Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Western Blot: ALDH1A1 Antibody [31160002] - Western blot analysis of ALDH1A1 in HepG2 whole cell lysates.

Immunocytochemistry/Immunofluorescence: ALDH1A1 Antibody [31160002] - Detection of ALDH1A1 (Green) in HepG2 cells using 31160002. Nuclei (Blue) are counterstained using Hoechst 33258.

Immunohistochemistry: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [31160002] - Immunohistochemical staining of ALDH1A1 in tumors derived from CL16 cells transduced with either miR-155 or Ctrl vector. Expression of the three targets in non-metastatic NM-2C5 (high miR-155) and metastatic CL16 cells (low miR-155) grown in vitro was also evaluated. Objective 40x, Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (oncotarget.com/article/4942/text/), licensed under a CC-BY license

ELISA: ALDH1A1 Antibody [31160002]

Product Discontinued

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody Summary

• Immunogen: This ALDH1A1 antibody is specific for the N Terminus Region of the human ALDH1A1 protein.
• Marker: Astrocyte Marker
• Specificity: This product is specific for Human ALDH1A1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALDH1A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:5000
• Application Notes: This antibody is useful in ELISA, Immunofluorescence, Immunohistochemistry-Paraffin and Western Blot.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

• acetaldehyde dehydrogenase 1
• ALDC
• aldehyde dehydrogenase 1 family, member A1
• aldehyde dehydrogenase 1, soluble
• Aldehyde Dehydrogenase 1A1
• Aldehyde Dehydrogenase 1-A1
• Aldehyde dehydrogenase family 1 member A1
• Aldehyde dehydrogenase, cytosolic
• aldehyde dehydrogenase, liver cytosolic
• ALDH class 1
• ALDH1
• ALDH11
• ALDH1A1
• ALDH-E1
• ALHDII
• EC 1.2.1
• EC 1.2.1.36
• MGC2318
• PUMB1
• RALDH 1
• RALDH1
• retinal dehydrogenase 1
• retinaldehyde dehydrogenase 1

Background

Usual human livers contain two major ALDH isozymes, i.e., cytosolic ALDH1 and mitochondrial ALDH2(1). Human red cell aldehyde dehydrogenase (ALDH) resembles the liver cytosolic isozyme in numerous physicochemical properties. Thus, the erythrocyte enzyme, by virtue of its chemical and structural identity with the liver cytosolic enzyme, may serve as a suitable peripheral enzyme model to understand the cause and mechanism of alcohol abuse-related changes in liver cytosolic ALDH that has been found to be reduced in alcoholics (2). ALDH1A1 is known to catalyze the oxidation of retinaldehyde to retinoic acid. Overexpression of human ALDH1A1 in HEK cells led to a more than 20-fold increase in 3-deoxyglucosone dehydrogenase activity (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (31160002)(3)

Publications using 31160002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Thomsen KG, Terp MG, Lund RR et al. miR-155, identified as anti-metastatic by global miRNA profiling of a metastasis model, inhibits cancer cell extravasation and colonization in vivo and causes significant signaling alterations. Oncotarget 2015-10-06 [PMID: 26317550] (IF/IHC)
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

FAQs for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (31160002). (Showing 1 - 1 of 1 FAQs).

1. I am looking for an antibody for ALDH that does not cross react with mouse.
   • The ALDH1A1 sequences between human and mouse are very similar. The major differences in sequence are at the N-terminal and 31160002 uses an immunogen at the N-terminus. This antibody hasn't been tested to not work in mouse, and we can not guarantee that it doesn't recognize the mouse protein, but this is the antibody that has the best chance of only reacting in humans that we carry.

Bioinformatics

• Gene Symbol: ALDH1A1
• Entrez:
  • 216(Human)
• Uniprot:
  • P00352()