Albumin Antibody - BSA Free

ELISA: Albumin Antibody [NB300-336] - Direct ELISA Test. Human serum albumin protein as test antigen. Affi-pure anti-HSA IgY as primary antibody (0.1 ug/ml), and Rabbit anti-IgY-HRP as 2nd antibody. ( anti-ALB )

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Albumin Antibody - BSA Free Summary

• Immunogen: Recombinant serum albumin (GI # 113576) sequence 1-609
• Localization: Secreted
• Specificity: Albumin
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: ALB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:200-1:500
• Application Notes: This antibody can be used in ELISA and Western Blot. In Western Blot a 69.4 kDa band is seen. Recommended Starting Dilutions*: ELISA: 0.1-0.5 ug/ml WB: 1/1000 Not tested in other applications. *Optimal dilutions should be determined by the end user.
• Control:

  Human Serum Albumin Overexpression Lysate

Reactivity Notes

Cross-reacts with Human, Mouse, Rat, Cow, Dog and Pig. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for Albumin Antibody - BSA Free

• ALB
• Albumin
• cell growth inhibiting protein 42
• DKFZp779N1935
• growth-inhibiting protein 20
• PRO0883
• PRO0903
• PRO1341
• serum albumin

Background

Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALB
• Entrez:
  • 213(Human)
  • 11657(Mouse)
  • 24186(Rat)