Aminopeptidase N/CD13 Antibody [Unconjugated]

Aminopeptidase N/CD13 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Aminopeptidase N/CD13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2335) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Aminopeptidase N/CD13 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse Aminopeptidase N/CD13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2335) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to apical membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Mu
• Applications: WB, Flow, IHC, IP, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Aminopeptidase N/CD13 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Aminopeptidase N/CD13
  Lys69-Ser966
  Accession # P97449
• Specificity: Detects mouse Aminopeptidase N/CD13 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ANPEP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 0.1-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aminopeptidase N/CD13 Antibody [Unconjugated]

• alanyl (membrane) aminopeptidase
• Alanyl aminopeptidase
• Aminopeptidase M
• Aminopeptidase N
• ANPEP
• AP-M
• APN
• AP-N
• CD13 antigen
• CD13
• CD13APN
• EC 3.4.11
• EC 3.4.11.2
• gp150
• LAP1
• Microsomal aminopeptidase
• Myeloid plasma membrane glycoprotein CD13
• p150
• PEPN
• PEPNhAPN

Background

The mouse Anpep gene encodes Aminopeptidase N (APN), which is also known as microsomal aminopeptiase, alanyl aminopeptidase, Aminopeptidase M, CD13, or membrane protein p161 (1‑3). The deduced amino acid sequence of mouse APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (4).

1. Chen, H. et al. (1996) J. Immunol. 157:2593.
2. Larsen, S.L. et al. (1996) J. Exp. Med. 184:183.
3. Hansen, A.S. et al. (1993) Eur. J. Immunol. 23:2358.
4. Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 289 Academic Press, San Diego.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aminopeptidase N/CD13 Antibody (AF2335)(57)

