01/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: brain and spinal cord
• Species: Mouse

reviewed by: Verified Customer IHC-Fr Mouse 09/22/2016

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Spinal cord
• Species: Mouse

reviewed by: Verified Customer IF Mouse 05/11/2016

Summary

• Application: Immunofluorescence
• Sample Tested: mouse spinal cord
• Species: Mouse
• Lot: S7C7P6

reviewed by: Verified Customer IHC-Fr Rat 10/08/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Rat frozen 4% paraformalehyde fixed brain sections
• Species: Rat

reviewed by: Xin Luo IHC-P Mouse 06/30/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: mouse brain
• Species: Mouse

reviewed by: Jaycie Loewen IF Mouse 07/22/2013

Summary

• Application: Immunofluorescence
• Species: Mouse

reviewed by: Grace zhou IF Rat 07/16/2012

Summary

• Application: Immunofluorescence
• Sample Tested: brain and spinal cord
• Species: Rat
• Lot: s5c2g2

reviewed by: Verified Customer IF 06/26/2012

Summary

• Application: Immunofluorescence

FAQs for AIF-1/Iba1 Antibody (NB100-1028). (Showing 1 - 2 of 2 FAQs).

1. What is the volume of the sample size (NB100-1028SS)?  ..show answer..
   • According to our datasheet, NB100-1028SS is a package containing 0.02 mg, with the concentration of 0.5 mg/ml.  Therefore, the vial should contain 40 ul.
2. The experimental method in the NB100-1028 instruction is not clear. We use frozen sections of brain tissue. But we can't produce satisfactory results. Could you provide us with a set of experimental methods in detail to help us get the results of the experiment?  ..show answer..
   • With regards to this AIF-1/Iba1 Antibody NB100-1028, we unfortunately do not have any suggested protocol for this product. If you would like, you can please refer to our general protocol

Bioinformatics

• Gene Symbol: AIF1
• Entrez:
  • 199(Human)
  • 11629(Mouse)
  • 29427(Rat)
• Uniprot:
  • NP_001614.3(Human)
  • NP_116573.1(Human)