APO-BRDU (TUNEL) Apoptosis Kit

APO-BRDU (TUNEL) Apoptosis Kit [NBP2-31161] - Figure showing the addition of Br-dUTP catalyzed by TdT to the 3'-OH sites of DNA strand breaks induced in the genome of apoptotic cells.

APO-BRDU (TUNEL) Apoptosis Kit [NBP2-31161] - Figure showing apoptotic cells undergoing apoptosis in S-phase.

• Applications: Flow, ICC/IF

APO-BRDU (TUNEL) Apoptosis Kit Summary

• Description: The APO-BRDU™ kit is a two color TUNEL assay for labeling DNA breaks (FITC) along with total cellulalr DNA (Propidium Iodide) to detect apoptotic cells. The kit contains all necessary reagents including positive and negative controls.
• Kit Type: Apoptosis Kit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: Please centrifuge the TdT Enzyme for 30 seconds, upon warming, to ensure all liquid is at the bottom of the tube.

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for APO-BRDU (TUNEL) Apoptosis Kit

• APO BRDU
• APO-BRDU (TUNEL) Assay
• APO-BRDU
• TUNEL Assay

Background

Apoptosis is the term that describes regulated cell death. It is believed to take place in the majority of animal cells. It is a distinct event that triggers characteristic morphological and biological changes in the cellular life cycle. It is common during embryogenesis, normal tissue and organ involution, cytotoxic immunological reactions and occurs naturally at the end of the life span of differentiated cells. It can also be induced in cells by the application of a number of different agents including physiological activators, heat shock, bacterial toxins, oncogenes, chemotherapeutic drugs, ultraviolet and gamma radiation. When apoptosis occurs, the nucleus and cytoplasm of the cell often fragments into membrane-bound apoptotic bodies that are then phagocytized by neighboring cells. An alternative mode of cell death, necrosis, occurs as a result of gross injury to cells resulting in cellular lysing and release of cytoplasmic components into the surrounding environment, Necrosis often induces an inflammatory response in the tissue. A landmark of cellular self-destruction by apoptosis is the activation of nucleases that degrade the higher order chromatin structure of the DNA into fragments of 50 to 300 kilobases and subsequently into smaller DNA pieces of about 200 base pairs in length. Numerous reviews of the events accompanying apoptosis are available and several well-researched model systems have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for APO-BRDU (TUNEL) Kit (NBP2-31161)(4)

Publications using NBP2-31161

• Choudhary P, Roy T, Chatterjee A et al. Melatonin rescues swim stress induced gastric ulceration by inhibiting matrix metalloproteinase-3 via down-regulation of inflammatory signaling cascade Life sciences 2022-02-23 [PMID: 35218765]
• Xu J, Su SM, Zhang X et al. ATP11B inhibits breast cancer metastasis in a mouse model by suppressing externalization of nonapoptotic phosphatidylserine Journal of Clinical Investigation 2022-03-01 [PMID: 35025764] (Human, Cell Apoptosis)
• Higashi Y, Matsumoto K, Saitoh H Et Al. Iodine containing porous organosilica nanoparticles trigger tumor spheroids destruction upon monochromatic X-ray irradiation: DNA breaks and K-edge energy X-ray Scientific reports 2021-07-14 [PMID: 34262055]
• Yuan B, Broadbent JA, Huan J et al. The Effects of Adipose Stem Cell-Conditioned Media on Fibrogenesis of Dermal Fibroblasts Stimulated by Transforming Growth Factor-beta 1 J Burn Care Res 2018-01-01 [PMID: 29931303] (CA, Human)

FAQs for APO-BRDU (TUNEL) Kit (NBP2-31161). (Showing 1 - 2 of 2 FAQs).

1. How many tests can you perform with this kit?
   • 60 assays
2. Can this detect bacteria? 
   • It is a two color assay for labeling DNA breaks and total cellular DNA to detect apooptotic cells by flow and microscopy. It will detect any cellular DNA and DNA with breaks. For more information please see the manual