Publications using AF2335

• Weber, RZ;Achon Buil, B;Rentsch, NH;Bosworth, A;Zhang, M;Kisler, K;Tackenberg, C;Zlokovic, BV;Rust, R; A molecular brain atlas reveals cellular shifts during the repair phase of stroke bioRxiv : the preprint server for biology 2024-08-22 [PMID: 39229128] (Immunohistochemistry, Mouse)
• Bennett, HC;Zhang, Q;Wu, YT;Manjila, SB;Chon, U;Shin, D;Vanselow, DJ;Pi, HJ;Drew, PJ;Kim, Y; Aging drives cerebrovascular network remodeling and functional changes in the mouse brain Nature communications 2024-07-30 [PMID: 39080289] (Immunohistochemistry, Transgenic Mouse)
• Dong, J;Wang, L;Sullivan, BT;Sun, L;Chang, L;Martinez Smith, VM;Ding, J;Le, W;Gerfen, CR;Cai, H; Patch and matrix striatonigral neurons differentially regulate locomotion bioRxiv : the preprint server for biology 2024-06-12 [PMID: 38915717] (Immunohistochemistry, Transgenic Mouse)
• Wei, C;Jiang, W;Wang, R;Zhong, H;He, H;Gao, X;Zhong, S;Yu, F;Guo, Q;Zhang, L;Schiffelers, LDJ;Zhou, B;Trepel, M;Schmidt, FI;Luo, M;Shao, F; Brain endothelial GSDMD activation mediates inflammatory BBB breakdown Nature 2024-04-17 [PMID: 38632402] (Immunohistochemistry, Mouse, Transgenic Mouse)
• Zhang, D;Ruan, J;Peng, S;Li, J;Hu, X;Zhang, Y;Zhang, T;Ge, Y;Zhu, Z;Xiao, X;Zhu, Y;Li, X;Li, T;Zhou, L;Gao, Q;Zheng, G;Zhao, B;Li, X;Zhu, Y;Wu, J;Li, W;Zhao, J;Ge, WP;Xu, T;Jia, JM; Synaptic-like transmission between neural axons and arteriolar smooth muscle cells drives cerebral neurovascular coupling Nature neuroscience 2024-01-02 [PMID: 38168932] (Immunohistochemistry, Transgenic Mouse)
• Wilk, CM;Cathomas, F;Török, O;Le Berichel, J;Park, MD;Heaton, GR;Hamon, P;Troncoso, L;Scull, BP;Dangoor, D;Silvin, A;Fleischmann, R;Belabed, M;Lin, H;Taouli, EM;Boettcher, S;Manz, MG;Kofler, JK;Yue, Z;Lira, SA;Ginhoux, F;Crary, JF;McClain, KL;Picarsic, JL;Russo, SJ;Allen, CE;Merad, M; Circulating senescent myeloid cells drive blood brain barrier breakdown and neurodegeneration bioRxiv : the preprint server for biology 2023-10-11 [PMID: 37873371] (IHC, Mouse)
• Jovanovic Macura, I;Zivanovic, A;Perovic, M;Ciric, J;Major, T;Kanazir, S;Ivkovic, S; The Expression of Major Facilitator Superfamily Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is Altered in the Retinas of a 5xFAD Mouse Model of Alzheimer's Disease International journal of molecular sciences 2023-09-14 [PMID: 37762391] (IHC, Mouse)
• Samu N Kurki, Pavel Uvarov, Alexey S Pospelov, Kalevi Trontti, Antje K Hübner, Rakenduvadhana Srinivasan et al. Expression patterns of NKCC1 in neurons and non-neuronal cells during cortico-hippocampal development Cerebral Cortex 2023-05-09 [PMID: 36573432]
• Rebecca Z. Weber, Geertje Mulders, Patrick Perron, Christian Tackenberg, Ruslan Rust Molecular and anatomical roadmap of stroke pathology in immunodeficient mice Frontiers in Immunology 2022-12-09 [PMID: 36569903] (Immunohistochemistry, Mouse)
• A Drieu, S Du, SE Storck, J Rustenhove, Z Papadopoul, T Dykstra, F Zhong, K Kim, S Blackburn, T Mamuladze, O Harari, CM Karch, RJ Bateman, R Perrin, M Farlow, J Chhatwal, Dominantly, S Hu, GJ Randolph, I Smirnov, J Kipnis Parenchymal border macrophages regulate the flow dynamics of the cerebrospinal fluid Nature, 2022-11-09;611(7936):585-593. 2022-11-09 [PMID: 36352225] (IHC, Mouse)
• MD Lauver, G Jin, KN Ayers, SN Carey, CS Specht, CS Abendroth, AE Lukacher T cell deficiency precipitates antibody evasion and emergence of neurovirulent polyomavirus Elife, 2022-11-07;11(0):. 2022-11-07 [PMID: 36341713] (IHC, Mouse)
