AIF Antibody

Western Blot: AIF Antibody [NBP1-52446] - (A), mouse (B), and rat (C) liver tissue lysates

Immunocytochemistry: AIF Antibody [NBP1-52446] - K562 cells.

Immunohistochemistry-Paraffin: AIF Antibody [NBP1-52446] - Analysis of anti-AIFM1 / AIF antibody with human kidney at concentration 5 ug/ml.

Western Blot: AIF Antibody [NBP1-52446] - Western blot analysis of AIF in K562 with AIF antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.

Immunohistochemistry-Paraffin: AIF Antibody [NBP1-52446] - Staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Discontinued

AIF Antibody Summary

• Immunogen: A 14 amino acid peptide near the C-terminus of human AIF. The immunogen is located within the last 50 amino acids of AIF.
• Predicted Species: Xenopus (93%), Feline (100%), Opossum (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AIFM1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.25-1 ug/ml

Reactivity Notes

Gorilla, Gibbon, Marmoset. Predicted cross-reactivity based on sequence identity: Turkey (86%), Chicken (86%), Zebrafish (86%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for AIF Antibody

• AIF
• AIFapoptosis-inducing factor 1, mitochondrial
• AIFM1
• apoptosis-inducing factor, mitochondrion-associated, 1
• COXPD6
• MGC111425
• PDCD8
• PDCD8striatal apoptosis-inducing factor
• programmed cell death 8 (apoptosis-inducing factor)
• Programmed cell death protein 8

Background

AIF, or apoptosis-inducing factor, is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death, or apoptosis (1,2). AIF translocates to the nucleus when apoptosis is induced and causes mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. Apoptotic activity of AIF leads to chromatin condensation and DNA fragmentation (1). Similar to cytochrome c, AIF is a bifunctional protein with an oxidoreductase function and a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion (2,3,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AIFM1
• Entrez:
  • 51060(Human)
• Uniprot:
  • O95831(Human)