APPBP1 Antibody (2E9-D12) - Azide and BSA Free

Western Blot: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody (2E9-D12) [H00008883-M02] - Analysis of NAE1 expression in transfected 293T cell line by NAE1 monoclonal antibody (M02), clone 2E9-D12.Lane 1: NAE1 transfected lysate (Predicted MW: 60.2 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody (2E9-D12) [H00008883-M02] - Detection limit for recombinant GST tagged NAE1 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 2E9-D12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

APPBP1 Antibody (2E9-D12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: APPBP1 (AAH00480, 1 a.a. ~ 534 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  
  MAQLGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NAE1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot
• Application Notes: It has been used for ELISA and WB.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for APPBP1 Antibody (2E9-D12) - Azide and BSA Free

• A-116A10.1
• amyloid beta precursor protein binding protein 1, 59kDa
• Amyloid beta precursor protein-binding protein 1, 59 kDa
• amyloid beta precursor protein-binding protein 1, 59kD
• APPBP1
• APP-BP1
• HPP1
• NAE1
• NEDD8 activating enzyme E1 subunit 1
• NEDD8-activating enzyme E1 regulatory subunit
• NEDD8-activating enzyme E1 subunit
• protooncogene protein 1
• Proto-oncogene protein 1
• ula-1

Background

The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAE1
• Uniprot:
  • AAH00480(Human)