• O'Shea TM, Ao Y, Wang S et al. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice Nature Communications 2022-09-28 [PMID: 36171203]
• Giuseppe Barisano, Kassandra Kisler, Brent Wilkinson, Angeliki Maria Nikolakopoulou, Abhay P. Sagare, Yaoming Wang et al. A “multi-omics” analysis of blood–brain barrier and synaptic dysfunction in APOE4 mice Journal of Experimental Medicine 2022-11-07 [PMID: 36040482] (Immunohistochemistry, Transgenic Mouse)
• Rachel Britton, Angela T. Liu, Sanket V. Rege, Julia M. Adams, Lily Akrapongpisak, David Le et al. Molecular and histological correlates of cognitive decline across age in male C57BL/6J mice Brain and Behavior 2022-09-01 [PMID: 35971662]
• Masamitsu Takashima, Kuniyuki Nakamura, Takuya Kiyohara, Yoshinobu Wakisaka, Masaoki Hidaka, Hayato Takaki et al. Low-dose sodium-glucose cotransporter 2 inhibitor ameliorates ischemic brain injury in mice through pericyte protection without glucose-lowering effects Communications Biology 2022-07-02 [PMID: 35780235]
• S Kiani Shab, S Morabito, EP Danhash, A McQuade, JR Sanchez, E Miyoshi, JP Chadarevia, C Claes, MA Coburn, J Hasselmann, J Hidalgo, KN Tran, AC Martini, W Chang Roth, J Pascual, E Head, DA Hume, C Pridans, H Davtyan, V Swarup, M Blurton-Jo Absence of microglia promotes diverse pathologies and early lethality in Alzheimer's disease mice Cell Reports, 2022-06-14;39(11):110961. 2022-06-14 [PMID: 35705056] (IHC, Mouse)
• JE Burda, TM O'Shea, Y Ao, KB Suresh, S Wang, AM Bernstein, A Chandra, S Deverasett, R Kawaguchi, JH Kim, S McCallum, A Rogers, S Wahane, MV Sofroniew Divergent transcriptional regulation of astrocyte reactivity across disorders Nature, 2022-05-25;0(0):. 2022-05-25 [PMID: 35614216] (IHC, Mouse)
• Yaoming Wang, Kassandra Kisler, Angeliki Maria Nikolakopoulou, Jose A. Fernandez, John H. Griffin, Berislav V. Zlokovic 3K3A-Activated Protein C Protects the Blood-Brain Barrier and Neurons From Accelerated Ischemic Injury Caused by Pericyte Deficiency in Mice Frontiers in Neuroscience 2022-03-30 [PMID: 35431776]
• Q Zhao, W Dai, HY Chen, RE Jacobs, BV Zlokovic, BT Lund, A Montagne, A Bonnin Prenatal disruption of blood-brain barrier formation via cyclooxygenase activation leads to lifelong brain inflammation Proceedings of the National Academy of Sciences of the United States of America, 2022-04-04;119(15):e2113310119. 2022-04-04 [PMID: 35377817] (IHC-F, Mouse)
• YG Choe, JH Yoon, J Joo, B Kim, SP Hong, GY Koh, DS Lee, WY Oh, Y Jeong Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain Frontiers in Cellular Neuroscience, 2022-03-11;16(0):848764. 2022-03-11 [PMID: 35360491] (IHC, Mouse)
• T Higa, Y Okita, A Matsumoto, S Nakayama, T Oka, O Sugahara, D Koga, S Takeishi, H Nakatsumi, N Hosen, S Robine, MM Taketo, T Sato, KI Nakayama Spatiotemporal reprogramming of differentiated cells underlies regeneration and neoplasia in the intestinal epithelium Nature Communications, 2022-03-21;13(1):1500. 2022-03-21 [PMID: 35314700] (Flow Cytometry, IHC-F, Mouse)
• Axel Montagne, Angeliki M. Nikolakopoulou, Mikko T. Huuskonen, Abhay P. Sagare, Erica J. Lawson, Divna Lazic et al. APOE4 accelerates advanced-stage vascular and neurodegenerative disorder in old Alzheimer’s mice via cyclophilin A independently of amyloid-beta Nature Aging 2022-03-16 [PMID: 35291561]
• TA Sakaue, Y Fujishima, Y Fukushima, Y Tsugawa-Sh, S Fukuda, S Kita, H Nishizawa, B Ranscht, K Nishida, N Maeda, I Shimomura Adiponectin accumulation in the retinal vascular endothelium and its possible role in preventing early diabetic microvascular damage Scientific Reports, 2022-03-09;12(1):4159. 2022-03-09 [PMID: 35264685] (Western Blot, Mouse)
