| Description | The ARE/LUCPoter(TM) reporter cell line is designed to monitor the induction of ARE and can be used for screening of agonists, antagonists or signaling inhibitors of ARE induction (Nrf2 activity) as well as for studying the Keap1/Nrf2 signaling pathways.
Contents: 3-4 x 10^6 cells Biosafety Level: 1 |
| Immunogen | The ARE/LUCPorter(TM) reporter cell line is a stably transfected MCF7 cell line which expresses an optimized Renilla luciferase reporter gene (RenSP) under the transcriptional control of the antioxidant response element (ARE). ARE is known to regulate expression and induction of various detoxifying enzyme genes in response to antioxidants and xenobiotics, and is primarily regulated by the Keap1-Nrf2 pathway in which induction and nuclear translocation of Nrf2 mediated by antioxidants and xenobiotics results in the binding of Nrf2 to ARE leading to the expression of defensive genes. One of the antioxidants, curcumin, is known to upregulate Nrf2 leading to activation of the AREs. As also shown in Figure 1, the ARE/ LUCPorter(TM) reporter cell line can be activated by curcumin, in which curcumin activated the cell line in a dose response manner (Figure 1). |
| Selection Agent | Puromycin at 1 ug/ml |
| Growth Properties | Adherent Morphology : Epithelial |
| RCL Type | Luciferase - (LUCPorter™) |
| Host | MCF7 |
| Storage | Store in gas phase of liquid nitrogen. |
| Reconstitution Instructions | Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin (Note: The selection agents for this cell line is puromycin at 1 ug/ml). |
