![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin Brain Antibody (D) Composite.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0003.jpg "Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin Brain Antibody (D) Composite.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | S105-13 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | Synthetic peptide amino acids 203-496 of human Ankyrin-B |
| Localization | Cytoplasm , Cytoskeleton , Myofibril , Sarcomere , M line , Apical Cell Membrane , Cell Membrane |
| Specificity | Detects 200kDa. No cross reactivity against Ankyrin-G. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ANK2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | ANK2 |
![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin B Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0002.jpg "Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin B Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm")
![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0004.jpg "Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")