Anti-Mouse Ig (H+L) Comp-Bead 3 Population (7.5 um) Kit

Anti-Mouse Ig (H+L) Comp-Bead 3 Population (7.5 um) Kit [NBP3-00499] - Histogram of flow cytometry data showing fron and side scatter and the three populations in the FITC channel. Dot plots show uncompensated and compensation populations in the FITC Log X PE Log.

• Applications: Flow

Anti-Mouse Ig (H+L) Comp-Bead 3 Population (7.5 um) Kit Summary

• Description: The Anti-Mouse Ig (H+L) Compensation Beads are designed to capture fluorophore-conjugated antibodies, serving as accurate and consistent compensation controls in multicolor flow cytometry.
  • Bind to antibodies of mouse, rat, and hamster origin and are immunoglobulin light chain independent
  • Compatible with UV- to near IR-excited fluorophores
  • Exhibit low autofluorescence regardless of excitation wavelength (e.x. violet laser or red laser) or detection bandpass
  • Contains 3 populations (negative, low, high) for comprehensive quality control of fluorophores
  Concentration: 2.5 x 10^6 particles / mL
  
  Particle size: 7.0 -7.9 micron
  
  Particle material: polystyrene beads coated with goat anti-mouse IgG
• Kit Type: Kit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS, 0.2% BSA
• Preservative: 0.02% Sodium Azide

Alternate Names for Anti-Mouse Ig (H+L) Comp-Bead 3 Population (7.5 um) Kit

• Comp Beads
• Compensation Beads
• Compensation Capture Beads
• Flow Cytometry Compensation

Background

Compensation is the mathematical correction for the amount of overlap (spillover) of one fluorochrome's emission spectra into another fluorochrome's channel. To calculate this overlap, record single-color stained controls for each fluorochrome in a multicolor panel. This allows the cytometer to visualize the emission of each fluorochrome in each channel, or detector, and subtract the spillover, generating a matrix to apply to multi-color experimental samples. To ensure accurate calculations, single-color controls need to be as bright, or brighter, than the marker on the cell of interest. With increased use of multi-parametric flow cytometry and the large number of fluorochromes available, it is more important than ever to ensure accurate compensation for reliable interpretation of data and reproducible experimental results.

Compensation beads are a highly desirable alternative to traditional cell-based single-color compensation. Positive beads can come pre-loaded with anti-IgG for conjugated antibody capture of mouse, rat, or hamster origin. Uncoated (negative) beads ensure standardization of the autofluorescence in each channel. Beads are preferable to cell-based compensation for several reasons:

1) No precious sample is wasted, and more sample can be used for experimental acquisition.

2) Since 100% of positive beads are able to capture the antibody, the exact experimental fluorochrome can be used regardless of a marker's cellular expression. Dimly expressed markers may not be bright enough for compensation using cells.

3) Reduced autofluorescence of beads allows for more precise calculation of spectral overlap.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.