• TR Womack, CT Vollert, O Ohia-Nwoko, M Schmitt, S Montazari, TL Beckett, D Mayerich, MP Murphy, JL Eriksen Prostacyclin Promotes Degenerative Pathology in a Model of Alzheimer's Disease Frontiers in Cellular Neuroscience, 2022-02-07;16(0):769347. 2022-02-07 [PMID: 35197825] (IHC, Mouse)
• AI Hicks, S Kobrinsky, S Zhou, J Yang, M Prager-Kho Anatomical Organization of the Rat Subfornical Organ Frontiers in Cellular Neuroscience, 2021-09-06;15(0):691711. 2021-09-06 [PMID: 34552469] (IHC, Rat)
• K Bisht, KA Okojie, K Sharma, DH Lentferink, YY Sun, HR Chen, JO Uweru, S Amancherla, Z Calcuttawa, AB Campos-Sal, B Corliss, L Jabbour, J Benderoth, B Friestad, WA Mills, BE Isakson, MÈ Tremblay, CY Kuan, UB Eyo Capillary-associated microglia regulate vascular structure and function through PANX1-P2RY12 coupling in mice Nature Communications, 2021-09-06;12(1):5289. 2021-09-06 [PMID: 34489419] (IHC, Mouse)
• Johanna Schaffenrath, Sheng-Fu Huang, Tania Wyss, Mauro Delorenzi, Annika Keller Characterization of the blood–brain barrier in genetically diverse laboratory mouse strains Fluids and Barriers of the CNS 2021-07-28 [PMID: 34321020]
• MR Williamson, CJA Fuertes, AK Dunn, MR Drew, TA Jones Reactive astrocytes facilitate vascular repair and remodeling after stroke Cell Reports, 2021-04-27;35(4):109048. 2021-04-27 [PMID: 33910014] (IHC, Mouse)
• Y Wu, H Wu, J Zeng, B Pluimer, S Dong, X Xie, X Guo, T Ge, X Liang, S Feng, Y Yan, JF Chen, N Sta Maria, Q Ma, F Gomez-Pini, Z Zhao Mild traumatic brain injury induces microvascular injury and accelerates Alzheimer-like pathogenesis in mice Acta neuropathologica communications, 2021-04-23;9(1):74. 2021-04-23 [PMID: 33892818] (IHC, Mouse)
• Angeliki Maria Nikolakopoulou, Yaoming Wang, Qingyi Ma, Abhay P. Sagare, Axel Montagne, Mikko T. Huuskonen et al. Endothelial LRP1 protects against neurodegeneration by blocking cyclophilin A Journal of Experimental Medicine 2021-04-05 [PMID: 33533918]
• S Guérit, E Fidan, J Macas, CJ Czupalla, R Figueiredo, A Vijikumar, BH Yalcin, S Thom, P Winter, H Gerhardt, K Devraj, S Liebner Astrocyte-derived Wnt growth factors are required for endothelial blood-brain barrier maintenance Progress in neurobiology, 2020-12-28;0(0):101937. 2020-12-28 [PMID: 33383106] (ICC, IHC, Mouse)
• TM O?Shea, AL Wollenberg, JH Kim, Y Ao, TJ Deming, MV Sofroniew Foreign body responses in mouse central nervous system mimic natural wound responses and alter biomaterial functions Nature Communications, 2020-12-04;11(1):6203. 2020-12-04 [PMID: 33277474] (IHC, Mouse)
• Kassandra Kisler, Angeliki M. Nikolakopoulou, Melanie D. Sweeney, Divna Lazic, Zhen Zhao, Berislav V. Zlokovic Acute Ablation of Cortical Pericytes Leads to Rapid Neurovascular Uncoupling Frontiers in Cellular Neuroscience 2020-02-14 [PMID: 32116568] (Immunohistochemistry, Mouse)
• Tomoya Shibahara, Tetsuro Ago, Kuniyuki Nakamura, Masaki Tachibana, Yoji Yoshikawa, Motohiro Komori et al. Pericyte-Mediated Tissue Repair through PDGFR beta Promotes Peri-Infarct Astrogliosis, Oligodendrogenesis, and Functional Recovery after Acute Ischemic Stroke eNeuro 2020-02-05 [PMID: 32046974]
• L Pous, SS Deshpande, S Nath, S Mezey, SC Malik, S Schildge, C Bohrer, K Topp, D Pfeifer, F Fernández-, S Doostkam, DK Galanakis, V Taylor, K Akassoglou, C Schachtrup Fibrinogen induces neural stem cell differentiation into astrocytes in the subventricular zone via BMP signaling Nat Commun, 2020-01-31;11(1):630. 2020-01-31 [PMID: 32005867] (IHC, Human, Mouse)
