Alkaline Phosphatase/ALPL Antibody (B4-78) [Allophycocyanin]

BG01V human embryonic stem cells were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL APC-conjugated Monoclonal Antibody (Catalog # FAB1448A, filled histogram) or isotype control antibody (IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

iPSCs were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL APC-conjugated Monoclonal Antibody (Catalog # FAB1448A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: B4-78
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Alkaline Phosphatase/ALPL Antibody (B4-78) [Allophycocyanin] Summary

• Immunogen: Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL
• Specificity: Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALPL
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Alkaline Phosphatase/ALPL Antibody (B4-78) [Allophycocyanin]

• Akp2
• Alkaline phosphatase liver/bone/kidney isozyme
• alkaline phosphatase, liver/bone/kidney
• alkaline phosphatase, tissue-nonspecific isozyme
• alkaline phosphomonoesterase
• ALPL
• APTNAP
• AP-TNAP
• EC 3.1.3.1
• FLJ40094
• FLJ93059
• glycerophosphatase
• HOPS
• liver/bone/kidney-type alkaline phosphatase
• MGC161443
• tissue-nonspecific ALP
• TNAP
• TNSALP
• TNSALPMGC167935

Background

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a Glycosyl Phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Alkaline Phosphatase/ALPL Antibody (FAB1448A)(14)

Publications using FAB1448A

• Ying Liu, Yan Chen, Xiao-Hua Li, Chong Cao, Hui-Xi Zhang, Cui Zhou et al. Dissection of Cellular Communication between Human Primary Osteoblasts and Bone Marrow Mesenchymal Stem Cells in Osteoarthritis at Single-Cell Resolution International Journal of Stem Cells 2023-08-30 [PMID: 37105556]
• Saula Vigili de Kreutzenberg, Alessandra Giannella, Giulio Ceolotto, Elisabetta Faggin, Roberta Cappellari, Marta Mazzucato et al. A miR-125/Sirtuin-7 pathway drives the pro-calcific potential of myeloid cells in diabetic vascular disease Diabetologia 2022-09-01 [PMID: 35708762]
• Chirayu Patel, Lihong Shi, John F. Whitesides, Brittni M. Foster, Roberto J. Fajardo, Ellen E. Quillen et al. A New Method of Bone Stromal Cell Characterization by Flow Cytometry Current Protocols 2022-03-29 [PMID: 35349226]
• T Sauer, G Facchinett, M Kohl, JM Kowal, S Rozanova, J Horn, H Schmal, I Kwee, AP Schulz, S Hartwig, M Kassem, JK Habermann, T Gemoll Protein Expression of AEBP1, MCM4, and FABP4 Differentiate Osteogenic, Adipogenic, and Mesenchymal Stromal Stem Cells International Journal of Molecular Sciences, 2022-02-25;23(5):. 2022-02-25 [PMID: 35269711] (Flow Cytometry, Human)
• Yun Gong, Junxiao Yang, Xiaohua Li, Cui Zhou, Yu Chen, Zun Wang et al. A systematic dissection of human primary osteoblasts in vivo at single-cell resolution Aging (Albany NY) 2021-08-31 [PMID: 34428745]
• Justyna Magdalena Kowal, Sören Möller, Dalia Ali, Florence Figeac, Torben Barington, Hagen Schmal et al. Identification of a clinical signature predictive of differentiation fate of human bone marrow stromal cells Stem Cell Research & Therapy 2021-12-01 [PMID: 33941262]
• Justyna M. Kowal, Hagen Schmal, Ulrich Halekoh, Jacob B. Hjelmborg, Moustapha Kassem Single-cell high-content imaging parameters predict functional phenotype of cultured human bone marrow stromal stem cells Stem Cells Translational Medicine 2020-02-01 [PMID: 31758755]
• F Sassi, I Buondonno, C Luppi, E Spertino, E Stratta, M Di Stefano, M Ravazzoli, G Isaia, M Trento, P Passera, M Porta, GC Isaia, P D'Amelio Type 2 diabetes affects bone cells precursors and bone turnover BMC Endocr Disord, 2018-08-08;18(1):55. 2018-08-08 [PMID: 30089481] (Flow Cytometry, Human)
• A Di Nisio, L De Toni, MS Rocca, M Ghezzi, R Selice, G Taglialavo, A Ferlin, C Foresta Negative Association Between Sclerostin and INSL3 in Isolated Human Osteocytes and in Klinefelter Syndrome: New Hints for Testis-Bone Crosstalk J. Clin. Endocrinol. Metab., 2018-05-01;0(0):. 2018-05-01 [PMID: 29452406] (Flow Cytometry, Human)
• Y Nagata, T Kiyono, K Okamura, YI Goto, M Matsuo, M Ikemoto-Ue, N Hashimoto Interleukin-1beta (IL-1?)-induced Notch ligand Jagged1 suppresses mitogenic action of IL-1? on human dystrophic myogenic cells PLoS ONE, 2017-12-01;12(12):e0188821. 2017-12-01 [PMID: 29194448] (Flow Cytometry, Human)
• Julia Collin, Mario Gössl, Yoshiki Matsuo, Rebecca R. Cilluffo, Andreas J. Flammer, Darrell Loeffler et al. Osteogenic monocytes within the coronary circulation and their association with plaque vulnerability in patients with early atherosclerosis International Journal of Cardiology 2015-02-15 [PMID: 25482280] (Flow Cytometry, Human)
• The C-terminus of tissue factor pathway inhibitor alpha is required for its interaction with factors V and Va. J. Thromb. Haemost., 2012-09-01;10(9):1944-6. 2012-09-01 [PMID: 22738072] (Flow Cytometry, Human)
• Michael D. Ball, Ian C. Bonzani, Melissa J. Bovis, Andrew Williams, Molly M. Stevens Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study Clinical Orthopaedics & Related Research 2011-11-01 [PMID: 21547415]
• Klim JR, Li L, Wrighton PJ A defined glycosaminoglycan-binding substratum for human pluripotent stem cells. Nat. Methods, 2010-11-14;7(12):989-94. 2010-11-14 [PMID: 21076418] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ALPL