AKAP95/AKAP8 Antibody (3D4) - Azide and BSA Free

Western Blot: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - AKAP8 monoclonal antibody (M01), clone 3D4 Analysis of AKAP8 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - Analysis of monoclonal antibody to AKAP8 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - Analysis of monoclonal antibody to AKAP8 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.

Western Blot: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - Analysis of AKAP8 expression in transfected 293T cell line by AKAP8 monoclonal antibody (M01), clone 3D4.Lane 1: AKAP8 transfected lysate(76.2 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - Analysis of AKAP8 transfected lysate using anti-AKAP8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AKAP8 monoclonal antibody.

Sandwich ELISA: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - Detection limit for recombinant GST tagged AKAP8 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 3D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

AKAP95/AKAP8 Antibody (3D4) - Azide and BSA Free Summary

• Immunogen: AKAP8 (NP_005849, 551 a.a. ~ 662 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAES
• Marker: Nucleus without Nucleoli Marker
• Specificity: AKAP8 - A kinase (PRKA) anchor protein 8
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AKAP8
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AKAP95/AKAP8 Antibody (3D4) - Azide and BSA Free

• A kinase (PRKA) anchor protein 8
• AKAP-8
• AKAP-95 AKAP 95
• A-kinase anchor protein 8
• A-kinase anchor protein 95 kDa
• A-kinase anchor protein, 95kDa
• DKFZp586B1222

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AKAP8
• Entrez:
  • 10270(Human)
• OMIM:
  • 604692(Human)
• Uniprot:
  • NP_005849(Human)