alpha-Internexin Antibody (2E4)

Product Discontinued

alpha-Internexin Antibody (2E4) Summary

• Immunogen: Recombinant rat alpha-internexin/NF66
• Marker: Immature Neuronal Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: INA
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:2000-1:5000
  • Immunohistochemistry-Frozen 1:2000-1:5000
  • Immunohistochemistry-Paraffin 1:2000-1:5000
  • Western Blot 1:500-1:2500
• Application Notes: For immunohistochemistry, the antibody can be used on formalin-fixed, paraffin-embeded tissue sections, formalin-fixed tissue cultured cells, and on frozen tissue sections. The epitope recognized by the 2E3 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation. Hence, the 2E3 antibody is well suited for studies of paraffin-embeded, formalin-fixed tissue sections. On Western blots, alpha-internexin is detected as a major band of ~64-66 kDa (product citation 3). Minor bands at ~150 kDa may represent dimers, and bands at ~50 kDa represent alpha-internexin breakdown products. The 1D2 antibody has been evaluated in human and rat, and based on sequence homology is exprected to cross-react with all mammals.
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Rat. Predicted to React with most Mammals based on sequence homology.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 1 mg/ml BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for alpha-Internexin Antibody (2E4)

• 66 kDa neurofilament protein
• alphaInternexin
• alpha-Internexin
• alpha-Inx
• FLJ18662
• FLJ57501
• INA
• internexin neuronal intermediate filament protein, alpha
• MGC12702
• NEF5
• neurofilament 5 (66kD)
• Neurofilament 5
• Neurofilament-66
• neurofilament-66, tax-binding protein
• NF-66
• NF-66alpha-internexin
• TXBP-1

Background

Alpha-internexin is a Class IV intermediate filament, related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development procedes. However, many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H, and some mature neurons only express the alpha-internexin subunit of neurofilament.

The very early developmental expression of alpha-internexin suggests that its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. Therefore, alpha-internexin antibodies serve as unique probes to study and classify neuronal types and follow their processes in tissue sections and culture.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INA