Apolipoprotein B/ApoB Antibody (7B8) - BSA Free

Immunocytochemistry/Immunofluorescence: Apolipoprotein B/ApoB Antibody (7B8) [NBP2-23519] - Staining in human kidney tissue. Signal is strong in veins of glomerulus. Dilution: 1:100.

Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody (7B8) [NBP2-23519] - Staining of human tonsil tissue. Plasma of the veins were stained strongly. Dilution: 1:400

Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody (7B8) [NBP2-23519] - Staining of human kidney tissue. Dilution: 1:400

Product Discontinued

Apolipoprotein B/ApoB Antibody (7B8) - BSA Free Summary

• Immunogen: Human LDL
• Epitope: Epitope differs from that of Clone 03 and Clone 04
• Specificity: Clone 02 reacts with apo B from human serum/plasma, when used in sandwich ELISA in combination with a polyclonal antibody against apo B, and also with isolated LDL directly coated onto the microtiter plate. In Western blotting after SDS-PAGE Clone 02 binds to apo B in reduced as well as in non-reduced forms, using isolated LDL as antigen.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: APOB
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Apolipoprotein B/ApoB Antibody (7B8) - BSA Free

• Apo B-100
• APOB
• ApoB-100
• ApoB-48
• apolipoprotein B (including Ag(x) antigen)
• Apolipoprotein B
• apolipoprotein B-100
• apolipoprotein B48
• FLDB
• LDLCQ4
• mutant Apo B 100

Background

Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL). The molecular mass is 260 kDa. Apo B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APOB