Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193] - Analysis of Human engineered ALPL cells using Alkaline Phosphatase. Primary antibody dilution: 1:1000. Image from verified customer review.

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis of ovarian cancer tissues using Alkaline Phosphatase, Tissue Non-Specific mouse mAb with DAB staining.

Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193] - Analysis of Human engineered ALPL cells using Alkaline Phosphatase. Image from verified customer reviews.

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis using Alkaline Phosphatase, Tissue Non-Specific mAb against human Alkaline Phosphatase, Tissue Non-Specific (AA: 18-502) recombinant protein. (Expected MW is 78.9 kDa)

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis using Alkaline Phosphatase, Tissue Non-Specific mouse mAb against HeLa (1), and NTERA-2 (4) cell lysate.

Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis of MCF-7 cells using Alkaline Phosphatase, Tissue Non-Specific mouse mAb (green) and negative control (red).

ELISA: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, CyTOF-ready
• Clone: 2F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Alkaline Phosphatase, Tissue Non-Specific expressed in E. Coli.
• Localization: Cytoplasm
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALPL
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 57.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free

• Alkaline phosphatase liver/bone/kidney isozyme
• alkaline phosphatase, liver/bone/kidney
• alkaline phosphatase, tissue-nonspecific isozyme
• alkaline phosphomonoesterase
• APTNAP
• AP-TNAP
• EC 3.1.3.1
• FLJ40094
• FLJ93059
• glycerophosphatase
• HOPS
• liver/bone/kidney-type alkaline phosphatase
• MGC161443
• tissue-nonspecific ALP
• TNAP
• TNSALPMGC167935

Background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Alkaline Phosphatase, Tissue Non-Specific Antibody (NBP2-22193)(2)

Publications using NBP2-22193

• Kamadjaja DB, Satriyo H, Setyawan A Et al. Analyses of Bone Regeneration Capacity of Freeze-Dried Bovine Bone and Combined Deproteinized-Demineralized Bovine Bone Particles in Mandibular Defects: The Potential Application of Biological Forms of Bovine-Bone Filler European journal of dentistry 2021-11-23 [PMID: 34814221] (IF/IHC, Rabbit)
• Raimondi L, De Luca A, Gallo A et al. Osteosarcoma cell-derived exosomes affect tumor microenvironment by specific packaging of microRNAs Carcinogenesis 2019-07-10 [PMID: 31294446]

Reviews for Alkaline Phosphatase, Tissue Non-Specific Antibody (NBP2-22193) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Ming Lu WB Human 09/13/2022

Summary

• Application: Western Blot
• Sample Tested: engineered ALPL cells
• Species: Human
• Lot: 140226

reviewed by: Ming Lu Flow Human 09/13/2022

Summary

• Application: Flow Cytometry
• Sample Tested: engineered ALPL cells
• Species: Human
• Lot: 140226

Bioinformatics

• Gene Symbol: ALPL