Mouse Apelin ELISA Kit (Colorimetric)

ELISA: Mouse Apelin ELISA Kit (Colorimetric) [NBP2-68235] - Samples were spiked with high concentrations of Mouse Apelin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Apelin ELISA Kit (Colorimetric) [NBP2-68235] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 125.00 - 8000 pg/mL
• Sensitivity: 75.00 pg/mL

Mouse Apelin ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse APLN in samples. No significant cross-reactivity or interference between Mouse APLN and analogues was observed.
• Standard Curve Range: 125.00 - 8000 pg/mL
• Sensitivity: 75.00 pg/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: CV% < 4.7%
• Intra-Assay: CV% < 4.76%
• Spike Recovery: 86-108%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: APLN

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Apelin ELISA Kit (Colorimetric)

• AGTRL1 ligand
• AGTRL1 Lligand
• APEL
• Apelin
• apelin, AGTRL1 ligand
• APJ endogenous ligand
• APLN
• XNPEP2

Background

Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei and is an endogenous ligand for APJ, a G protein-coupled orphan receptor which is an alternative coreceptor with CD4 for HIV-1. Apelin and APJ are ubiquitously expressed in peripheral tissues, with highest levels reported for heart and lungs, as well several regions within the central nervous system. The actions of apelin remain largely unknown: Apelin inhibits HIV-1 entry in cells coexpressing CD4 and APJ; the oral intake of Apelin in colostrum and breast milk could have a role in the modulation of the immune responses in neonates; more recent studies have also indicated a role for Apelin in the central control of body fluid homeostasis, by influencing AVP release and drinking behavior. In the cardiovascular system several actions of Apelin have been described, including endothelium-dependent vasodilatation, vasoconstriction through direct action on smooth muscle and positive inotropism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Apelin ELISA Kit (NBP2-68235)(1)

Publication using NBP2-68235

• Strutt B, Szlapinski S, Gnaneswaran T Et Al. Ontology of the apelinergic system in mouse pancreas during pregnancy and relationship with beta-cell mass Scientific reports 2021-07-29 [PMID: 34326390]

Bioinformatics

• Gene Symbol: APLN