AKAP95/AKAP8 Antibody

Western Blot: AKAP95/AKAP8 Antibody [NBP1-79139] - Western blot analysis of AKAP95/AKAP8 expression in 1) A431 and 2) NIH-3T3 whole cell lysates.

Immunocytochemistry/Immunofluorescence: AKAP95/AKAP8 Antibody [NBP1-79139] - AKAP95/AKAP8 antibody was tested in HeLa cells at 1:500 with DyLight 488 (green). Nuclei and alpha Tubulin (NB100-690) were counterstained with DAPI (blue) and DyLight 550 (red).

Immunohistochemistry: AKAP95/AKAP8 Antibody [NBP1-79139] - IHC staining of AKAP95/AKAP8 in human ovarian cancer using DAB with hematoxylin counterstain.

Product Discontinued

AKAP95/AKAP8 Antibody Summary

• Immunogen: Human AKAP95/AKAP8 peptide within amino acids 650 to 692 [Swiss-Prot# O43823].
• Localization: Nucleus matrix. Note: Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis.
• Marker: Nucleus without Nucleoli Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AKAP8
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000
• Application Notes: This AKAP95/AKAP8 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western Blot, and Immunohistochemistry on paraffin-embedded sections. Use in Flow Cytometry reported in scientific literature (PMID: 16980585). In ICC/IF, nuclear staining was observed in HeLa cells. In Western Blot, a band can be seen at ~95 kDa representing AKAP95/AKAP8. In IHC-P, staining was observed in the nucleus, specifically nucleoli of human ovarian cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for AKAP95/AKAP8 Antibody

• A kinase (PRKA) anchor protein 8
• AKAP-8
• AKAP-95 AKAP 95
• A-kinase anchor protein 8
• A-kinase anchor protein 95 kDa
• A-kinase anchor protein, 95kDa
• DKFZp586B1222

Background

AKAP95 (A-kinase anchor protein 95 kD; murine gene - Akap8) is an anchoring protein which is responsible for the subcellular compartmentalization of PKA type II (cAMP-dependent protein kinase). AKAP95 localizes to nuclear matrix along with fidgetin and gets redistributed or detached from condensed chromatin during mitotic cell division. AKAP95 is expressed in heart, liver, skeletal muscle, kidney, pancreas etc. and shows a broad expression pattern overlapping with that of fidgetin during murine embryogenesis. AKAP95 can interact with fidgetin and binds to several proteins such as regulatory subunit of type II cAMP-dependent protein kinase (during mitosis), Eg7 of chromosome-condensing complex, p68 RNA helicase, c-Myc-binding protein, minichromosome maintenance 2 protein, and D-type cyclins as well as phosphodiesterases for serving diverse cellular functions including PKA signaling, DNA condensation, transcription, and DNA replication. Moreover, AKAP95 has recently been shown to implicate in cAMP-regulated mRNA stability as well as the nuclear translocation of active caspase 3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AKAP95/AKAP8 Antibody (NBP1-79139)(1)

Publication using NBP1-79139

• Li Y, Kao GD, Garcia BA, Shabanowitz J, Hunt DF, Qin J, Phelan C, Lazar MA. A novel histone deacetylase pathway regulates mitosis by modulating Aurora B kinase activity. Genes Dev. 20(18):2566-79. PubMed PMID: 16980585. 2006-09-15 [PMID: 16980585] (ICC/IF, FLOW, IP, Human)

Bioinformatics

• Gene Symbol: AKAP8
• Entrez:
  • 10270(Human)
• Uniprot:
  • O43823(Human)