• S Grubb, C Cai, BO Hald, L Khennouf, RP Murmu, AGK Jensen, J Fordsmann, S Zambach, M Lauritzen Precapillary sphincters maintain perfusion in the cerebral cortex Nat Commun, 2020-01-20;11(1):395. 2020-01-20 [PMID: 31959752] (IHC, Mouse)
• RM Rajani, J Ratelade, V Domenga-De, Y Hase, H Kalimo, RN Kalaria, A Joutel Blood brain barrier leakage is not a consistent feature of white matter lesions in CADASIL Acta Neuropathol Commun, 2019-11-21;7(1):187. 2019-11-21 [PMID: 31753008] (IHC, Mouse)
• Giuseppe Faraco, Karin Hochrainer, Steven G. Segarra, Samantha Schaeffer, Monica M. Santisteban, Ajay Menon et al. Dietary salt promotes cognitive impairment through tau phosphorylation Nature 2019-10-31 [PMID: 31645758]
• Kuniyuki Nakamura, Tomoko Ikeuchi, Kazuki Nara, Craig S. Rhodes, Peipei Zhang, Yuta Chiba et al. Perlecan regulates pericyte dynamics in the maintenance and repair of the blood–brain barrier Journal of Cell Biology 2019-10-07 [PMID: 31541017] (Immunohistochemistry, Transgenic Mouse)
• DY Chen, NH Sun, YP Lu, LJ Hong, TT Cui, CK Wang, XH Chen, SS Wang, LL Feng, WX Shi, K Fukunaga, Z Chen, YM Lu, F Han GPR124 facilitates pericyte polarization and migration by regulating the formation of filopodia during ischemic injury Theranostics, 2019-08-14;9(20):5937-5955. 2019-08-14 [PMID: 31534530] (IHC, Mouse)
• Qingbin Wu, Xiaochen Yuan, Jing Bai, Ruiqin Han, Zhigang Li, Honggang Zhang et al. MicroRNA-181a protects against pericyte apoptosis via directly targeting FOXO1: implication for ameliorated cognitive deficits in APP/PS1 mice Aging (Albany NY) 2019-08-29 [PMID: 31467256]
• A Ayyaz, S Kumar, B Sangiorgi, B Ghoshal, J Gosio, S Ouladan, M Fink, S Barutcu, D Trcka, J Shen, K Chan, JL Wrana, A Gregorieff Single-cell transcriptomes of the regenerating intestine reveal a revival stem cell Nature, 2019-04-24;0(0):. 2019-04-24 [PMID: 31019301] (IHC, Mouse)
• V Bellver-La, F Bretheau, B Mailhot, N Vallières, M Lessard, ME Janelle, N Vernoux, MÈ Tremblay, T Fuehrmann, MS Shoichet, S Lacroix Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury Nat Commun, 2019-01-31;10(1):518. 2019-01-31 [PMID: 30705270] (IHC, Mouse)
• S Hendrikx, S Coso, B Prat-Luri, L Wetterwald, A Sabine, CA Franco, S Nassiri, N Zangger, H Gerhardt, M Delorenzi, TV Petrova Endothelial Calcineurin Signaling Restrains Metastatic Outgrowth by Regulating Bmp2 Cell Rep, 2019-01-29;26(5):1227-1241.e6. 2019-01-29 [PMID: 30699351] (IHC, Mouse)
• Cuihong Jia, Matthew P. Keasey, Hannah M. Malone, Chiharu Lovins, Richard R. Sante, Vlad Razskazovskiy et al. Vitronectin from brain pericytes promotes adult forebrain neurogenesis by stimulating CNTF Experimental Neurology 2019-02-01 [PMID: 30408465]
• Q Ma, Z Zhao, AP Sagare, Y Wu, M Wang, NC Owens, PB Verghese, J Herz, DM Holtzman, BV Zlokovic Blood-brain barrier-associated pericytes internalize and clear aggregated amyloid-?42 by LRP1-dependent apolipoprotein E isoform-specific mechanism Mol Neurodegener, 2018-10-19;13(1):57. 2018-10-19 [PMID: 30340601] (IHC, Mouse)
• K Koizumi, Y Hattori, SJ Ahn, I Buendia, A Ciacciarel, K Uekawa, G Wang, A Hiller, L Zhao, HU Voss, SM Paul, C Schaffer, L Park, C Iadecola Apo?4 disrupts neurovascular regulation and undermines white matter integrity and cognitive function Nat Commun, 2018-09-19;9(1):3816. 2018-09-19 [PMID: 30232327] (ICC, Mouse)
• MA Anderson, TM O'Shea, JE Burda, Y Ao, SL Barlatey, AM Bernstein, JH Kim, ND James, A Rogers, B Kato, AL Wollenberg, R Kawaguchi, G Coppola, C Wang, TJ Deming, Z He, G Courtine, MV Sofroniew Required growth facilitators propel axon regeneration across complete spinal cord injury Nature, 2018-08-29;0(0):. 2018-08-29 [PMID: 30158698] (IHC, Mouse)
• W Zhu, W Chen, D Zou, L Wang, C Bao, L Zhan, D Saw, S Wang, E Winkler, Z Li, M Zhang, F Shen, S Shaligram, M Lawton, H Su Thalidomide Reduces Hemorrhage of Brain Arteriovenous Malformations in a Mouse Model Stroke, 2018-03-28;0(0):. 2018-03-28 [PMID: 29593101] (IHC-Fr, Mouse)
• R Nakazato, K Kawabe, D Yamada, S Ikeno, M Mieda, S Shimba, E Hinoi, Y Yoneda, T Takarada Disruption of Bmal1 impairs blood-brain barrier integrity via pericyte dysfunction J. Neurosci., 2017-09-14;0(0):. 2017-09-14 [PMID: 28912161] (Immunohistochemistry, Transgenic Mouse)
• AM Nikolakopo, Z Zhao, A Montagne, BV Zlokovic Regional early and progressive loss of brain pericytes but not vascular smooth muscle cells in adult mice with disrupted platelet-derived growth factor receptor-? signaling PLoS ONE, 2017-04-25;12(4):e0176225. 2017-04-25 [PMID: 28441414] (IHC-Fr, Mouse)
• Liqun He, Michael Vanlandewijck, Elisabeth Raschperger, Maarja Andaloussi Mäe, Bongnam Jung, Thibaud Lebouvier et al. Analysis of the brain mural cell transcriptome Scientific Reports 2016-10-11 [PMID: 27725773] (Immunohistochemistry, Mouse, Transgenic Mouse)
• Mary C. Wallingford, Hilary S. Gammill, Cecilia M. Giachelli Slc20a2 deficiency results in fetal growth restriction and placental calcification associated with thickened basement membranes and novel CD13 and laminin alpha 1 expressing cells Reproductive Biology 2016-03-01 [PMID: 26952749]
• Mary Catherine Wallingford, Jia Jun Chia, Elizabeth M. Leaf, Suhaib Borgeia, Nicholas W. Chavkin, Chenphop Sawangmake et al. SLC20A2 DEFICIENCY IN MICE LEADS TO ELEVATED PHOSPHATE LEVELS IN CEREBROSPINAL FLUID AND GLYMPHATIC PATHWAY-ASSOCIATED ARTERIOLAR CALCIFICATION, AND RECAPITULATES HUMAN IDIOPATHIC BASAL GANGLIA CALCIFICATION Brain Pathology 2017-01-01 [PMID: 26822507]
• Muramatsu R, Kuroda M, Matoba K, Lin H, Takahashi C, Koyama Y, Yamashita T Prostacyclin prevents pericyte loss and demyelination induced by lysophosphatidylcholine in the central nervous system. J Biol Chem, 2015-03-20;290(18):11515-25. 2015-03-20 [PMID: 25795781] (IHC, IHC-Fr, Mouse, Rat)
• van Stijn , Caroline, Kim , Jason, Barish , Grant D, Tietge , Uwe J F, Tangirala , Rajendra Adiponectin expression protects against angiotensin II-mediated inflammation and accelerated atherosclerosis. PLoS ONE, 2014-01-22;9(1):e86404. 2014-01-22 [PMID: 24466075] (Western Blot, Mouse)
• Sagare A, Bell R, Zhao Z, Ma Q, Winkler E, Ramanathan A, Zlokovic B Pericyte loss influences Alzheimer-like neurodegeneration in mice. Nat Commun, 2013-01-01;4(0):2932. 2013-01-01 [PMID: 24336108] (IHC-Fr, Mouse)

Review for Aminopeptidase N/CD13 Antibody (AF2335) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 07/25/2022

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse brain
• Species: Mouse
• Lot: VRR0322031

Comments

• Comments: Mouse brain tissue (12 micrometer thickness) was incubated overnight with AF2335, 1:500, in PBS 1x at 4C. The day after, the slide was washed 3 x PBS 1x and then incubated with secondary anti-goat antibody AlexaFluor 568-conjugated, 1:1000 for 2 hours at r.t. After counterstaining with DAPI, image was acquired with confocal microscopy. AF2335 signal identifies brain pericytes surrounding blood vessels.

Bioinformatics

• Gene Symbol: ANPEP
• Uniprot:
  • P97449()